15,242 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Full text linkThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
A strong temperature dependent hole direct tunneling current in p+ gate/pMOSFET with ultra-thin gate oxide
No full textFatigue resistance analysis of tibial baseplate in total knee prosthesis- an in vitro biomechanical study
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PRONUNCIATION CHALLENGES OF MANDARIN PHONEMES ZH, CH, SH, D, AND T AMONG SMPIT CHENG HOO MAKASSAR STUDENTS
Full text linkThe differences in the phonological systems between Indonesian and Mandarin often cause difficulties for learners, especially in the consonant sounds zh, ch, sh, d, and t which do not have direct equivalents in Indonesian. These pronunciation difficulties, especially in junior high school students, are not free from errors in pronouncing the consonants h, ch, sh, d, and t, so this study shows and describes the form of pronunciation difficulties of the consonants zh, ch, sh, d, and t directly in grade IX students of SMP IT Cheng Hoo Makassar, and identifies the factors causing these difficulties. The research method used in this study is descriptive qualitative with 15 students as subjects. Data were collected through pronunciation tests, syllable tests, observations, structured interviews, and audio recordings. The results showed that most students had difficulty pronouncing the retroflex consonants zh and ch, as well as the aspirated consonant t. Errors that emerged included difficulties in tongue articulation, failure to distinguish between aspirated and non-aspirated sounds, and interference from Indonesian, for example, zh is pronounced as j and t is pronounced as d. Physiological factors such as a stiff tongue position also affect pronunciation accuracy. In conclusion, ninth-grade students of SMP IT Cheng Hoo Makassar still face significant challenges in pronouncing certain consonants in Mandarin. These findings emphasize the need for more targeted phonetic teaching strategies, such as explicit articulation exercises and the use of audio-visual media, so that students can improve their accuracy and confidence in speaking Mandarin
Luminescence characteristics of sol-gel derived Y3Al5O12 : Eu3+ phosphors excited with vacuum ultraviolet
No full textIsolation and characterization of Vibrio-damsela infectious for cultured shrimp in Taiwan.
No full textGrowth of RA-CH-1pLMF03, RA-CH-1<i>ΔB739_1343</i>pLMF03, and RA-CH-1<i>ΔB739_1343</i>pLMF03::<i>B739_1343</i> on TSA and TSA supplemented with 50 μM Dip.
No full textThe R. anatipestifer strains (clockwise from top left) RA-CH-1pLMF03, RA-CH-1ΔB739_1343pLMF03, and RA-CH-1ΔB739_1343pLMF03::B739-1343 were grown on TSA plates containing cefoxitin (1 μg/mL) and 0 μM Dip (A) or 50 μM Dip (B). Growth was assessed by the appearance of bacterial colonies on plates. Pictures were taken after 48 h of growth at 37°C. All the experiments were repeated three times. Representative plates are presented.</p
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