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CUHK electronic theses & dissertations collection
No full textby Cheng Ting."August 2004."Thesis (Ph.D.)--Chinese University of Hong Kong, 2004.Includes bibliographical references (p. 103-107).Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web.Mode of access: World Wide Web.Abstracts in English and Chinese
Cheng Ting Au. (1993) : NG Ting Au. (1993) ; Cheung Yat-shing, Gan Yu'en : A Bibliography of Yue Dialect Studies I Cheung Yat-shing
No full textBauer Robert S. Cheng Ting Au. (1993) : NG Ting Au. (1993) ; Cheung Yat-shing, Gan Yu'en : A Bibliography of Yue Dialect Studies I Cheung Yat-shing. In: Cahiers de linguistique - Asie orientale, vol. 24 1, 1995. pp. 183-188
Cheng Ting Au. (1993) : NG Ting Au. (1993) ; Cheung Yat-shing, Gan Yu'en : A Bibliography of Yue Dialect Studies I Cheung Yat-shing
No full textBauer Robert S. Cheng Ting Au. (1993) : NG Ting Au. (1993) ; Cheung Yat-shing, Gan Yu'en : A Bibliography of Yue Dialect Studies I Cheung Yat-shing. In: Cahiers de linguistique - Asie orientale, vol. 24 1, 1995. pp. 183-188
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Characterization and Immunogenicity of Hepatitis C Virus Envelope Proteins
No full text全世界大約有百分之三的人口感染C型肝炎病毒(HCV)。服用Ribavirin並同時注射聚乙烯二醇化甲型干擾素(pegylated interferon-α)是目前的治療方法，但是此療法對不同基因型C型肝炎病毒的感染有不同效果。然而現今仍然沒有可對抗或預防C型肝炎病毒的疫苗，因此如何製備出有效的疫苗是一大課題。
C型肝炎病毒的基因組可轉譯出一條多蛋白(polyprotein)，並且能被宿主以及病毒本身的蛋白酶切割成多段結構蛋白以及非結構蛋白。其中，包膜蛋白(envelope protein)屬於結構蛋白，表現在病毒的表面上，包含了E1及E2兩個蛋白，並且在結合受體(receptor)與病毒進入細胞上扮演了重要的角色。E1和E2蛋白是高度醣化(glycosylated)的醣蛋白，蛋白質的醣化對於蛋白質折疊以及受體的結合來說都十分重要，不過醣也可以遮蔽蛋白質上重要的、具有功能性的位置，讓宿主無法對病毒產生有效的抗體來對抗病毒的感染。
我的研究是探討醣在C型肝炎病毒包膜蛋白E1以及E2醣蛋白的角色。我先從人類293T細胞中製造且純化出全長(full-length)的E1E2膜蛋白，以及去除部分C端(C-terminal truncated)的E2蛋白。接著，再利用醣內切酶 H (Endoglycosidase H, Endo H)酵素去除這兩種蛋白上除了第一個乙醯葡萄糖胺(N-acetylglucosamine)之外大部分的醣，這樣處理後的蛋白即為單醣形式(mono-glycosylated form)，而沒有用醣內切酶 H處理過的包膜蛋白，稱之為全醣形式(fully-glycosylated form)。接著我比較了這兩種不同醣形式的全長E1E2以及C端部分切除的E2蛋白上的醣化程度及種類，確定醣是否切除的完整；並用圓二色光譜(circular dichroism)比較其二級結構，同時評估其對受體CD81的結合能力，探討切除了醣的蛋白是否仍然具有一樣的結構及活性。最後，並試圖探討C型肝炎病毒包膜醣蛋白引發老鼠免疫反應的能力。
實驗結果發現，單醣形式上有偵測到的醣化位置都只剩下一顆乙醯葡萄糖胺，而二級結構與全醣形式相似，與受體CD81結合能力則是明顯的比全醣形式來的高。另外，在引發免疫反應的部分，只有全醣形式可以引發出較高的抗體效價，而單醣形式的效果比較差。It was estimated that about 3% of the worldwide population were infected with hepatitis C virus (HCV). The current therapy is ribavirin combined with pegylated interferon-α, and the effect was differential for infections with various HCV genotypes. Until now there is still no vaccine available.
The genome of HCV encodes a polyprotein which could be cleaved into structural and nonstructural proteins by host and viral proteases. The envelope proteins are structural proteins which are expressed on the viral surface and are comprised of heterodimers of E1 and E2. They play an important role in receptor binding and virus entry. HCV E1 and E2 proteins are highly glycosylated. Glycans may be crucial for protein folding or receptor binding or both. Glycans also can mask the functionally important region of protein so that proteins may not produce effective antibodies to defeat the viruses.
Our ultimate goal is to investigate the roles of glycans on HCV E1/E2 glycoproteins, especially on the immunogenicity. Recombinant HCV envelope proteins were produced from human 293T cells. The purified proteins are either used directly (fully-glycosylated) or digested with Endoglycosidase H to remove all the glycans except the first GlcNAc (mono-glycosylated). The glycosylation patterns of fully- and mono-glycosylated HCV envelope proteins were further examined by glycan profiling. Their structural and functional properties were also evaluated with circular dichroism spectroscopy and with binding activity to CD81, a receptor of HCV. In addition, the immunogenicity of HCV envelope proteins in various forms was investigated.
The results showed that the N-glycans of mono-glycosylated proteins were mostly one HexNAc, and the secondary structures of fully- and mono-glycosylated proteins were similar. Furthermore, the CD81 binding activity of mono-glycosylated proteins was higher than fully-glycosylated proteins. However, the immunogenicity of fully-glycosylated proteins was higher than mono-glycosylated HCV envelope proteins
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Internal reference genes with the potential for normalizing quantitative PCR results for oral fluid specimens
No full textIn basic research, testing of oral fluid specimens by real-time quantitative polymerase chain reaction (qPCR) has been used to evaluate changes in gene expression levels following experimental treatments. In diagnostic medicine, qPCR has been used to detect DNA/RNA transcripts indicative of bacterial or viral infections. Normalization of qPCR using endogenous and exogenous reference genes is a well-established strategy for ensuring result comparability by controlling sample-to-sample variation introduced during sampling, storage, and qPCR testing. In this review, the majority of recent publications in human (n = 136) and veterinary (n = 179) medicine did not describe the use of internal reference genes in qPCRs for oral fluid specimens (52.9% animal studies; 57.0% human studies). However, the use of endogenous reference genes has not been fully explored or validated for oral fluid specimens. The lack of valid internal reference genes inherent to the oral fluid matrix will continue to hamper the reliability, reproducibility, and generalizability of oral fluid qPCR assays until this issue is addressed.This article is published as Cheng, Ting-Yu, Jeffrey J. Zimmerman, and Luis G. Giménez-Lirola. "Internal reference genes with the potential for normalizing quantitative PCR results for oral fluid specimens." Animal Health Research Reviews (2022): 1-10.
DOI: 10.1017/S1466252322000044.
Copyright 2022 The Author(s).
Attribution 4.0 International (CC BY 4.0).
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