741 research outputs found
sj-docx-2-tam-10.1177_17588359231225036 – Supplemental material for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis
No full textSupplemental material, sj-docx-2-tam-10.1177_17588359231225036 for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis by Jiayan Chen, Wanjun Lu, Mo Chen, Zijing Cai, Ping Zhan, Xin Liu, Suhua Zhu, Mingxiang Ye, Tangfeng Lv, Jiawen Lv, Yong Song and Dong Wang in Therapeutic Advances in Medical Oncology</p
sj-docx-3-tam-10.1177_17588359231225036 – Supplemental material for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis
No full textSupplemental material, sj-docx-3-tam-10.1177_17588359231225036 for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis by Jiayan Chen, Wanjun Lu, Mo Chen, Zijing Cai, Ping Zhan, Xin Liu, Suhua Zhu, Mingxiang Ye, Tangfeng Lv, Jiawen Lv, Yong Song and Dong Wang in Therapeutic Advances in Medical Oncology</p
sj-docx-1-tam-10.1177_17588359231225036 – Supplemental material for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis
No full textSupplemental material, sj-docx-1-tam-10.1177_17588359231225036 for Efficacy of immunotherapy in patients with oncogene-driven non-small-cell lung cancer: a systematic review and meta-analysis by Jiayan Chen, Wanjun Lu, Mo Chen, Zijing Cai, Ping Zhan, Xin Liu, Suhua Zhu, Mingxiang Ye, Tangfeng Lv, Jiawen Lv, Yong Song and Dong Wang in Therapeutic Advances in Medical Oncology</p
Supplemental Material - The Protective Effects of Apigenin Against Radiation‐Induced Intestinal Injury
No full textSupplemental Material for The Protective Effects of Apigenin Against Radiation‐Induced Intestinal Injury by Danjie Liu, Renjun Peng, Zhongmin Chen, Huijie Yu, Sinian Wang, Suhe Dong, Wei Li, Wen Shao, Jing Dai, Fengsheng Li, Qisheng Jiang and Wanjun Sun in Dose-Response</p
The role of CD4+ and CD8+ T cells in the Evolution of SCW Induced Arthritis and in Oral Tolerance
No full textvii, 31 p.Injection of Group A streptococcal cell wall (SCW) peptidoglycan-polysaccharide
complexes in susceptible rats induces acute inflammation in the joints followed by
chronic arthritis, producing an attractive animal model for rheumatoid arthritis.
Infiltration of neutrophils into the synovium characterizes the acute phase while
accumulation of T cells and monocytes mediates the chronic phase. In this study, we first
investigated the role of CD4+ and CD8+ T cells in the evolution of SCW-induced arthritis
by in vivo immuno-deletion of these cells. We found that neither CD4+ nor CD8+ T cells
affected acute inflammation. In contrast, both CD4 + and CD8+ T cells, at least in part,
regulated the chronic response. Unexpectedly, CD8+ T cells played a more important
role in the chronic phase because their deletion caused a dramatic increase in IL-4, an
anti-inflammatory cytokine. Importantly, oral administration of SCW is known to
suppress SCW induced arthritis in a dose dependent manner, however, the cells involved
in oral tolerance are ill defined. As our second objective, we assessed the same in vivo
immuno-deletion of CD4+ and CD8+ T cells. In orally tolerized animals, both CD4+ and
CD8+ immuno-deletion resulted in an increase in the acute response, but only a partially
resolved chronic inflammation. Immunohistochemical staining revealed that TGF-~, an
anti-inflammatory cytokine, was markedly increased in the orally tolerized gut tissue, but
not in the control animals. CD4+ and CD8+ immuno-deletion reduced the amount of
TGF- in the gut, compared to the tolerized animals. Therefore, our results demonstrated
that both CD4+ and CD8+ T cells play an inhibitory role in acute inflammation in oral
tolerance. In chronic inflammation, however, other factors may be important in helping
CD4+ or CD8+ T cells maintain tolerance.With honors.National Institute of Dental Research. National Institutes of Health. Bethesda, Maryland
Mesenchymal stem cell-organized bone marrow elements: an alternative hematopoietic progenitor resource
No full textBone marrow-derived mesenchymal stem cells (BMMSCs) are multipotent postnatal stem cells that have been used for the treatment of bone defects and graft-versus-host diseases in clinics. In this study, we found that subcutaneously transplanted human BMMSCs are capable of organizing hematopoietic progenitors of recipient origin. These hematopoietic cells expressed multiple lineages of hematopoietic cell associated markers and were able to rescue lethally irradiated mice, with successful engraftment in the recipient, suggesting a potential bone marrow (BM) resource for stem cell therapies. Furthermore, we found that platelet-derived growth factor (PDGF) promotes the formation of BMMSC-generated BM niches through upregulation of β-catenin, implying that the PDGF pathway contributes to the formation of ectopic BM. These results indicate that the BMMSC-organized BM niche system represents a unique hematopoietic progenitor resource possessing potential clinical value.Yasuo Miura, Zhigang Gao, Masako Miura, Byoung-Moo Seo, Wataru Sonoyama, Wanjun Chen, Stan Gronthos, Li Zhang, Songtao Sh
Hydroxylated graphene-based flexible carbon film with ultrahigh electrical and thermal conductivity
No full textGraphene-based films are widely used in the electronics industry. Here, surface hydroxylated graphene sheets (HGS) have been synthesized from natural graphite (NG) by a rapid and efficient molten hydroxide-assisted exfoliation technique. This method enables preparation of aqueous dispersible graphene sheets with a high dispersed concentration (similar to 10.0 mg ml(-1)) and an extraordinary production yield (similar to 100%). The HGS dispersion was processed into graphene flexible film (HGCF) through fast filtration, annealing treatment and mechanical compression. The HGS endows graphene flexible film with a high electrical conductivity of 11.5. x. 10(4)Sm(-1) and a superior thermal conductivity of 1842W m(-1) K-1. Simultaneously, the superflexible HGCF could endure 3000 repeated cycles of bending or folding. As a result, this graphene flexible film is expected to be integrated into electronic packaging and high-power electronics applications
Does the Stigma of Hooking Up Predict Sexual Assault at College?
No full textThis study examines the relationship between the stigma of hooking up and reported sexual assault. Guided by Goffman’s (1963) social stigma theory and Gagnon and Simon’s (1973) sexual script theory, I propose that 1) the more strongly the respondent agrees he or she would disrespect women who hook up frequently, the fewer times he or she reports nonconsensual sex; 2) the more strongly the respondent agrees he or she would disrespect for men who hook up frequently, the fewer times he or she reports nonconsensual sex; and 3) the more strongly the respondent agrees he or she would be less interested in someone who hooks up frequently as a boyfriend/girlfriend, the fewer times he or she reports nonconsensual sex. Using the Online College Social Life Survey data collected between 2005 and 2011, I analyze the attitudes about and reports of sexual behaviors in a non-probability sample of 16,914 students at 21 U.S. colleges and universities. Controlling for sex, age, current religion preference, and Greek affiliation, disrespect towards women who hook up frequently is positively and significantly related to fewer reports of nonconsensual sex. However, the results do not support the second and third hypotheses as there is no statistically significant relationship between disrespect towards men who hook up frequently as well as the lack of interest in people who hook up frequently and the incidents of reported nonconsensual sex. The findings suggest that the efforts to reduce the stigma of hooking up should be taken into consideration in rape prevention programming
Mesenchymal stem cell-mediated ectopic hematopoiesis alleviates aging-related phenotype in immunocompromised mice
No full textSubcutaneous transplants of bone marrow mesenchymal stem cells (BMMSCs) are capable of generating ectopic bone and organizing functional hematopoietic marrow elements in animal models. Here we report that immunocompromised mice received subcutaneous BMMSC transplants using hydroxyapatite tricalcium phosphate as a carrier suppressed age-related degeneration in multiple organs and benefited an increase in life span extension compared with control littermates. The newly organized ectopic bone/marrow system restores active hematopoiesis via the erythropoietin receptor/signal transducer and activator of transcription 5 (Stat5) pathway. Furthermore, the BMMSC recipient mice showed elevated level of Klotho and suppression of insulin-like growth factor I signaling, which may be the mechanism contributing to the alleviation of aging-like phenotypes and prolongation of life in the treated mice. This work reveals that erythropoietin receptor/Stat5 pathway contributes to BMMSC-organized ectopic hematopoiesis, which may offer a treatment paradigm of reversing age-related degeneration of multiple organs in adult immunocompromised mice.Takayoshi Yamaza, Yasuo Miura, Kentaro Akiyama, Yanming Bi, Wataru Sonoyama, Stan Gronthos, WanJun Chen, Anh Le and Songtao Sh
Adaptive immune responses in intestinal homeostasis and experimental inflammatory bowel disease
No full textThe intestine, a dwelling place for trillions of commensal bacteria, is constantly exposed to numerous ligands and antigens from foods and microbiota. Intestinal homeostasis involves a balance between anti-inflammatory and pro-inflammatory signals. Intestinal epithelial barrier, commensal bacteria, and immune cells, along with the interactions between the three players maintain a toleranic environment to food and microbiota. Conversely, when a dysregulated interaction occurs in genetically susceptible individuals, the homeostasis breaks down and chronic intestinal inflammation develops. CD4+ T cells are important mediators for both intestinal homeostasis and colitis development. The intestine is a preferential site for induction of Treg, Th1 and Th17 cells. The differentiation, plasticity and functions of CD4+ T cells in intestines are intensely studied, but still incompletely defined. The differentiation of peripherally induced Tregs and Th17 cell population is reciprocally regulated in the intestine. In addition to the regulation by master transcriptional factors and STATS signaling, the ERK pathway is also involved in regulating or fine-tuning T cell lineage commitment. ERK differentially regulates Tregs and Th17 cell development by positively regulating Th17 and negatively regulating Treg cell differentiation. My studies indicated that the inhibition of ERK decreased IL-6 induction of RORγt while it enhanced TGF-β induction of Foxp3. Moreover, ERK inhibitor-treated T cells under Th17 conditions possessed a suppressive function in vitro because they produced more IL-10 and TGF-β. Furthermore, ERK inhibitor-treated T cells under Th17 polarization conditions had a decreased potency to induce colitis in vivo. Although accumulating evidence demonstrates that differentiated CD4+ T cells preserve plasticity to alter phenotypes under various conditions, it is still unclear how stable Th1 cells are and whether Th1 cells can convert into Th17 cells. I demonstrated that Th1 cells converted into Th17 cells under inflammatory conditions in the mouse intestines. TGF-β, IL-6 and IL-2, but not hypoxia factors, differentially regulated Th1 to Th17 conversion. TGF-β induction of Runx1 and RORγt, was crucial for the conversion. Taken together, my studies revealed the interference with the ERK pathway could represent a therapeutic treatment for inflammatory bowel diseases and demonstrated that Th1 cells convert into Th17 cells under inflammatory conditions in intestines, which was mediated by TGF-β induction of Runx1
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