1,721,018 research outputs found
High Sensitivity IGZO Thin Film Transistor Biosensors for the Detection of Epstein-Barr Virus Protein
No full text在這篇論文中，我們以氧化銦鎵鋅薄膜電晶體並附有延伸金感測板構成生醫感測器檢測蛋白質。電晶體扮演著感應與訊號輸出的角色，而金感測板具有傳導蛋白質電荷到電晶體通道層以改變通道電流的功能。我們在此研究中測試了兩種蛋白質，一種則是小鼠血清裡的免疫球蛋白G，一種是人類皰疹病毒第四型(EBV)的抗體。在免疫球蛋白G的檢測中，我們利用氧化鋅奈米柱對感應區表面作改質，並藉由其高面積對體積比例而感善了感測器的靈敏度。在人類泡疹病毒第四型的檢測中，我們利用自組裝分子層及蛋白質交聯劑來作改質，為的是改善蛋白質的固定化。進一步地，我們還運用了上閘極電壓變化的分析來增加實驗的可靠性與可重複性。最後，我們將生醫感測器與目前現有的蛋白質檢測方法做比較，省時、高敏感度是我們感測器的顯著優勢。我們可以在10pg/ml離體的小鼠血清溶液中量測到濃度為8.98fM的小鼠免疫球蛋白G。並且專一性地量測到濃度為10pg/ml的EBV抗體溶液。A biosensor structure consisting of an Indium-Gallium-Zinc-Oxide (IGZO) thin film transistor (TFT) and a gold extended sensing pad was demonstrated. The IGZO-TFT was the device for signal extraction, and the sensing pad could conduct protein charges resulting channel current changed. There were two kinds of target protein in the thesis, mouse IgG in the mouse serum and Epstein-Barr virus antibodies. In the detection for mouse IgG, the biosensor was functionalized by applying ZnO nanorods on the pad to modify the gold surface. ZnO nanorods improved the sensitivity of the biosensors by high surface-to-volume ratio. In the detection for EBV antibodies, the device was functionalized by self-assembled monolayer and crosslinker to solve the problem of protein immobilization. Furthermore, the top gate voltage analysis was employed to improve reliability and repeatability of the experiment. Finally, the TFT-biosensor was compared with the conventional methods for protein detection. Time-saving and high sensitivity were the significant advantages of the TFT biosensor. The bio-TFT is able to selectively detect 10pg/ml mouse IgG in the serum solution and 10pg/ml EBV antibodies in the buffer solution.謝誌 I
摘要 II
Abstract III
Contents IV
List of Figure VI
List of Table IX
Chapter 1 Introduction 1
1.1 Background of Epstein-Barr virus detection 1
1.2 Overview of FET biosensors 4
1.3 Thesis structure 7
Chapter 2 Fabrication of the TFT-biosensors 8
2.1 Introduction 8
2.2 Structure and fabrication 9
2.3 Functionalization by ZnO nanorods 13
2.4 Functionalization by SAM and crosslinker 21
Chapter 3 Protein detection of IgG in mouse serum 23
3.1 Introduction 23
3.2 Quantification and analysis of the ZnO nanorods 24
3.3 Detection IgG in mouse serum by TFT-biosensors 27
Chapter 4 Protein detection of Epstein-Barr Virus 36
4.1 Introduction 36
4.2 Detection EBV antibodies in buffer by TFT-biosensors 37
4.4 Comparison of conventional methods 52
4.5 Summary 62
Chapter 5 Conclusion 63
Reference 6
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Safe Lives: Dealing with Earthquakes by open space in Taipei, Taiwan\u2028
No full textTaiwan is located in the Circum-Pacific Seismic Zone; therefore, this island nation is destined to encounter recurrent cataclysmic earthquakes as what has been witnessed in the previous centuries. Taipei City, which is recognized as the most populous and densest city of Taiwan, has a series of push factors which lead herself standing with massive quakes. Besides, with old buildings, disordered urban pattern and lack of open space not only makes the city expose in an extremely dangerous situation but an uncomfortable living environment. Based on the descriptions above, the research objective of this thesis is focused on the following main question: “What are the landscape interventions that can create a safe and livable city?” As a result, in this thesis, it is mainly developed into two layers, emergency and living quality layers, and then combine them as a complete landscape design in Taipei for a safer and better living quality environment.Flowscapes StudioArchitecture, Urbanism and Building Sciences | Landscape Architectur
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
The design of large separating angle ultracompact wavelength division demultiplexer based on photonic crystal ring resonators
No full text- …
