42 research outputs found
ASO Author Reflections: Minimally Invasive Approach and Oncologic Benefit in Intrahepatic Cholangiocarcinoma (iCCA) With Risk of Very Early Recurrence: Is it Time to Consider Technique as a Key Element of Onco-Surgical Strategy?
No full textThe Microbiome: A New Target for Research and Treatment of Schizophrenia and its Resistant Presentations? A Systematic Literature Search and Review
Get PDFBackground: The gastrointestinal system hosts roughly 1,800 distinct phyla and about 40,000 bacterial classes, which are known as microbiota, and which are able to influence the brain. For instance, microbiota can also influence the immune response through the activation of the immune system or through the release of mediators that are able to cross the brain blood barrier or that can interact with other substances that have free access to the brain, such as tryptophan and kynurenic acid, which is a metabolite of tryptophan and which has been involved in the pathogenesis of schizophrenia. Objectives: This paper reviews the possible relationships between microbiome, schizophrenia and treatment resistance. Given the possibility of a role of immune activation and alterations, we also describe the relationship between schizophrenia and immune inflammatory response. Finally, we report on the studies about the use of probiotic and prebiotics in schizophrenia. Methods: Cochrane library and PubMed were searched from the year 2000 to 2018 for publications about microbiome, immune-mediated pathology, schizophrenia and neurodevelopmental disorders. The following search string was used: (microbiome or immune mediated) AND (schizophrenia OR neurodevelopmental disorder). Associated publications were hand-searched from the list of references of the identified papers. A narrative review was also conducted about the use of probiotics and prebiotics in schizophrenia. Results: There exists a close relationship between the central nervous system and the gastrointestinal tract, which makes it likely that there is a relationship between schizophrenia, including its resistant forms, and microbiota. This paper provides a summary of the most important studies that we identified on the topic. Conclusions: Schizophrenia in particular, remain a challenge for researchers and practitioners and the possibility of a role of the microbiome and of immune-mediated pathology should be better explored, not only in animal models but also in clinical trials of agents that are able to alter gut microbiota and possibly influence the mechanisms of gastrointestinal inflammation. Microbiome targeted treatments have not been well-studied yet in patients with mental illness in general, and with schizophrenia in particular. Nonetheless, the field is well worth of being appropriately investigated. Copyright © 2018 Cuomo, Maina, Rosso, Beccarini Crescenzi, Bolognesi, Di Muro, Giordano, Goracci, Neal, Nitti, Pieraccini and Fagiolini. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
The treatment of the theme of identity in the works of Muthoni Likimani
No full textA thematic analysis of Muthoni Likimani's literary works reveals the author's
commitment to addressing identity as the focal point. This theme transcends all
her literary works. In our study, we examine how the author's pre-occupation
with identity becomes a lens through which the author perceives issues that are
prevalent in the society.
The study takes into consideration the fact that the author has not been subjected
to a comprehensive study. However, we acknowledge the author's inclusion in
feminist studies that identify her as a serious author whose works warrant literary
appreciation.
Our study in its examination of the treatment of the theme of identity is guided by
the tenets of the postcolonial and feminist literary theories. These theories are
selected for their particularised emphasis on the experiences of the colonised
society, and the woman respectively. The study essentially explores the settings,
perspective, voice and characterisation used as deliberate authorial manoeuvres
that expose her treatment of the theme of identity.
We focussed on patriarchal and colonial oppression as predominant experiences
that have permeated Likimani's creative writing. The sense of victimhood and
oppression necessitate an assertion of identity. The study therefore has examined
these elements and evaluated their impact on the author's definition of self-hood
and belonging. Apparently, Likimani in her writings concentrates on subverting
the structures of oppression that would infringe on one's sense of identity.University of Nairob
Never be silent : publishing & imperialism in Kenya, 1884-1963
Get PDFSocial communications are central to any social struggle. There is a sizable body of literature from other countries on the use of oral medium, newspapers, books and other forms of communications being used as tools for organising against a powerful enemy, as a training ground for cadres and for clarifying and developing revolutionary theory, ideology, organisation and practice. All this ensures a greater unity among those resisting oppression and exploitation. Thus revolutionary and liberation forces of Bolsheviks in the Soviet Union, the Communist Party of China, and in Vietnam had developed theories and practices of revolutionary publishing as part of their revolutionary work. This has also been the case during anti-colonial and anti-imperialist struggles in Africa, but very little of this has been systematically documented as an aspect of revolutionary communications policy and practice. While the
colonial communications systems have been reasonably well documented, the resistance communication systems remain largely undocumented and ignored. This book is an initial attempt to document this dynamic communications process in Kenya with its external struggles against colonialism and its complex internal struggles with overlaying divisions of race and class, Kenyan and foreign peoples. The main theme emerging from this experience is that people struggling to change their society always find ways of establishing their own system of communicating with the people they lead
and by whom they are led. Their mission of revolution, of change, of peace, of social and economic justice requires that they should never be silent. This was well understood and practised by the liberation forces in Kenya. They
were never silent
Draft genome of the filarial nematode parasite Brugia malayi
Get PDFParasitic nematodes that cause elephantiasis and river blindness threaten hundreds of millions of people in the developing world. We have sequenced the approximately 90 megabase (Mb) genome of the human filarial parasite Brugia malayi and predict approximately 11,500 protein coding genes in 71 Mb of robustly assembled sequence. Comparative analysis with the free-living, model nematode Caenorhabditis elegans revealed that, despite these genes having maintained little conservation of local synteny during approximately 350 million years of evolution, they largely remain in linkage on chromosomal units. More than 100 conserved operons were identified. Analysis of the predicted proteome provides evidence for adaptations of B. malayi to niches in its human and vector hosts and insights into the molecular basis of a mutualistic relationship with its Wolbachia endosymbiont. These findings offer a foundation for rational drug design
Impact of ownership of water resources and associated facilities to its access and management in the asal Kitui County, Kenya
No full textIn Kenya, where 80% of the land is arid and semi-arid, access to water is an everyday challenge for majority of the people. Methods used to improve access to water in Kenya results to different, and sometimes unexpected outcomes. The water resources assessed have come about through interventions by various agents, majority donor funded. These agents use various models resulting to different outcomes. We assessed technologies used to improve access to water in the Asals of Kitui County, Kenya. The technologies were classified into four; individual, private, community and government owned. Those assessed were tanks, boreholes and hand dug wells, sand dams and pipelines for individual, private, community and government owned respectively. Private and individual resources outperformed others in terms of management with donor aided community owned being the least sustainable. Government owned water facilities reached relatively more people and although unreliable were the most trusted source to those covered by the infrastructure. Individual owned water resources offered water in small quantities, the main benefit being owners were able to manage with ease through rationing to stretch availability for a long time. Donor funded community owned resources suffered vague ownership models making their management and maintenance impossible. Privately owned resources offered the best solution as water was sold and the resulting money become income for the owners as well as providing resources for maintenance. The resulting income offered incentives for further investment which further improved access. The tragedy with privately owned water resources was that the poor paid too much for water while the rich continued to accumulate massive wealth. To ensure adequate access to water especially in remote places, privately owned and operated systems should be encouraged and supported but with government sponsored regulations to ensure the poor are not exploited.Water Resource
Prevalence and risk factors of M tuberculosis infection in young people across 14 communities in Zambia and South Africa.
Get PDFBACKGROUND: From 2018-2021 the TB Reduction through Expanded Antiretroviral Treatment and TB Screening (TREATS) project took place in 21 Zambian and South African communities. The TREATS Incidence of TB Infection Cohort Study was conducted in adolescents and young people (AYP), aged 15-24 years in 14 communities. We describe the baseline prevalence and risk factors of Mycobacterium tuberculosis (M. tuberculosis) infection among this cohort and explore the quantitative QFT-Plus interferon gamma (IFN-γ) responses.
METHODS AND FINDINGS: A random sample of approximately 300 AYP per community were recruited and information on TB/HIV risk factors, TB symptoms and social mixing patterns collected. QuantiFERON TB Gold Plus assay (QFT-Plus) was used to detect M. tuberculosis infection, following manufacturer's instructions. Logistic regression was used to determine factors associated with infection. 5577 eligible AYP were invited to participate across both countries, with 4648 enrolled. QFT-Plus results were available for 4529: 2552(Zambia) and 1977(South Africa). Overall, 47.6% (2156/4529) AYP had positive QFT-Plus results, the prevalence of infection in South Africa being twice that in Zambia (64.7% (1280/1977) vs 34.3% (867/2552) p0.6 IU/ml) of the assay at baseline showed no evidence of association with recent TB exposure.
CONCLUSION: The high prevalence of infection in AYP warrants urgent action to address TB control, especially in South Africa. Further research is required to delineate antigen tube responses of the QFT-Plus assay more precisely to fully realise the benefit of the additional TB2 tube in high TB/HIV burden settings
Preclinical pharmacokinetics, biodistribution, radiation dosimetry and toxicity studies required for regulatory approval of a phase I clinical trial with 111In-CP04 in medullary thyroid carcinoma patients
Get PDFIntroduction: From a series of radiolabelled cholecystokinin (CCK) and gastrin analogues, 111In-CP04 (111In-DOTA-(DGlu)6-Ala-Tyr-Gly-Trp-Met-Asp-Phe-NH2) was selected for further translation as a diagnostic radiopharmaceutical towards a first-in-man study in patients with medullary thyroid carcinoma (MTC). A freeze-dried kit formulation for multicentre application has been developed. We herein report on biosafety, in vivo stability, biodistribution and dosimetry aspects of 111In-CP04 in animal models, essential for the regulatory approval of the clinical trial. Materials and methods: Acute and extended single dose toxicity of CP04 was tested in rodents, while the in vivo stability of 111In-CP04 was assessed by HPLC analysis of mouse blood samples. The biodistribution of 111In-CP04 prepared from a freeze-dried kit was studied in SCID mice bearing double A431-CCK2R(±) xenografts at 1, 4 and 24h pi. Further 4-h animal groups were either additionally treated with the plasma expander gelofusine or injected with 111In-CP04 prepared by wet-labelling. Pharmacokinetics in healthy mice included the 30min, 1, 4, 24, 48 and 72h time points pi. Dosimetric calculations were based on extrapolation of mice data to humans adopting two scaling models. Results: CP04 was well-tolerated by both mice and rats, with an LD50 >178.5μg/kg body weight for mice and a NOAEL (no-observed-adverse-effect-level) of 89μg/kg body weight for rats. After labelling, 111In-CP04 remained >70% intact in peripheral mouse blood at 5min pi. The uptake of 111In-CP04 prepared from the freeze-dried kit and by wet-labelling were comparable in the A431-CCK2R(+)-xenografts (9.24±1.35%ID/g and 8.49±0.39%ID/g, respectively; P >0.05). Gelofusine-treated mice exhibited significantly reduced kidneys values (1.69±0.15%ID/g vs. 5.55±0.94%ID/g in controls, P <0.001). Dosimetry data revealed very comparable effective tumour doses for the two scaling models applied, of 0.045 and 0.044mSv/MBq. Conclusion: The present study has provided convincing toxicology, biodistribution and dosimetry data for prompt implementation of the freeze-dried kit formulation without or with gelofusine administration in a multicentre clinical trial in MTC patients
Human,climatic and oceanographic influences on the marine environment of Pohnpei, Federated States of Micronesia
No full textFile PB200200 could not be included in folder EFR1. Full data available on disc with print copy held at the University of Waikato Library.Coral reefs and marine resources are culturally, as well as economically, vital to Pohnpei, situated in the Federated States of Micronesia (FSM). Farming and fishing are the main sources of livelihood for most Pohnpeian communities. Pohnpei has eleven Marine Protected Areas (MPAs) where nine are situated in the Pohnpei Island Lagoon and two MPAs on the outer low-lying atolls. Like many other Pacific Island countries Pohnpei is on the verge of creating more MPAs. However, the marine environment continues to be significantly threatened by human and natural influences. The recognised threats are yet to be methodically investigated.
This thesis used a combination of sediment, coral, fish, climatic, and oceanographic data, and focused on the Pohnpei Lagoon, examining a range of natural and human issues in the marine environment both at the local level (focusing on that within the Pohnpei Lagoon) and regional level (focusing on the western Pacific region).
Evidence from historical, archaeological, and modern experience has influenced various marine impacts that have altered the coastline and the marine environment of the Pohnpei Lagoon. Humans have greatly impacted on the coral diversity and fish populations in the Pohnpei Lagoon by over-fishing and contributing to accelerated sediment inputs. My study findings shows that that increased sea surface temperature (SST) caused by El Ni o events is not the only cause of coral bleaching, but also cooling of SST, and other human factors. However, when corals bleach they recover by symbiont shuffling . This is an ingenious way in which corals host one or more varieties of their zooxanthelle (Symbiodinium symbiont clades) that are more tolerant of the stress caused by increased SST and human factors.
The recognised natural climatic variability, particularly the El Ni o/Southern Oscillation (ENSO), may pose a significant threat to the Pohnpei Lagoon. El Ni o events are associated with: a change in trade winds and stronger wind gusts attributed to typhoons; lower rainfall causing drought; a decrease in SST attributed to cooling of the marine environment; increase of salinity in marine estuaries affecting development and recruitment of marine species communities; and a steep fall in sea level exposing corals to other elements. The various on-going human threats and El Ni o-like conditions have caused giant clams (Tridacna gigas) to become extinct, have endangered herbivorous fish populations, and caused coral bleaching by cooling of SST.
Although high SSTs are normally blamed for coral bleaching, the last major bleaching event in Pohnpei (2002) was likely to be due to a reduction in salinity (freshwater runoff and lower sea level), and there has been strong recovery. However, decreasing water temperatures rather than increases of SSTs may contribute to coral bleaching in the Pohnpei Lagoon and the Micronesian region. The Micronesian region appears to have suffered relatively few episodes of regional coral bleaching events. This is due to the Western Pacific Warm Pool (WPWP) where sea surface temperatures exceed 29 C but also where various feedback mechanisms limit the maximum SSTs.
The management aims of Pohnpei's MPAs are to move forward, while still respecting traditional practices. However, a lack of scientific monitoring, technical support and funding restricts our understanding of human and natural influences on the existing MPAs and the Pohnpei Lagoon. With respect to our policy makers the findings of the present research have implications on the future work in Pohnpei's marine environment and for policy makers, to make more-informed decisions before establishing new MPAs.
My key recommendations were: 1.) Integrate coral and fish monitoring during and after El Ni o events to understand El Ni o effects on the Pohnpei environment. 2.) Undertake herbivorous fish investigation into their populations inside and outside the MPAs. 3.) Do not cut down vegetation along coastline areas, as it prevents erosion 4.) Investigate Symbiodinium coral clades in Pohnpei Lagoon and the outer low-lying atolls
