4,011 research outputs found
FT-infrared spectroscopic studies of lymphoma, lymphoid and myeloid leukaemia cell lines
No full textThis paper presents a novel method to characterise spectral differences that
distinguish leukaemia and lymphoma cell lines. This is based on objective
spectral measurements of major cellular biochemical constituents and
multivariate spectral processing. Fourier transform infrared (FT-IR) maps of the
lymphoma, lymphoid and myeloid leukaemia cell samples were obtained using a
Perkin-Elmer Spotlight 300 FT-IR imaging spectrometer. Multivariate statistical
techniques incorporating principal component analysis (PCA) and linear
discriminant analysis (LDA) were used to construct a mathematical model. This
model was validated for reproducibility. Multivariate statistical analysis of
FTIR spectra collected for each cell sample permit a combination of unsupervised
and supervised methods of distinguishing cell line types. This resulted in the
clustering of cell line populations, indicating distinct bio-molecular
differences. Major spectral differences were observed in the 4000 to 800 cm-
1 spectral region. Bands in the averaged spectra for the cell line were assigned
to the major biochemical constituents including; proteins, fatty acids,
carbohydrates and nucleic acids. The combination of FT-IR spectroscopy and
multivariate statistical analysis provides an important insight into the
fundamental spectral differences between the cell lines, which differ according
to the cellular biochemical composition. These spectral differences can serve as
potential biomarkers for the differentiation of leukaemia and lymphoma cells.
Consequently these differences could be used as the basis for developing a
spectral method for the detection and identification of haematological
malignancies
From Canon Road, 900 ft. above river, S.E. to Upper Falls (109 ft. high), Yellowstone Park, U.S.A.
No full textFrom Canon Road, 900 ft. above river, S.E. to Upper Falls (109 ft. high), Yellowstone Park, U.S.A
Electric Peak (11155 ft.), highest elevation in Yellowstone Park, U.S.A.
No full textElectric Peak (11155 ft.), highest elevation in Yellowstone Park, U.S.A
Old Faithful in action (150 ft.), the chief attraction of the Upper Geyser Basin, Yellowstone Park, U.S.A.
No full textOld Faithful in action (150 ft.), the chief attraction of the Upper Geyser Basin, Yellowstone Park, U.S.A
Most famous sight in Yellowstone Park - "Old Faithful" Geyser in action (height, 180 ft. 1,500,000 gallons each eruption), U.S.A., The
No full textMost famous sight in Yellowstone Park - "Old Faithful" Geyser in action (height, 180 ft. 1,500,000 gallons each eruption), U.S.A., Th
FT-IR, FT-Raman, NMR spectra and DFT simulations of 4-(4-fluoro-phenyl)-1H-imidazole
No full textWOS: 000317987400006The FT-IR, FT-Raman and FT-NMR spectra of the compound 4-(4-Fluoro-phenyl)-1H-imidazole (4-FPI) were recorded and analyzed. Density functional method (B3LYP level with the 6-311G(d, p) and 6-311++G(d, p) and cc-pVQZ as basis sets) has been used to compute optimized geometry, vibrational wavenumbers of the 4-FPI. Only one tautomeric form was found most stable by using DFT/B3LYP. The detailed interpretation of the vibrational spectra was carried out with the aid of total energy distribution following the scaled quantum mechanical force field methodology. Potential Energy Surface scan studies has also been carried out by ab initio calculations with the same basis sets.Ahi Evran UniversityAhi Evran UniversityThe author (Y. Erdogdu) would like to thank Ahi Evran University Research Fund for its financial support. We would like to thank the central laboratory of METU (ODTU) for recording FT-Raman spectra, Gazi University Art and Science Faculty Department of Chemistry for recording FT-IR spectra
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[Roundtable Writer's Breakfast, A Sailor's Perspective Series 2 of 2, "My Golden Nuggets: Life Changing Devotions and Meditations" ft. author Ronnie C. King.]
No full textVideo recording from The Black Academy of Arts and Letters recorded during their Roundtable Writer's Breakfast, A Sailor's Perspective Series 2 of 2, "My Golden Nuggets: Life Changing Devotions and Meditations" ft. author and Navy Captain and Chaplain Ronnie C. King. King talks about his time and career with the US Military
[Roundtable Writer's Breakfast, A Sailor's Perspective Series 2 of 2, "My Golden Nuggets: Life Changing Devotions and Meditations" ft. author Ronnie C. King.]
No full textVideo recording from The Black Academy of Arts and Letters recorded during their Roundtable Writer's Breakfast, A Sailor's Perspective Series 2 of 2, "My Golden Nuggets: Life Changing Devotions and Meditations" ft. author and Navy Captain and Chaplain Ronnie C. King. King talks about his time and career with the US Military
The Social Cost-of-Living: Welfare Foundations and Estimation
No full textWe present a new class of social cost-of-living indices and a nonparametric framework for estimating these and other social cost-of- living indices. Common social cost-of-living indices can be understood as aggregator functions of approximations of individual cost-of-living indices. The Consumer Price Index (CPI) is the expenditure-weighted average of first-order approximations of each individual’s cost-of-living index. This is troubling for three reasons. First, it has not been shown to have a welfare economic foundation for the case where agents are heterogeneous (as they clearly are.) Second, it uses an expenditure-weighted average which downweights the experience of poor households relative to rich households. Finally, it uses only first-order approximations of each individual’s cost-of-living index, and thus ignores substitution effects. We propose a “common-scaling” social cost-of-living index, which is defined as the single scaling to everyone’s expenditure which holds social welfare constant across a price change. Our approach has an explicit social welfare foundation and allows us to choose the weights on the costs of rich and poor households. We also give a unique solution for the welfare function for the case where the weights are independent of household expenditure. A first order approximation of our social cost-of- living index nests as special cases commonly used indices such as the CPI. We also provide a nonparametric method for estimating second- order approximations (which account for substitution effects).Inflation, Social cost-of-living, Demand, Average Derivatives
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No full textThe Drosophila protocadherin Fat (Ft) regulates growth, planar cell polarity (PCP) and proximodistal patterning. A key downstream component of Ft signaling is the atypical myosin Dachs (D). Multiple regions of the intracellular domain of Ft have been implicated in regulating growth and PCP but how Ft regulates D is not known. Mutations in Fbxl7, which encodes an F-box protein, result in tissue overgrowth and abnormalities in proximodistal patterning that phenocopy deleting a specific portion of the intracellular domain (ICD) of Ft that regulates both growth and PCP. Fbxl7 binds to this same portion of the Ft ICD, co-localizes with Ft to the proximal edge of cells and regulates the levels and asymmetry of D at the apical membrane. Fbxl7 can also regulate the trafficking of proteins between the apical membrane and intracellular vesicles. Thus Fbxl7 functions in a subset of pathways downstream of Ft and links Ft to D localization
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