1,720,955 research outputs found
Mitochondrial Translocation of EGFR Regulates Mitochondrial Dynamics and Promotes Cancer Metastasis in NSCLC
No full text粒線體功能失常被發現與癌症產生有相關連。在本篇研究中，我們利用同位素相對標記與絕對定量技術（isobaric tag for relative and absolute quantitation, iTRAQ）分析具有不同侵襲能力的肺癌細胞中其粒線體蛋白質的組成有何不同。我們發現上皮生長因子受器（Epidermal growth factor recptor, EGFR）在具有高度侵襲能力的CL1-5細胞粒線體中量較多。另外，我們發現EGF可以使EGFR移至粒線體中並引起粒線體的形態改變分裂。也因此粒線體EGFR促使能量產升上升、細胞運動能力增加，且造成粒線體分布到運動偽足前側。另外，無論EGFR磷酸化與否，EGFR都可以影響粒線體形態。粒線體中EGFR被發現可與粒線體融合蛋白Mfn1交互作用，在mitEGFR表現的細胞中過量表現Mfn1後，可發現Mfn1可以回復mitEGFR所造成細胞特性改變，諸如粒線體形態改變、能量產生與細胞運動能力，發現mitEGFR可能是藉由影響Mfn1聚合而影響粒線體形態。有趣的是，由臨床病人檢體發現，粒線體中EGFR表現與病人存活率呈現負相關，且相較於原位癌部位，在淋巴癌轉移部位之粒線體EGFR表現量相對較多。總結來說，本篇研究發現EGFR可藉由EGF引起的內吞現象轉位到粒線體中，並且造成粒線體形態分裂，而引起能量產生改變、粒線體分布於運動偽足前端，進而使癌細胞運動能力上升。粒線體EGFR的存在可能與癌細胞侵襲相關，且可做為癌症預後因子。Dysfunction of the mitochondria, the versatile cellular organelles, is shown to be related to cancer progression. In the present study, the iTRAQ was exploited to analyze mitochondrial proteomics of lung cancer cell lines with variable migration abilities. We found that EGFR is highly expressed in highly invasive lung cancer cell mitochondria. We demonstrated that the mitochondrial translocation of EGFR by EGF induces mitochondrial fission, and upregulates energy production, causes mitochondrial redistribution in the lamellipodia, and enhances cell motility. Besides, EGFR can still regulate mitochondria dynamics and cell motility, independent of its phosphorylation status. Furthermore, EGFR was found to interact with mitofusion1 (Mfn1), a mitochondrial protein which causes mitochondria fusion by polymerization to regulate mitochondrial dynamics. Overexpressing Mfn1 significantly reversed the phenotypes resulted from mitochondrial translocation of EGFR. Corresponding to the above finding, the EGFR expression in cytosol rather than on the cell surface is reversely correlated to the overall survival of NSCLC patients. Notably, the cytosolic EGFR expression levels in the lymph node-locating tumor cells are higher than that of the paired primary tumor sites. Collectively, our results show that mitochondrial EGFR plays an important role on mitochondrial morphology, energy production and distributions, which further promotes cellular motility. Accordingly, mitochondrial EGFR expression is involved in cancer invasion and can serve as a diagnostic marker for predicting NSCLC malignancy
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
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