8 research outputs found

    Criminal and victim identification based on soft biometrics

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    Criminal and victim identification is always important in forensic investigation. However, it can be a very challenging problem for identifying criminals and victims in digital media when only their non-facial body sites are available in evidence images. These criminals and victims can be masked gunmen, paedophiles, and victims in child pornographic and voyeur images. To solve the above problem, several novel alignment and identification approaches are proposed in this thesis. Firstly, lower leg geometry is proposed as a soft biometric trait for criminal and victim identification. This study provides a foundation for further research based on body geometry. Secondly, leg geometry and hair follicles are proposed to align the androgenic hair patterns in consideration of viewpoint and pose variations, which were ignored by a recent paper suggesting androgenic hair patterns for identification. Experiments on 1,138 high and low resolution images from 283 different legs show that the proposed alignment algorithms provide improvements of 5%-10% on different experimental settings. Thirdly, a new approach is developed to improve the identification of androgenic hair patterns significantly. In the past, it was believed that androgenic hair patterns in low resolution images are not a distinctive biometric trait because of the previous result. A new algorithm, which makes use of leg geometry to align lower leg images, large feature sets (about 60,000 features) extracted through multi-directional gridding systems to increase discriminative power and robustness, the partial least squares (PLS) method to handle imbalanced training data and to perform the multi-grid feature fusion, and scheme generating more positive samples to increase robustness against viewpoint and pose variation, is proposed. Experimental results on 1,493 low resolution leg images with large viewpoint and pose variations from 412 legs demonstrate the proposed multi-grid feature fusion algorithm provides a significant improvement. Fourthly, estimation of soft biometric traits (e.g. height, BMI, age) is proposed. Recent studies demonstrated that soft biometric traits can be effectively estimated through comparative verbal descriptions (e.g. taller and shorter) given by witnesses. The usage of descriptions is extended to non-verbal comparative features extracted from legs for soft biometrics estimation. The proposed algorithm is examined on more than 2000 front and side leg images. The experimental results demonstrate that the proposed algorithm offers comparable performance with the existing methods. Fifthly, the distinctiveness of human skin texture is evaluated by comparing with blood vessels. Recent studies show that blood vessel patterns can be visualized in different body parts. However, their visualization methods have limitations on images with low quality and subjects with high concentration of body fat, high concentration of melanin, and dense hair. In this study, more than 6000 inner forearm and thigh images were collected from a laboratory environment and the Internet with large pose, viewpoint, resolution, and illumination variations. The experimental results indicate the potential use of skin texture for criminal and victim identification.Doctor of Philosophy (SCE

    A further study of low resolution androgenic hair patterns as a soft biometric trait

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    Soft biometric traits such as skin color, tattoos, shoe size, height, and weight have been regularly used for forensic investigation, especially when hard biometric traits, e.g., faces and fingerprints are not available. Recently, a new soft biometric trait, androgenic hair also called body hair, was evaluated. The previous study showed that low resolution androgenic hair patterns have potential for forensic investigation. However, it was believed that they are not a distinctive biometric trait because of the reported accuracy. To explore discriminative information in androgenic hair patterns, in this paper, a new algorithm, which makes use of leg geometry to align lower leg images, large feature sets (about 60,000 features) extracted through multi-directional grid systems to increase discriminative power and robustness, and class-specific partial least squares (PLS) models to utilize the features effectively, is employed. To further enhance the performance of the class-specific PLS models trained on very limited positive samples, one to three images per model in the experiments, and further enhance robustness against viewpoint and pose variations, a scheme is designed to generate more positive samples from a single image. Experimental results on 1493 low resolution leg images with large viewpoint and pose variations from 412 legs demonstrate that low resolution androgenic hair patterns contain rich information and the impression of low discriminative power on androgenic hair is due to the method used in the previous study.MOE (Min. of Education, S’pore)Accepted versio

    Dact Gene Expression Profiles Suggest A Role For This Gene Family In Integrating Wnt And Tgf-β Signaling Pathways During Chicken Limb Development

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    Background: Dact gene family encodes multifunctional proteins that are important modulators of Wnt and TGF-β signaling pathways. Given that these pathways coordinate multiple steps of limb development, we investigated the expression pattern of the two chicken Dact genes (Dact1 and Dact2) from early limb bud up to stages when several tissues are differentiating. Results: During early limb development (HH24-HH30) Dact1 and Dact2 were mainly expressed in the cartilaginous rudiments of the appendicular skeleton and perichondrium, presenting expression profiles related, but distinct. At later stages of development (HH31-HH35), the main sites of Dact1 and Dact2 expression were the developing synovial joints. In this context, Dact1 expression was shown to co-localize with regions enriched in the nuclear β-catenin protein, such as developing joint capsule and interzone. In contrast, Dact2 expression was restricted to the interzone surrounding the domains of bmpR-1b expression, a TGF-β receptor with crucial roles during digit morphogenesis. Additional sites of Dact expression were the developing tendons and digit blastemas. Conclusions: Our data indicate that Dact genes are good candidates to modulate and, possibly, integrate Wnt and TGF-β signaling during limb development, bringing new and interesting perspectives about the roles of Dact molecules in limb birth defects and human diseases. Developmental Dynamics 243:428-439, 2014. © 2013 Wiley Periodicals, Inc.2433428439Akiyama, H., Chaboissier, M.C., Martin, J.F., Schedl, A., de Crombrugghe, B., The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6 (2002) Genes Dev, 16, pp. 2813-2828Alvares, L.E., Winterbottom, F.L., Jorge, E.C., Rodrigues Sobreira, D., Xavier-Neto, J., Schubert, F.R., Dietrich, S., Chicken dapper genes are versatile markers for mesodermal tissues, embryonic muscle stem cells, neural crest cells, and neurogenic placodes (2009) Dev Dyn, 238, pp. 1166-1178Bober, E., Brand-Saberi, B., Ebensperger, C., Wilting, J., Balling, R., Paterson, B.M., Arnold, H.H., Christ, B., Initial steps of myogenesis in somites are independent of influence from axial structures (1994) Development, 120, pp. 3073-3082Brott, B.K., Sokol, S.Y., Frodo proteins: modulators of Wnt signaling in vertebrate development (2005) Differentiation, 73, pp. 323-329Brott, B.K., Sokol, S.Y., A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development (2005) Dev Cell, 8, pp. 703-715Cheyette, B.N., Waxman, J.S., Miller, J.R., Takemaru, K., Sheldahl, L.C., Khlebtsova, N., Fox, E.P., Moon, R.T., Dapper, a Dishevelled-associated antagonist of beta-catenin and JNK signaling, is required for notochord formation (2002) Dev Cell, 2, pp. 449-461Chimal-Monroy, J., Rodriguez-Leon, J., Montero, J.A., Ganan, Y., Macias, D., Merino, R., Hurle, J.M., Analysis of the molecular cascade responsible for mesodermal limb chondrogenesis: Sox genes and BMP signaling (2003) Dev Biol, 257, pp. 292-301DeLise, A.M., Fischer, L., Tuan, R.S., Cellular interactions and signaling in cartilage development (2000) Osteoarthrit Cartil, 8, pp. 309-334Duprez, D., Bell, E.J., Richardson, M.K., Archer, C.W., Wolpert, L., Brickell, P.M., Francis-West, P.H., Overexpression of BMP-2 and BMP-4 alters the size and shape of developing skeletal elements in the chick limb (1996) Mech Dev, 57, pp. 145-157Duprez, D.M., Kostakopoulou, K., Francis-West, P.H., Tickle, C., Brickell, P.M., Activation of Fgf-4 and HoxD gene expression by BMP-2 expressing cells in the developing chick limb (1996) Development, 122, pp. 1821-1828Fischer, L., Boland, G., Tuan, R.S., Wnt signaling during BMP-2 stimulation of mesenchymal chondrogenesis (2002) J Cell Biochem, 84, pp. 816-831Fisher, D.A., Kivimae, S., Hoshino, J., Suriben, R., Martin, P.M., Baxter, N., Cheyette, B.N., Three Dact gene family members are expressed during embryonic development and in the adult brains of mice (2006) Dev Dyn, 235, pp. 2620-2630Francis-West, P.H., Abdelfattah, A., Chen, P., Allen, C., Parish, J., Ladher, R., Allen, S., Archer, C.W., Mechanisms of GDF-5 action during skeletal development (1999) Development, 126, pp. 1305-1315Ganan, Y., Macias, D., Duterque-Coquillaud, M., Ros, M.A., Hurle, J.M., Role of TGF beta s and BMPs as signals controlling the position of the digits and the areas of interdigital cell death in the developing chick limb autopod (1996) Development, 122, pp. 2349-2357Gao, X., Wen, J., Zhang, L., Li, X., Ning, Y., Meng, A., Chen, Y.G., Dapper1 is a nucleocytoplasmic shuttling protein that negatively modulates Wnt signaling in the nucleus (2008) J Biol Chem, 283, pp. 35679-35688Gillhouse, M., Wagner Nyholm, M., Hikasa, H., Sokol, S.Y., Grinblat, Y., Two Frodo/Dapper homologs are expressed in the developing brain and mesoderm of zebrafish (2004) Dev Dyn, 230, pp. 403-409Gloy, J., Hikasa, H., Sokol, S.Y., Frodo interacts with Dishevelled to transduce Wnt signals (2002) Nat Cell Biol, 4, pp. 351-357Goulding, M., Lumsden, A., Paquette, A.J., Regulation of Pax-3 expression in the dermomyotome and its role in muscle development (1994) Development, 120, pp. 957-971Hamburger, V., Hamilton, H.L., A series of normal stages in the development of the chick embryo (1992) Dev Dyn, 195, pp. 231-272. , 1951Hartmann, C., Tabin, C.J., Dual roles of Wnt signaling during chondrogenesis in the chicken limb (2000) Development, 127, pp. 3141-3159Hartmann, C., Tabin, C.J., Wnt-14 plays a pivotal role in inducing synovial joint formation in the developing appendicular skeleton (2001) Cell, 104, pp. 341-351Healy, C., Uwanogho, D., Sharpe, P.T., Expression of the chicken Sox9 gene marks the onset of cartilage differentiation (1996) Ann NY Acad Sci, 785, pp. 261-262Hikasa, H., Sokol, S.Y., The involvement of Frodo in TCF-dependent signaling and neural tissue development (2004) Development, 131, pp. 4725-4734Jia, Y., Yang, Y., Brock, M.V., Zhan, Q., Herman, J.G., Guo, M., Epigenetic regulation of DACT2, a key component of the Wnt signaling pathway in human lung cancer (2012) J Pathol.Jiang, X., Tan, J., Li, J., Kivimae, S., Yang, X., Zhuang, L., Lee, P.L., Yu, Q., DACT3 is an epigenetic regulator of Wnt/beta-catenin signaling in colorectal cancer and is a therapeutic target of histone modifications (2008) Cancer Cell, 13, pp. 529-541Kardon, G., Harfe, B.D., Tabin, C.J., A Tcf4-positive mesodermal population provides a prepattern for vertebrate limb muscle patterning (2003) Dev Cell, 5, pp. 937-944Kawakami, Y., Capdevila, J., Buscher, D., Itoh, T., Rodriguez Esteban, C., Izpisua Belmonte, J.C., WNT signals control FGF-dependent limb initiation and AER induction in the chick embryo (2001) Cell, 104, pp. 891-900Kettunen, P., Kivimae, S., Keshari, P., Klein, O.D., Cheyette, B.N., Luukko, K., Dact1-3 mRNAs exhibit distinct expression domains during tooth development (2010) Gene Expr Patterns, 10, pp. 140-143Kivimae, S., Yang, X.Y., Cheyette, B.N., All Dact (Dapper/Frodo) scaffold proteins dimerize and exhibit conserved interactions with Vangl, Dvl, and serine/threonine kinases (2011) BMC Biochem, 12, p. 33Kulyk, W.M., Upholt, W.B., Kosher, R.A., Fibronectin gene expression during limb cartilage differentiation (1989) Development, 106, pp. 449-455Ladher, R.K., Church, V.L., Allen, S., Robson, L., Abdelfattah, A., Brown, N.A., Hattersley, G., Francis-West, P.H., Cloning and expression of the Wnt antagonists Sfrp-2 and Frzb during chick development (2000) Dev Biol, 218, pp. 183-198Lagathu, C., Christodoulides, C., Virtue, S., Cawthorn, W.P., Franzin, C., Kimber, W.A., Nora, E.D., Sethi, J.K., Dact1, a nutritionally regulated preadipocyte gene, controls adipogenesis by coordinating the Wnt/beta-catenin signaling network (2009) Diabetes, 58, pp. 609-619Lee, W.C., Hough, M.T., Liu, W., Ekiert, R., Lindstrom, N.O., Hohenstein, P., Davies, J.A., Dact2 is expressed in the developing ureteric bud/collecting duct system of the kidney and controls morphogenetic behavior of collecting duct cells (2010) Am J Physiol Renal Physiol, 299, pp. F740-F751Meng, F., Cheng, X., Yang, L., Hou, N., Yang, X., Meng, A., Accelerated re-epithelialization in Dpr2-deficient mice is associated with enhanced response to TGFbeta signaling (2008) J Cell Sci, 121, pp. 2904-2912Merino, R., Ganan, Y., Macias, D., Economides, A.N., Sampath, K.T., Hurle, J.M., Morphogenesis of digits in the avian limb is controlled by FGFs, TGFbetas, and noggin through BMP signaling (1998) Dev Biol, 200, pp. 35-45Merino, R., Macias, D., Ganan, Y., Rodriguez-Leon, J., Economides, A.N., Rodriguez-Esteban, C., Izpisua-Belmonte, J.C., Hurle, J.M., Control of digit formation by activin signalling (1999) Development, 126, pp. 2161-2170Merino, R., Rodriguez-Leon, J., Macias, D., Ganan, Y., Economides, A.N., Hurle, J.M., The BMP antagonist Gremlin regulates outgrowth, chondrogenesis and programmed cell death in the developing limb (1999) Development, 126, pp. 5515-5522Millan, F.A., Denhez, F., Kondaiah, P., Akhurst, R.J., Embryonic gene expression patterns of TGF beta 1, beta 2 and beta 3 suggest different developmental functions in vivo (1991) Development, 111, pp. 131-143Montero, J.A., Lorda-Diez, C.I., Ganan, Y., Macias, D., Hurle, J.M., Activin/TGFbeta and BMP crosstalk determines digit chondrogenesis (2008) Dev Biol, 321, pp. 343-356Moore, M.A., Gotoh, Y., Rafidi, K., Gerstenfeld, L.C., Characterization of a cDNA for chicken osteopontin: expression during bone development, osteoblast differentiation, and tissue distribution (1991) Biochemistry, 30, pp. 2501-2508Moorman, A.F., Houweling, A.C., de Boer, P.A., Christoffels, V.M., Sensitive nonradioactive detection of mRNA in tissue sections: novel application of the whole-mount in situ hybridization protocol (2001) J Histochem Cytochem, 49, pp. 1-8Mootoosamy, R.C., Dietrich, S., Distinct regulatory cascades for head and trunk myogenesis (2002) Development, 129, pp. 573-583Newman, S.A., Bhat, R., Activator-inhibitor dynamics of vertebrate limb pattern formation (2007) Birth Defects Res C Embryo Today, 81, pp. 305-319Riddle, R.D., Ensini, M., Nelson, C., Tsuchida, T., Jessell, T.M., Tabin, C., Induction of the LIM homeobox gene Lmx1 by WNT7a establishes dorsoventral pattern in the vertebrate limb (1995) Cell, 83, pp. 631-640Schweitzer, R., Chyung, J.H., Murtaugh, L.C., Brent, A.E., Rosen, V., Olson, E.N., Lassar, A., Tabin, C.J., Analysis of the tendon cell fate using Scleraxis, a specific marker for tendons and ligaments (2001) Development, 128, pp. 3855-3866Schweitzer, R., Zelzer, E., Volk, T., Connecting muscles to tendons: tendons and musculoskeletal development in flies and vertebrates (2010) Development, 137, pp. 2807-2817Smith, A., Graham, A., Restricting Bmp-4 mediated apoptosis in hindbrain neural crest (2001) Dev Dyn, 220, pp. 276-283Spater, D., Hill, T.P., O'Sullivan, R.J., Gruber, M., Conner, D.A., Hartmann, C., Wnt9a signaling is required for joint integrity and regulation of Ihh during chondrogenesis (2006) Development, 133, pp. 3039-3049Storm, E.E., Kingsley, D.M., GDF5 coordinates bone and joint formation during digit development (1999) Dev Biol, 209, pp. 11-27Suriben, R., Kivimae, S., Fisher, D.A., Moon, R.T., Cheyette, B.N., Posterior malformations in Dact1 mutant mice arise through misregulated Vangl2 at the primitive streak (2009) Nat Genet, 41, pp. 977-985ten Berge, D., Brugmann, S.A., Helms, J.A., Nusse, R., Wnt and FGF signals interact to coordinate growth with cell fate specification during limb development (2008) Development, 135, pp. 3247-3257Teran, E., Branscomb, A.D., Seeling, J.M., Dpr Acts as a molecular switch, inhibiting Wnt signaling when unphosphorylated, but promoting Wnt signaling when phosphorylated by casein kinase Idelta/epsilon (2009) PLoS One, 4, pp. e5522Topol, L., Chen, W., Song, H., Day, T.F., Yang, Y., Sox9 inhibits Wnt signaling by promoting beta-catenin phosphorylation in the nucleus (2009) J Biol Chem, 284, pp. 3323-3333Tylzanowski, P., De Valck, D., Maes, V., Peeters, J., Luyten, F.P., Zfhx1a and Zfhx1b mRNAs have non-overlapping expression domains during chick and mouse midgestation limb development (2003) Gene Expr Patterns, 3, pp. 39-42Vortkamp, A., Lee, K., Lanske, B., Segre, G.V., Kronenberg, H.M., Tabin, C.J., Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein (1996) Science, 273, pp. 613-622Waxman, J.S., Hocking, A.M., Stoick, C.L., Moon, R.T., Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes (2004) Development, 131, pp. 5909-5921Welten, M.C., Verbeek, F.J., Meijer, A.H., Richardson, M.K., Gene expression and digit homology in the chicken embryo wing (2005) Evol Dev, 7, pp. 18-28Widelitz, R.B., Jiang, T.X., Murray, B.A., Chuong, C.M., Adhesion molecules in skeletogenesis: II. Neural cell adhesion molecules mediate precartilaginous mesenchymal condensations and enhance chondrogenesis (1993) J Cell Physiol, 156, pp. 399-411Witte, F., Dokas, J., Neuendorf, F., Mundlos, S., Stricker, S., Comprehensive expression analysis of all Wnt genes and their major secreted antagonists during mouse limb development and cartilage differentiation (2009) Gene Expr Patterns, 9, pp. 215-223Witte, F., Chan, D., Economides, A.N., Mundlos, S., Stricker, S., Receptor tyrosine kinase-like orphan receptor 2 (ROR2) and Indian hedgehog regulate digit outgrowth mediated by the phalanx-forming region (2010), 107, pp. 14211-14216. , Proc Natl Acad Sci USAXi, S., Yang, M., Tao, Y., Xu, H., Shan, J., Inchauste, S., Zhang, M., Schrump, D.S., Cigarette smoke induces C/EBP-beta-mediated activation of miR-31 in normal human respiratory epithelia and lung cancer cells (2010) PLoS One, 5, pp. e13764Yamaguchi, T.P., Bradley, A., McMahon, A.P., Jones, S., A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo (1999) Development, 126, pp. 1211-1223Yang, Y., Niswander, L., Interaction between the signaling molecules WNT7a and SHH during vertebrate limb development: dorsal signals regulate anteroposterior patterning (1995) Cell, 80, pp. 939-947Yang, Y., Topol, L., Lee, H., Wu, J., Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation (2003) Development, 130, pp. 1003-1015Yau, T.O., Chan, C.Y., Chan, K.L., Lee, M.F., Wong, C.M., Fan, S.T., Ng, I.O., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing (2005) Oncogene, 24, pp. 1607-1614Zhang, L., Zhou, H., Su, Y., Sun, Z., Zhang, H., Zhang, Y., Ning, Y., Meng, A., Zebrafish Dpr2 inhibits mesoderm induction by promoting degradation of nodal receptors (2004) Science, 306, pp. 114-117Zhang, L., Gao, X., Wen, J., Ning, Y., Chen, Y.G., Dapper 1 antagonizes Wnt signaling by promoting dishevelled degradation (2006) J Biol Chem, 281, pp. 8607-8612Zou, H., Niswander, L., Requirement for BMP signaling in interdigital apoptosis and scale formation (1996) Science, 272, pp. 738-74

    Molecular Genetics of Dupuytren´s Disease

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    Dupuytren’s disease (DD) is a fibromatosis of connective tissue within the palm of the hands. It is characterised by progressive collagen deposition that leads to hardening and thickening of the connective tissue and results in permanent contraction of affected fingers. The aetiology of Dupuytren’s disease is so far unknown and the pathogenesis is favoured by ageing, genetic predisposition, mechanic trauma and possibly other risk factors. Blood and tissue samples of over 850 German and Swiss DD patients were collected in order to analyse the genetics, gene expression patterns and the in vitro behaviour of disease tissue derived fibroblast in 2-D and 3-D models. A genome wide association study (GWAS) with 565 unrelated DD patients and 1,219 controls was performed. Data for 5,204,451 single-nucleotide polymorphisms (SNPs; 186 cases genotyped with Affymetrix Genome-Wide Human SNP Array 6.0 and 379 cases, 1219 controls genotyped with Axiom CEU 1 Array; data imputed with HapMap CEU reference panel) were analyzed for association with DD. SNP rs2290221 on chromosome 7p14, showed the strongest association signal with a p-value of 2.2x10-10 and odds ratio of 2.13. SNP rs2290221 is located intronic of the genes secreted frizzled-related protein 4 (SFRP4) and ependymin related protein 1 (zebrafish) (EPDR1). In addition an integrative replication study with 2,325 Dutch, English and German DD cases and 11,562 controls was performed. It identified nine different susceptibility loci that showed genome-wide significance. Six of these loci contain genes known to be involved in the Wnt/β-catenin signalling pathway. Again the strongest association was seen for the 7p14 locus. Consistent with GWAS findings, a whole genome expression analysis with 12 DD primary disease tissue samples and 12 normal fascia controls revealed upregulation of the Wnt/β-catenin signalling pathway and also changes in mitochondrial function and oxidative stress response in disease tissue. The Wnt signalling pathway is therefore likely to be a key player in the fibromatosis process observed in DD. Primary disease tissue derived fibroblasts at least in part retained their disease associated characteristics in vitro, they exhibited higher proliferation rates and generated strong contraction forces in 3-D collagen gels, and thus present an excellent model for investigating the mechanisms of DD in the context of aging and aging associated diseases
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