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    Prevention of shoot vitrification of strawberry micropropagated shoots proliferated on liquid media by new antivitrifying agents

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    Micropropagated strawberry (Fragaria × ananassa Duch. ’Kent.’) cultured for 4 wk in liquid proliferation media supported on cellulose plugs (sorbarods) developed a high rate of vitrification. This high percentage of abnormal plantlets was significantly reduced by the inclusion of two antivitrifying agents, EM1 and EM2, to the liquid medium. Virtually no vitrification occurred at 5 g L−1of EM1 or EM2. Shoots multiplied on liquid medium supplemented with 5 g L−1 of either EM1 or EM2 had significantly lower fresh weights and lower multiplication rates than on solidified agar medium (0.7%). EM2 produced better growth and proliferation than EM1 or liquid medium alone and was easier to use than EM1. Key words: Vitrification, antivitrifying agent, liquid medium </jats:p

    Changes in ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activities and <sup>14</sup>CO<sub>2</sub> fixation during the rooting of strawberry shoots in vitro

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    The potential for carbon fixation was investigated in in vitro strawberry (Fragaria × ananassa Duch. Kent) shoots 5, 10 and 28 d after transfer to a rooting medium. The activities of ribulose-1,5-bisphosphate carboxylase (Rubisco) and phosphoenolpyruvate carboxylase (PEPC) and the time course of 14CO2 fixation were investigated. Five days after transfer, Rubisco activity was low but was increased two-fold after 28 d. In contrast PEPC activity was highest at 5 d and declined to about 0.4-fold by day 28. The rate of 14CO2 fixation was similar at 5, 10 and 28 d after transfer. However, a more rapid incorporation of 14CO2 into amino acids was observed at 5 than at 10 or 28 d after transfer. These results suggest that strawberry shoots undergo a progressive transition from heterotrophic to autotrophic carbon fixation during their rooting and that PEPC plays an important role in sustaining carbon fixation and amino acid synthesis during the first few days after their transfer to rooting medium. Key words: In vitro culture, phosphoenolpyruvate carboxylase, ribulose-1,5-bisphosphate carboxylase </jats:p

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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