1,215 research outputs found
Replication data for Positive Set of lipidomic data of "PONE-D-24-15462 Untargeted lipidomics reveals unique lipid signatures of extracellular vesicles from porcine colostrum and milk"
No full textThis set of data presents the positive mass spectrometry data. Details can be found in the manuscript. PONE-D-24-15462
Untargeted lipidomics reveals unique lipid signatures of extracellular vesicles from
porcine colostrum and milk.
Rafaela Furioso Ferreira, Morteza H. Ghaffari, Fabricio Ceciliani, Manuela Fontana, Donatella Caruso, Matteo Audano, Giovanni Savoini, Alessandro Agazzi, Vladimir Mrljak, and Helga Sauerwei
Replication data for Negative Set of lipidomic data of "PONE-D-24-15462 Untargeted lipidomics reveals unique lipid signatures of extracellular vesicles from porcine colostrum and milk"
No full textThis set of data presents the negative mass spectrometry data. Details can be found in the manuscript. PONE-D-24-15462
Untargeted lipidomics reveals unique lipid signatures of extracellular vesicles from
porcine colostrum and milk.
Rafaela Furioso Ferreira, Morteza H. Ghaffari, Fabricio Ceciliani, Manuela Fontana, Donatella Caruso, Matteo Audano, Giovanni Savoini, Alessandro Agazzi, Vladimir Mrljak, and Helga Sauerwei
Veterinary Research Communications, Editor of the Special Issue: Clinical Practice Updates in Veterinary Hospitals
No full textThe aim of this special issue is to provide readers with the latest developments in the field of applied veterinary clinical sciences. This collection focuses on the updates and advancements in daily clinical practices conducted in veterinary hospitals. Scientific articles in this issue will revolve around the management of clinical cases or case series, which will be critically analyzed and discussed in the context of existing scientific literature. Additionally, clinical research activities that have direct implications for veterinary practice will also be considered. Contributions can encompass various aspects of veterinary clinical specialties, across both small and large domestic animals, as well as studies involving unconventional and wild animals including one of the following topics are welcomed: • Internal Medicine • Surgery • Clinical Pathology • Diagnostic Imaging • Reproduction • Emergency • Intensive Care Interdisciplinary reports from interns, PhD students, residents, researchers, as well as other academic and non-academic professionals, are highly encouraged. Original papers and literature reviews, in regular or short article format, will be considered for publication. Prospective authors should send an expression of interest (title and abstract) to the Guest Editors
Veterinary Research Communications, Editor of the Special Issue: Clinical Practice Updates in Veterinary Hospitals
No full textThe aim of this special issue is to provide readers with the latest developments in the field of applied veterinary clinical sciences. This collection focuses on the updates and advancements in daily clinical practices conducted in veterinary hospitals. Scientific articles in this issue will revolve around the management of clinical cases or case series, which will be critically analyzed and discussed in the context of existing scientific literature. Additionally, clinical research activities that have direct implications for veterinary practice will also be considered.
Contributions can encompass various aspects of veterinary clinical specialties, across both small and large domestic animals, as well as studies involving unconventional and wild animals including one of the following topics are welcomed:
• Internal Medicine
• Surgery
• Clinical Pathology
• Diagnostic Imaging
• Reproduction
• Emergency
• Intensive Care
Interdisciplinary reports from interns, PhD students, residents, researchers, as well as other academic and non-academic professionals, are highly encouraged. Original papers and literature reviews, in regular or short article format, will be considered for publication.
Prospective authors should send an expression of interest (title and abstract) to the Guest Editors
MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock
No full textMicroRNAs (miRNAs) are small and highly conserved non-coding RNA molecules that orchestrate a wide range of biological processes through the post-transcriptional regulation of gene expression. An intriguing aspect in identifying these molecules as biomarkers is derived from their role in cell-to-cell communication, their active secretion from cells into the extracellular environment, their high stability in body fluids, and their ease of collection. All these features confer on miRNAs the potential to become a non-invasive tool to score animal welfare. There is growing interest in the importance of miRNAs as biomarkers for assessing the welfare of livestock during metabolic, environmental, and management stress, particularly in ruminants, pigs, and poultry. This review provides an overview of the current knowledge regarding the potential use of tissue and/or circulating miRNAs as biomarkers for the assessment of the health and welfare status in these livestock species
sj-pdf-1-vet-10.1177_03009858221128922 – Supplemental material for Salivary miR-21 is a potential biomarker for canine mast cell tumors
No full textSupplemental material, sj-pdf-1-vet-10.1177_03009858221128922 for Salivary miR-21 is a potential biomarker for canine mast cell tumors by Valentina Zamarian, Damiano Stefanello, Roberta Ferrari, Lavinia E. Chiti, Valeria Grieco, Emanuela DallaCosta, Fabrizio Ceciliani and Cristina Lecchi in Veterinary Pathology</p
Supplemental Material, DS1_VET_10.1177_0300985819868646 - MicroRNA Expression in Formalin-Fixed, Paraffin-Embedded Samples of Canine Cutaneous and Oral Melanoma by RT-qPCR
No full textSupplemental Material, DS1_VET_10.1177_0300985819868646 for MicroRNA Expression in Formalin-Fixed, Paraffin-Embedded Samples of Canine Cutaneous and Oral Melanoma by RT-qPCR by Valentina Zamarian, Carlotta Catozzi, Lorenzo Ressel, Riccardo Finotello, Fabrizio Ceciliani, Miguel Vilafranca, Jaume Altimira and Cristina Lecchi in Veterinary Pathology</p
Production and characterization of a polyclonal antibody against the bovine prion-like doppel protein
No full textPRODUCTION AND CHARACTERIZATION OF A POLYCLONAL ANTIBODY AGAINST THE BOVINE PRION-LIKE DOPPEL PROTEIN
Marco Rondena, Fabrizio Ceciliani, Chiara Bazzocchi, Saverio Paltrinieri
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria – Milano
AIM OF THE STUDY: Doppel (Dpl) is a prion-like protein recently identified in mammalian. The sequence is notably homologous to that of PrP. Dpl is associated to neurodegeneration when ectopically expressed in transgenic mouse. The aim of the study is to raise and characterize a polyclonal antibody (pAb) against bovine Dpl to study the tissue distribution of this protein. MATERIAL AND METHODS. A synthetic peptide spanning from residues 67 to 81 of bovine Dpl sequence was synthetized. Using this peptide, a pAb (Dpl 67-81), was raised in rabbit and purified by means of Protein A affinity chromatography. In order to test the specificity of Dpl 67-81, bovine Dpl was cloned and overexpressed in E.coli in the expression vector PQE30 and purified following methal chelate affinity and bovine PrP was purified by means of cation-exchange chromatography and cobalt-chelate affinity chromatography. Homogenate of tissues were tested for the presence of Dpl after SDS-PAGE and Western Blotting. Blots were visualized using ECL. Immunohistochemistry on cryostatic sections using the avidin-biotin complex methods was performed. RESULTS: Dpl 67-81 strongly react against rDpl (titre =1:20000) and not against bovine PrP. Dpl was detected in testis, spleen, limphnodes by both immunoblotting and immunohistochemistry. In particular endothelial and stromal cells showed a granular cytoplasmic positivity. CONCLUSIONS: Dpl 67-81 can detect the expression of Dpl in tissues using both Western Blotting and immunohistochemistry. Moreover, it does not crossreact with PrP, and therefore it could be also used in experiments of functional blocking of Dpl in order to study its function and its relationship with PrP
The immune functions of alpha1 acid glycoprotein
No full textα1-acid glycoprotein (orosomucoid, AGP) is an Acute Phase Protein produced by liver and peripheral tissues in response to systemic reaction to inflammation. AGP functions have been studied mostly in human, cattle and fish, although the protein has been also found in many mammalian species and birds. AGP fulfils at least two set of functions, which are apparently different from each other but in fact intimately linked. On one hand, AGP is an immunomodulatory protein. On the other hand, AGP is one of the most important binding proteins in plasma and, beside modulating pharmacokinetics and pharmacodynamics of many drugs, it is also able to bind and transport several endogen ligands related to inflammation. The focus of this review is the immunomodulatory activity of AGP. This protein regulates every single event related to inflammation, including binding of pathogens and modulating white blood cells activity throughout the entire leukocyte attacking sequence. The regulation of AGP activity is complex: the inflammation induces not only an increase in AGP serum concentration, but also a qualitative change in its carbohydrate moiety, generating a multitude of glycoforms, each of them with different, and sometimes opposite and contradictory, activities. We also present the most recent findings about the relationship between AGP and adipose tissue: AGP interacts with leptin receptor, and, given its immunomodulatory function, it may be included among the potential players in the field of immunometabolism
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