322,862 research outputs found
Segregation of heteroplasmic length variants in HVI region of mitochondrial DNA by size-based separation method.
No full textPCR detection of Y specific amelogenin sequence in Turner syndrome by modified primer set.
No full textImmunolocalisation of desmoglein-1 in equine muzzle skin
No full textDesmogleins (Dsgs) are glycoproteins of the cadherin family that
allow the formation of desmosomes via a Ca2+-dependent
homophilic interaction. Desmosomes are cell-cell adhesion
systems that contribute to the mechanical integrity of epidermis by
linking to keratin intermediate filaments and, therefore, connecting
the cytoskeleton of adjacent keratinocytes (Cozzani et al. 1994).
On light microscopy, desmosomes appear as intercellular bridges.
On electron microscopy they consist of symmetrical disc-like
structures, which link the intermediate filaments system within
cells to the plasma membrane and to adjacent cells (North et al.
1999). Four isoforms of Dsgs have been described in human
(Whittock 2003), one in bovine (Koch et al. 1990; Puttagunta et al.
1994), 2 in canine (Muller et al. 2000; Aoki et al. 2002), 6 in
murine (Whittock 2003) and, recently, one in porcine (Nishifuji et
al. 2005) species. The production of autoantibodies against
desmoglein-1 (Dsg-1) leads to the loss of epidermal cell-cell
adhesion responsible for pustular lesions in the life-threatening
skin disease pemphigus foliaceus (PF) (McMillan and Shimizu
2001). The pathogenesis of PF has been intensively investigated in
man and the molecular characteristics of human Dsgs analysed
(Mahoney et al. 1999). In the canine species Dsg-1 has been
characterised and the hypothesis that this glycoprotein is the target
antigen in PF is strongly supported (Suter et al. 1993; Iwasaki et
al. 1997; Steeves et al. 2002). However, contrasting evidence
concerning a minor role of Dsg-1 in the pathogenesis of canine PF
has been reported (Olivry et al. 2006).
The first case of equine PF was reported by Barnick and
Gutzeit (1891) and since 1980 only a few such reports have been
documented in this species (Peter et al. 1981; Messer and Knight
1982; Rothwell et al. 1985; Laing et al. 1992; Wohlsein et al.
1994; Stahli et al. 2005). Recently, Vandenabeele et al. (2004) and
Zabel et al. (2005) reviewed equine PF.
In horses diagnosis is based mainly on clinical and
histopathological features, which strictly resemble those described
in other species and on the response to immunosuppressive drugs
(Vandenabeele et al. 2004; Zabel et al., 2005). Nevertheless, the
presence of Dsg-1 in equine epidermis has never been
documented and whether this transmembrane protein is targeted
by circulating antibodies in horses with PF is still unknown.
This study documents the presence of a pericellular antigen in
horse muzzle skin by immunohistochemistry using 2
commercially available monoclonal antibodies raised against
bovine Dsg-1-2 and human Dsg-1 and the presence of 2 bands by
immunoblotting analyses using anti-human-Dsg-1
ABO blood group genotyping of multiple single nucleotide polymorphisms using SnaPshot.
No full textA novel reproducible strategy to type the ABO blood group system by the minisequencing of five nucleotide positions using SNaPshot (Applied Biosystem, USA) is described. This new technique has been successfully applied on 30 blood donors and the results agree with the known serologically based predicted genotypes. (C) 2003 Elsevier B.V. All rights reserved
Genetic polymorphism of the new PowerPlex® ESI 17 system in a Tibetan population from Dharamsala (India)
No full textAlleles frequencies for the 17 short tandem repeats (STRs) loci included in the PowerPlex® ESI 17 kit were investigated in a sample of 80 unrelated individuals from a Tibetan ethnic group living in Dharamsala in the state of Himachal Pradesh (Northern India). Due to the relative novelty of this kit, including the 5 new ENFSI recommended loci (D22S1045, D2S441, D10S1248, D1S1656, D12S391) together with SE33 locus, no information regarding all these new loci is available for the Tibetan population. The aims of the present study were to determine the genetic polymorphism of forensic validated markers and to increase the ethnical population database
Analysis of aged seminal stains by current forensic DNA approach
No full text‘Cold’ cases involving sexual assault can be solved decades after investigations analysing DNA from stored evidences, collected when current forensic method were not developed. To evaluate the influence of time and storage conditions on presumptive test and DNA analysis, three aged seminal stains date back to the first half of the ‘900, recovered during restoration work of the Institute of Legal Medicine of the University of Bologna, were analysed by the semenogelin test, microscopic identification of spermatozoa, autosomal, Y-chromosome and X chromosome STRs, and mitochondrial DNA analysis. All samples displayed a weak positive reaction for semenogelin test and the sperm heads were clearly identified. The number of detectable autosomal and Y-STRs was strongly correlated to the DNA degradation index, except for one specimen showing a high degradation index but enough DNA to obtain a composite profile. STRs included in the Minifiler kit and smaller STRs in ESX System were reproducible in all analysed specimens. Aged seminal stains may be successfully analysed by current forensic methods, even if the selection of appropriate amplicons size based on DNA amount and its degradation index is mandatory to predict the success probability in casework
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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