3,387 research outputs found
Isomaltulose: The Next Sweetener, A Quick Review
No full textThe social behavior of new generations consume highly processed foods and high levels of fat and sugar, such as glucose or high-fructose syrup (corn syrup), commonly used in the manufacture of hard candies, have generated a growing increase in cases of diabetes and nutrition problems. In this context, we present the isomaltulose as a substitute potential for sweeteners, due to its property of maintaining stable glycemic levels, dosing energy to the organism in a long-term way, promoting the fat oxidation and it is a noncariogenic food. There is just one company that distributes isomaltulose in an industry level, under the name of Palatinose™ (Beneo-Palatinti, Sudzücker Group, Mannheim, Germany) and already incorporated isomaltulose products in the food processing as beverage, bakery, energy drinks, dry-powder drinks, chewing gum, hard candies, and others. But it is necessary to produce quantities of isomaltulose that will supply the demand of the food industry. The latest advances in biotechnology provide tools to efficiently produce 278any product that may result from the metabolism of micro-organisms. But first, it is important to know the global aspects of the enzyme, the reaction that catalyzes and its products to determine their chemical and biological parameters. This work aims to make a quick review of all these aspects that allow us to understand and start the biotechnological path for the design and development of future foods based on isomaltulose.Fil: Bracho Oliveros, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Ramírez Gutiérrez, Andrea Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila; MéxicoFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
Episode 35: Alexis Castellanos, Author of “Isla to Island”, and Her Panel Presentation during the Operación Pedro Pan Two-Day Event
No full textIn Part 1 of “Operación Pedro Pan: The Voices and Stories of Cuba’s Child Exodus—A Knights HistoryCast Mini-Series,” the Department of History’s Sebastian Garcia talked with Alexis Castellanos, an author, illustrator, graphic novelist, and a panelist at the esteemed, conspicuous, and powerful “Operación Pedro Pan: Honoring the Cultural, Historical Legacy of Cuba’s Child Exodus” Two-Day Program that Florida Humanities, UCF’s Department of English and Department of Modern Languages and Literatures sponsored (see https://cah.ucf.edu/pedro-pan/ for more details on sponsors and the program in general).
Sebastian structured this specific episode on Alexis Castellanos’ Isla to Island, a wordless graphic novel grounded by her personal family history and the history of Operación Pedro Pan (Operation Peter Pan). By analyzing such a historic event through the medium of fiction, Sebastian argued that this is one of the most unique Knights HistoryCast episodes of all time. Naturally, their conversation expanded to what she talked about during her panel presentation in Panel One, Day 1 of the event that featured “internationally renowned scholars that discussed the political, historical, and cultural legacy of Operación Pedro Pan (1960-1962).” (https://cah.ucf.edu/pedro-pan/)
To purchase Isla to Island (strongly recommend), check out: https://islatoisland.com/.
To find out more about Alexis and her professional work, check out her website at https://alexiscastellanos.com/https://stars.library.ucf.edu/knightshistorycast/1034/thumbnail.jp
Kinetic modelling of thermal inactivation of a keratinase from Purpureocillium lilacinum LPSC # 876 and the influence of some additives on its thermal stability
No full textThermal inactivation of a keratinase produced by Purpureocillium lilacinum LPSC #876 was kinetically investigated using several enzyme inactivation models at the temperature range of 50–65 °C. Among the models studied, the Weibull distribution was the best model that describes the residual activity of P. lilacinum keratinase after heat treatment over the selected temperatures. The stabilising effect of metal ions (Ca2+ or Mg2+, 5 mmol l−1 ) or polyols (propylene glycol and glycerol, 10 % v/v) was investigated, showing that the presence of Ca2+ increases the enzyme stability significantly. Conforming to the increased Ca2+ concentration, thermal stability of the enzyme also increased, with 10 mM of Ca2+ being the concentration of metal in which the enzyme retained 100 % of its original activity after being incubated for 1 h at 55 °C. The effects of temperature on Weibull equation parameters and on the characteristics of the inactivation curves were evaluated. In the absence of any additives (control), the reliable time (tR) of the keratinase, analogous to D value, ranged from 484.16 to 63.67 min, while in the presence of Ca2+ the tR values ranged from 6,221 to 414.95 min at 50– 65 °C. P. lilacinum keratinase is a potentially useful biocatalyst, and therefore, kinetic modelling of thermal inactivation addresses an important topic for its application in various industrial processes.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentin
Immobilization of a keratinolytic protease from Purpureocillium lilacinum on genipin activated-chitosan beads
No full textKeratinase from Purpureocillium lilacinum LPSC # 876 was immobilized on chitosan beads using two different cross-linking agents: glutaraldehyde and genipin. For its immobilization certain parameters were optimized such as cross-linker concentration, activation time and activation temperature. Under optimum conditions, enzyme immobilization resulted to be 96 and 92.8% for glutaraldehyde and genipin, respectively, with an activity recovery reaching up to 81% when genipin was used. The immobilized keratinase showed better thermal and pH stabilities compared to the soluble form, retaining more than 85% of its activity at pH 11 and 74% at 50 °C after 1 h of incubation. The residual activity of immobilized keratinase remained more than 60% of its initial value after five hydrolytic cycles. The results in this study support that glutaraldehyde could be replaced by genipin as an alternative cross-linking eco-friendly agent for enzyme immobilization.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; ArgentinaFil: Contreras Esquivel, Juan Carlos. Universidad Autónoma de Coahuila; MéxicoFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentin
Protopectinasa-se de Geotrichum klebahnii: Estudios de adsorción y capacidad de solubilización de pectina
No full textProtopectinasa-SE (PPasa-SE) es una poligalacturonasa producida por Geotrichum klebahnii con capacidad de liberar pectina por hidrólisis de protopectina. La reacción de liberación de pectina responde a un sistema de catálisis heterogenea, donde la interacción enzima-protopectina y la liberación de pectina, son objetivos de esta investigación. La interacción enzima-protopectina resultó dependiente del tamaño y estructura de las partículas del sustrato, así como de la naturaleza de la solución amortiguadora. Para partículas pequeñas la cinética de adsorción siguió un comportamiento definido por la isoterma de Langmuir. La reacción respondió al modelo cinético de Michaelis-Menten, con valores de Km y Vmax de 30.2 g/L y 57.3 g/L.h. Los mejores resultados en cuanto a la adsorción de la enzima y la solubilización de pectina fueron obtenidos con una solución amortiguadora de citrato y citrato-fosfato. Este trabajo constituye un primer reporte en cuanto a la solubilización de pectina que involucra un modelo de unión al sustrato asociado en sistemas pectinasa-protopectinasa.Protopectinase SE (PPase-SE) is a polygalacturonase produced by Geotrichum klebahnii with the capacity to liberate pectin through protopectin hydrolysis. The protopectin cleavage is a typical heterogeneous-catalysis reaction whose interaction between the enzyme and the protopectin substrate from lemon albedo along with the release of the pectin-reaction product were the objectives of this investigation. The interaction between PPase-SE and protopectin depended on the particle size and the structure of the substrate as well as on the nature of the buffer. The adsorption kinetics follows, for small particles, a Langmuir isotherm pattern. The reaction exhibited Michaelis-Menten kinetics, giving respective apparent-Km and Vmax values of 30.2 g/l and 57.3 g/l.h. The better results in enzyme adsorption and pectin releasing were obtained with citrate and citrate-phosphate buffers. This report constitutes the first investigation in pectin solubilization involving a model for the substrate-binding mechanism within the pectinase-protopectinase system.Fil: Zapata Zapata, Arley David. Universidad Nacional de Colombia; ColombiaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
Control of agitation rate and aeration for enhanced polygalacturonase production in submerged fermentation by Aspergillus sojae using agro-industrial wastes
No full textBACKGROUND: The koji mold Aspergillus sojae, an industrially important microorganism, can produce high levels of pectinases utilizing agro-industrial wastes. This study introduces apricot and peach pomace, two agro-industrial wastes barely considered as raw material for the generation of value-added products, and focuses on its utilization together with orange peel for polygalacturonase production in submerged cultures using A. sojae. RESULTS: A Doehlert response surface methodology design conducted in shake flasks and applied individually with these three by-products led to 60–80 U mL−1 polygalacturonase activity. In bioreactor studies performed with a mixture of apricot pomace and orange peel, by fixing stirrer speed to 600 rpm and cascading airflow to the dissolved oxygen tension up to 1.7 vvm, oxygen limitation problems were overcome and polygalacturonase activity values of 380 U mL−1 were achieved. CONCLUSION: A simple and efficient strategy to minimize oxygen limitation with the lowest possible shear stress is provided for stirred-tank bioreactors working with highly viscous broths, so as to ultimately enhance microbial enzyme production. The polygalacturonase activity yields obtained in our study are among the highest reported in the literature.Fil: Fratebianchi de la Parra, Dante. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Crespo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Tari, Canan. Izmir Institute of Technology; TurquíaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin
"Cronica der Turckey" Sebastian Franck's Translation of the "Tractatus de Moribus, Condicionibus et Nequitia Turcorum" by Georgius de Hungaria
No full textThe Tractatus de moribus, condicionibus et nequitia Turcorum is one of the most important first-hand accounts of life in fifteenth-century Turkey known to modern scholarship. It is the work of a Christian former slave of the Turks, writing after his return to the West. Although the author does not name himself, he can be identified as a
Dominican priest, Georgius de Hungaria, who died in Rome in 1502. His Tractatus is conceived as a work of anti-Islamic polemic, yet it contains a surprisingly unbiased appraisal of Turkish customs.
First printed c.1480 when European apprehension in the face of Ottoman expansion was at its height, the Tractatus was reprinted in numerous editions, and was widely used as a
source by other authors. Luther edited the text in 1530, using the positive account of Turkish customs and religious observance as a weapon in his polemic against the Roman
Catholic Church: if heathens could perform such exemplary works, who could fail to doubt the efficacy of works as a means of salvation?
Sebastian Franck in his German translation of the Tractatus went further: replacing Georgius' commentary with his own, he used the text to attack institutional religion as a
whole and to promote his concept of a non-dogmatic, spiritual Church of individuals united with each other only through their union with God -a Church which was not closed to Moslems or members of any other creed. This translation or adaptation, the Cronica der Türckey, marks Franck's decisive break with the Lutheran cause and the beginning of his lonely path as a 'spiritual individualist'. Franck reworked his translation of the Tractatus for his major geographical work, the Weltbuch of 1534.
This thesis concerns itself primarily with Franck's Cronica, providing the first modern critical edition of this text, in a near-diplomatic transcription with an extensive glossary. The thesis also includes transcriptions of the Tractatus; of Türckei, an anonymous translation of the Tractatus, and of relevant additional material from Franck's Weltbuch. None of these texts has been published in full in a modern edition.
In the Introduction Franck's Cronica is compared in detail with the Tractatus, highlighting the changes that occur in translation; the character and the significance of these changes are then discussed. It is established that Franck, whilst being unwilling to reverse any of Georgius' value judgements on Islam and Turkish culture, is highly selective in his choice of material for translation, and frequently gives the text new nuances and adds his own
comment. The question of the Tractatus' influence on Franck's further development as a writer and thinker is also raised.
The investigation then turns to Franck's use of the Tractatus material in his Weltbuch. His eclecticism becomes apparent in this text, in which Georgius' account is juxtaposed - but not synthesised - with material from other sources, often of lesser veracity and greater anti-Islamic bias. Franck's distortion of the Tractatus material to suit his own line of argument is clearly discernible: from the unique phenomenon presented in the Tractatus the Turks
become one more example of the general human tendency to externalise and dogmatise faith.
In addition, the transmission of Cronica and Türckei is examined, and the relationship between these two translations is clarified: Franck certainly used Türckei in writing his Cronica, but is unlikely to be the author of the anonymous work
Evaluation of agro-industrial wastes, their state and mixing ratio for maximum polygalacturonase and biomass production in submerged fermentation
No full textThe potential of important agro-industrial wastes, apple pomace (AP) and orange peel (OP) as C sources, was investigated in the maximization of polygalacturonase (PG), an industrially significant enzyme, using an industrially important microorganism Aspergillus sojae. Factors such as various hydrolysis forms of the C sources (hydrolysed-AP, non-hydrolysed-AP, hydrolysed-AP + OP, non-hydrolysed-AP + OP) and N sources (ammonium sulphate and urea), and incubation time (4, 6, and 8 days) were screened. It was observed that maximum PG activity was achieved at a combination of non-hydrolysed-AP + OP and ammonium sulphate with eight days of incubation. For the pre-optimization study, ammonium sulphate concentration and the mixing ratios of AP + OP at different total C concentrations (9, 15, 21 g l−1) were evaluated. The optimum conditions for the maximum PG production (144.96 U ml−1) was found as 21 g l−1 total carbohydrate concentration totally coming from OP at 15 g l−1 ammonium sulphate concentration. On the other hand, 3:1 mixing ratio of OP + AP at 11.50 g l−1 ammonium sulphate concentration also resulted in a considerable PG activity (115.73 U ml−1). These results demonstrated that AP can be evaluated as an additional C source to OP for PG production, which in turn both can be alternative solutions for the elimination of the waste accumulation in the food industry with economical returns.Fil: Göğüş, Nihan. Izmir Institute of Technology; TurquíaFil: Evcan, Ezgi. Izmir Institute of Technology; TurquíaFil: Tari, Canan. Izmir Institute of Technology; TurquíaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentin
Enzymatic hydrolysis of gelatin layers of X-ray films and release of silver particles using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876
No full textEnzymatic decomposition of gelatin layers on used X-ray films and repeated utilization of the enzyme for potential application in silver recovery were investigated using keratinolytic serine proteases from Purpureocillium lilacinum LPS # 876. At pH 9.0, the enzymatic reaction was enhanced by the increase of enzyme concentration or by the increase of the temperature up to 60oC. Under the conditions of 6.9 U/ml, 60oC, and pH 9.0, hydrolysis of the gelatin layers and the resulting release of silver particles were achieved within 6 min. The protective effect of polyols against thermal denaturation was investigated. The presence of glycerol and propylene glycol increased enzyme stability. When the reusability of the enzyme for gelatin hydrolysis was tested, it could be seen that it could be effectively reused for more cycles when glycerol was added, compared with the enzyme without protective agents. The results of these repeated treatments suggested that a continuous process of recycling silver from used X-ray is feasible. Keeping in mind that recycling is (at the present time) needed and imperative, it can be remarked that, in this research, three wastes were successfully used: hair waste in order to produce serine proteases; glycerol in order to enhance enzyme thermal stability; and used Xray films in order to recover silver and PET films.Fil: Cavello, Ivana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Hours, Roque Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentin
2. A Human Being to Be Remembered | The 2024 UCF VLP Podcast Series
No full textIn Episode Two, Andrew Carroll’s herculean efforts to seek and collect over 210,000 war letters—that span since the American Revolution—demonstrate how others are as seriously committed to preserving the legacies of the men and women who served and fought for their country. Andrew shares several letters from his impressive collection, allowing us to be as close as possible to the Veterans—engaging with their own words, thoughts, and emotions. Indeed, themes central to UCF VLP are often exemplified in the 210,000 war letters Andrew has preserved for over twenty-five years.
Andrew Carroll is an award-winning historian and author and is the founder and director of the Center for American War Letters at Chapman University. Andrew was the second keynote speaker invited to share his extraordinary work during the 2024 UCF VLP Institute.
This episode was directed, produced, written, edited, and hosted by Sebastian Garcia and featured Andrew Carroll.
Executive Producers: Sebastian Garcia and Dr. Amelia Lyons.
Music: “Honor and Glory” and “Real Heroes” by SergePavkinMusic (Pixabay)
Podcast Cover Artwork: Sebastian Garcia
The 2024 UCF VLP Podcast Series is brought to you by the UCF History Department Podcast Network and UCF’s Veterans Legacy Program—a partnership with the U.S. Department of Veterans Affairs’ National Cemetery Administration.https://stars.library.ucf.edu/knightshistorycast/1051/thumbnail.jp
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