2 research outputs found
Descrição de situações: um ensaio em epistemologia contextualista
No full textThis book symposium comprises a précis of Nuno Venturinha’s Description of Situations: An Essay in Contextualist Epistemology (Springer, 2018) together with four critical commentaries on different aspects of the book by Marcelo Carvalho, João Vergílio Gallerani Cuter, Marcos Silva and Darlei Dall’Agnol, and the author’s replies.Este simposio sobre libros comprende un resumen de la Descripción de situaciones: un ensayo de epistemología contextualista de Nuno Venturinha (Springer, 2018) junto con cuatro comentarios críticos sobre diferentes aspectos del libro de Marcelo Carvalho, João Vergílio Gallerani Cuter, Marcos Silva y Darlei Dall'Agnol y las respuestas del autor.O simpósio deste livro é composto por um resumo do livro Description of Situations: An Essay in Contextualist Epistemology (Springer, 2018) de Nuno Venturinha juntamente com quatro comentários críticos sobre diferentes aspectos do livro de Marcelo Carvalho, João Vergílio Gallerani Cuter, Marcos Silva e Darlei Dallgnol , e as respostas do autor
Fructose 1,6-Bisphosphate aldolase, a novel immunogenic surface protein on listeria species
No full textListeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (FBA) class II in Listeria species is the antigen target of the previously described mAb-3F8. Western and dot blot assays confirmed that the mAb-3F8 could distinguish all tested Listeria species from close-related bacteria. Localization studies indicated that FBA is present in every fraction of Listeria cells, including supernatant and the cell wall, setting Listeria spp. as one of the few bacteria described to have this protein on their cell surface. Epitope mapping using ORFeome display and a peptide membrane revealed a 14-amino acid peptide as the potential mAb-3F8 epitope. The target epitope in FBA allowed distinguishing Listeria spp. from closely-related bacteria, and was identified as part of the active site in the dimeric enzyme. However, its function in cell surface seems not to be host cell adhesion-related. Western and dot blot assays further demonstrated that mAb-3F8 together with anti-InlA mAb-2D12 could differentiate pathogenic from non-pathogenic Listeria isolated from artificially contaminated cheese. In summary, we report FBA as a novel immunogenic surface target useful for the detection of Listeria genus. © 2016 Mendonça et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
