1,721,693 research outputs found
Carter (Michael). George Orwell and the problem of Authentic Existence.
No full textD'Haen Theo. Carter (Michael). George Orwell and the problem of Authentic Existence. . In: Revue belge de philologie et d'histoire, tome 64, fasc. 3, 1986. Langues et littératures modernes - Moderne taal- en letterkunde. pp. 658-661
Carter (Michael). George Orwell and the problem of Authentic Existence.
No full textD'Haen Theo. Carter (Michael). George Orwell and the problem of Authentic Existence. . In: Revue belge de philologie et d'histoire, tome 64, fasc. 3, 1986. Langues et littératures modernes - Moderne taal- en letterkunde. pp. 658-661
Characterisation of a DNA tetrahedron and its interaction with sequence-specific ligands
No full textNanostructures engineered from DNA are becoming increasing popular in interdisciplinary research laboratories globally due to their ease to design and build, as well as their huge potential as devices for applications such as drug delivery and biosensors. Although many studies have used these structures alongside molecules that interact with DNA, little has been done to understand the effects of nanostructured DNA on ligand binding, and vice versa. In this thesis, a simple DNA tetrahedron, folded from four oligonucleotides, is used as a model in which to assess ligand interactions for a variety of DNA-binding agents.We characterised two DNA tetrahedron designs by band shifts, cleavage assays, chemical probing and melting studies. One of these tetrahedra, based on a previous design, was modified to incorporate single binding sites on each edge for a number of sequence-specific DNA-binding ligands: minor groove binders Hoechst 33258 and distamycin, intercalators actinomycin D, echinomycin, TANDEM and nogalamycin, and major groove binding triplex forming oligonucleotides. Band shifts, DNase I footprinting, global melting with UV and SYBR green I, as well as location-specific melting with fluorescent beacons were used to assess the interaction of these ligands with the DNA tetrahedron compared to linear duplex DNA.The results found actinomycin was able to inhibit folding of the structure, whereas other ligands did not. Sequence-selectivity was generally consistent across linear duplex and nanostructured DNA, though some changes in secondary binding sites were observed, however, Hoechst, distamycin and actinomycin showed reduced affinity to the nanostructure. Global thermal melting studies showed that all the ligands enhance stability, however, fluorescence beacons placed at specific locations found that many of the ligands were able to stabilise regions of the tetrahedron remote from their binding sites, including across junctions.The work described helps present a clearer understanding of ligand interactions with DNA nanostructures, as well demonstrates a model in which to assess structure stability at specific locations within a DNA nanostructure. This will aid the design and function of future DNA-based devices
Development and application of a QM/MM method for free energy calculations
No full textIn this thesis, a simplified Quantum Mechanics/Molecular Mechanics (QM/MM) method has been developed, which incorporates Density Functional Theory (DFT) and Free Energy Perturbation (FEP) to calculate QM/MM corrections for classically obtained MM free energies. This method has been applied to the calculation of hydration free energies and protein-ligand binding free energies. The hydration free energy study showed that for small organic compounds the QM/MM method could perform as well as standard MM. Further analysis highlighted that implementing QM/MM appeared to over-polarise for compounds with hydrogen bonding moieties. This over-polarisation was caused by the embedding technique utilised. Hence, the embedding strategy was adapted to utilize a Gaussian blurring approach, which enabled the minimisation impact of this over-polarisation. For the protein-ligand studies three proteins were investigated; COX-2, neuraminidase and CDK2. The results from these studies showed that the QM/MM method obtain less accurate results than conventional MM. This has been attributed to several factors, including; too simplistic embedding techniques leading to over polarisation for extremely polar protein-ligand systems (neuraminidase) and poor agreement for protein-ligand systems with small pocket sizes. Also, GCMC simulations have identified that erroneous system setup for CDK2 is the root cause of extremely poor correlation in both MM and QM/MM free energy studies
STRUCTURAL CHANGE AND INEQUALITY: SKILL PREMIA, FIRM SELECTION AND POLITICAL CONSEQUENCES
No full textCarter Michael G., Sībawayhi’s Principles. Arabic Grammar and Law in Early Islamic Thought. Atlanta, G., Lockwood Press, 2016
No full textSartori Manuel. Carter Michael G., Sībawayhi’s Principles. Arabic Grammar and Law in Early Islamic Thought. Atlanta, G., Lockwood Press, 2016. In: Bulletin critique des annales islamologiques, n°33, 2019. pp. 1-2
The major outer membrane protein gene of Chlamydia as a phylogenetic chronometer
No full textSIGLEAvailable from British Library Document Supply Centre- DSC:DX178141 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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