1,721,044 research outputs found

    Development of a new duplex real-time polymerase chain reaction assay for detection and quantification of KIHPyV and WUHPyV in pediatric samples

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    KI and WU are two recently-described human polyomaviruses worldwide distributed. An association between these two viruses and respiratory infections has been suggested, in particular in children and immunocompromised patients. Since no standardized detection methods are currently available, we developed a duplex real-time PCR assay for the simultaneous detection of KIHPyV and WUHPyV polyomaviruses based on TaqMan probes. We assessed this technique on 831 nasopharyngeal aspirate specimens from hospitalized paediatric patients with respiratory symptoms, retrospectively analysed with commercial multiplex assay for 16 other major respiratory viruses. Our assay detected 500 copies/mL for both KIHPyV and WUHPyV in all tested samples. We detected KIHPyV and WUHPyV genome in 28 (3.36%) and 41 samples (4.93%) respectively. Moreover, in 3 samples, the co-infection of the two viruses was found. 55 out of all positive samples (n=69) for KIHPyV and/or WUHPyV infection exhibited a co-infection with one or more respiratory viruses, confirming that KIHPyV and WUHPyV were often detected in association with other viral infections. Interestingly, KIHPyV and WUHPyV were detected singularly in 8 out of 28 cases and 6 out of 41 cases, respectively, suggesting a possible direct role of these viruses in the respiratory diseases. In conclusion, this method could be taken into account as an alternative technical approach to detect KIHPyV and/or WUHPyV in respiratory samples helping in the definition of the pathogenic potential and of the epidemiological prevalence of these two viruses

    Un selfie a 'un passo dal cielo'. La sostenibilità del turismo cinematografico e televisivo

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    Il contributo si propone come il primo studio di ambito italiano focalizzato sulla sostenibilità dei fenomeni turistici connessi ai prodotti audiovisivi. Vi si presenta una rassegna degli studi internazionali riguardanti il film-induced tourism incentrati che indagano gli impatti positivi e negativi sull’ambiente e sulle comunità. Si individuano, essenzialmente, un primo nucleo di riflessioni risalenti già alle ricerche iniziali sul film-induced tourism (a cavallo tra la fine degli anni Novanta e la metà degli anni Duemila) e un secondo nucleo più recente, rispetto al quale si prendono in esame in particolare gli apporti del gruppo di ricerca di Sangkyun Kim, aventi l’ambizione di categorizzare in modo più sistematico tali impatti. Si conduce quindi un’analisi originale su un caso di studio italiano individuato come emblematico: i fenomeni di overtourism al lago di Braies, in Alto Adige, connessi alla serie RAI Un passo dal cielo, menzionati nella letteratura italiana sul cineturismo unicamente in relazione alle “efficaci” politiche di marketing adottate dalla Regione altoatesina. Totalmente assenti erano invece i riferimenti agli effetti sull’ambiente, sull’esperienza turistica e, soprattutto, sulla vita quotidiana degli abitanti della Valle di Braies, effetti che hanno infine costretto la produzione a spostare le riprese in Veneto. Tutto ciò a fronte di ingenti investimenti pubblici (prima i finanziamenti erogati alla produzione della serie, poi i costi di elaborazione e implementazione delle misure che è stato necessario adottare per tamponare gli impatti dell’overtourism) e degli scarsi benefici economici registrati dagli esercizi locali, solo limitatamente interessati da flussi che erano prevalentemente di escursionisti in giornata (e che andavano anzi, potenzialmente, a discapito del turismo stanziale). Il contributo mette in luce tutti gli aspetti sopra illustrati attraverso un’analisi desk basata su molteplici fonti (articoli di giornale, siti e documenti istituzionali, report, sentenze di cause legali), con lo scopo di categorizzare gli impatti positivi e negativi dei flussi cineturistici a Braies, correlandovi anche le misure intraprese per limitare gli effetti negativi e per massimizzare quelli positivi. I risultati della ricerca confermano molte acquisizioni della letteratura internazionale, mettendo in luce, al contempo, alcune peculiarità del caso analizzato determinate, ad esempio, dalle caratteristiche morfologiche del territorio, dalla sua organizzazione in termini di viabilità e dal target turistico prevalente. In conclusione, lo studio si propone di ampliare la conoscenza e la valutazione critica degli impatti del film-induced tourism, a beneficio soprattutto dei soggetti istituzionali (in primis, le film commission) deputati ad assistere le produzioni audiovisive nell’individuazione delle location di ripresa. A fronte, infatti, di una diffusa consapevolezza in Italia in merito agli impatti ambientali delle riprese audiovisive, che ha portato all’attivazione di protocolli finalizzati al loro contenimento, manca del tutto nel contesto nazionale un’attenzione agli effetti anche negativi dei potenziali fenomeni turistici collegati, spesso protagonisti, al contrario, di retoriche entusiastiche che ne rilevano unicamente i benefici

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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