1,720,999 research outputs found
The Dual Role of Subaerial Biofilms through the Lens of AI: the case for Causal Networks and Targeted Learning
Subaerial biofilms (SABs) are microbial communities that form on surfaces exposed to both air and periodic moisture and that can adapt to harsh environmental conditions like UV radiation, and fluctuating temperatures. On the one hand they can protect built surfaces by forming a barrier against environmental stressors, on the other they can also cause deterioration through biological weathering. The balance is complex and depend on a large number of factors. Unfortunately, only a small part of the complex multiscale network of physical, chemical and biological processes is captured by existing mechanistic model; this prompts for the involvement of phenomenological models. In this work we point at the modeling advantages offered by Bayesian Networks (BNs), Causal Networks (or Structural Causal
Models, SCMs) and Targeted Learning (TL). The three related frameworks can model the complex interactions between biofilms, substrates, and atmosphere by identifying cause-effect relationships. SCMs can help devise interventions able to keep the biofilm within a desirable range of conditions, furthermore they can help assess the transportability of the discovered causal models across settings; Targeted Learning helps focusing on a specific research question (such as estimating the influence of factors on a target variable; quantifying their interactions; distinguishing between direct and mediated influence) without requiring the full discovery of the joint variable distribution, and using a semiparametric approach, as free as possible from modeling biases. In this work we highlight the considerable potential of the use of those related frameworks to the study of SABs
Methodological challenges for the investigation of the dual role of biofilms on outdoor heritage
Biofilm deterioration and biofilm protection should be considered as different aspects of the complex interactions between microbes and the surfaces of outdoor heritage (e.g. stones, bricks, mortar and plaster). Thus, it is urgent to verify and quantify to what extent the biofilm can protect from different weathering processes, to eventually determine the advisability of biofilm removal from the heritage surfaces. On one hand, it is necessary to more precisely describe the decaying processes caused by the microorganisms and to quantify the extent, severity, and rate at which the microorganisms are causing the decay. On the other hand, it is necessary to define methodologies to comprehensively study the bioprotection phenomena. So far, no decision-making tool is available to guide heritage professionals in deciding whether to remove or keep biofilms on heritage surfaces, and aesthetical alteration and discoloration is often the only criterion considered. In this work the different available approaches for the study of the dual role of biofilms on outdoor heritage have been critically reviewed. The open challenges and questions are also summarised
Biofilm colonization of metamorphic lithotypes of a renaissance cathedral exposed to urban atmosphere
Stone architectural heritage exposed outdoor represents a challenging habitat for biological growths; nevertheless, biocolonization on heritage structure is ubiquitous and represents a major mechanism of alteration. However, the identification of specific microorganisms with known reactivity towards the stone substrate does not necessarily imply that a biodeterioration process is in progress and, in specific conditions, bioprotection effects have been highlighted as a result of colonization. The main objective of the present research is to evaluate the biofilm formation on different lithotypes exposed to similar environmental polluted conditions, and to investigate whether the presence of subaerial biofilms can be associated to an increased magnitude of deterioration of the colonized surfaces with respect to the not colonized ones. In particular, the research examines the extensive biological colonization of the stone surfaces of the façade of the Cathedral of Monza (Italy). Four metamorphic stones widely used in the façade and showing rather different compositional, mineralogical and microstructural features were studied. The state of conservation of the stones was characterized under the mineralogical and compositional point of view by X-ray diffraction and Fourier Transformed infrared analysis. The microstructure of colonized substrates and of reference not colonized ones was studied by means of optical and electron microscopy, to comparatively evaluate the damage extent and weathering patterns in both conservative conditions. The structure and the architecture of biofilms growing on different lithic surfaces were investigated by CLSM in both fluorescence and reflection modes. Captured images were analyzed for 3D reconstructions of biofilm samples. The biovolumes were also calculated to estimate the total biomass. The results indicate that the four lithotypes showed different colonization extents. However, even in presence of extensive biological growth, chemical-physical deterioration mechanisms caused by environmental exposure were largely responsible for deterioration. A relationship between compositional and surface morphological features and biocolonization was also observed
Ecological strategies of bacterial communities in prehistoric stone wall paintings across weathering gradients. A case study from the Borana zone in southern Ethiopia
Rock art paintings represent fragile ecosystems supporting complex microbial communities tuned to the lithic substrate and climatic conditions. The composition and activity of these microbial communities associated with different weathering patterns affecting rock art sites remain unexplored. This study aimed to explore how bacterial communities adapt their ecological strategies based on substrate weathering, while also examining the role of their metabolic pathways in either biodeterioration or bioprotection of the underlying stone. SEM-EDS investigations coupled with 16S rRNA gene sequencing and PICRUSt2 analysis were applied on different weathered surfaces that affect southern Ethiopian rock paintings to investigate the relationships between the current stone microbiome and weathering patterns. The findings revealed that samples experiencing low and high weathering reached a climax stage characterized by stable microenvironments and limited resources. This condition favored K-strategist microorganisms, leading to reduced α-biodiversity and a community with a positive or neutral impact on the substrate. In contrast, moderately-weathered samples displayed diverse microhabitats, resulting in the prevalence of r-strategist bacteria, increased α-biodiversity, and the presence of specialist microorganisms. Moreover, the bacterial communities in moderately-weathered samples demonstrated the highest potential for carbon fixation, stress responses, and complete nitrogen and sulfur cycles. This bacterial community also showed the potential to negatively impact the underlying substrate. This research provided valuable insights into the little-understood ecology of bacterial communities inhabiting deteriorated surfaces, shedding light on the potential role of these microorganisms in the sustainable conservation of rock art
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Contact angle analysis of biocolonized stone surfaces: Comparative study of benchtop and portable approaches to advance on-site applications
While traditionally confined to laboratory settings, recent developments have enabled Water Contact Angle (WCA) measurements to be conducted on-site, under field conditions. This study presents a comparative evaluation of a conventional benchtop method and a portable instrument applied to uncolonized and biocolonized stone surfaces. A reference non-absorbing substrate was used for method validation. Results showed good agreement between the two methods on non-absorbing surfaces, confirming the reliability of the portable approach. On porous biocolonized substrates, the methods differed in absolute WCA values but showed consistent trends, while there was good agreement among drop absorption times. These findings demonstrated that the portable approach can effectively capture water-related properties on-site, with minimal invasiveness and high reproducibility. The study introduces a validated and statistically supported methodology for on-site wettability and water absorption assessment and emphasizes the influence of biofilm composition on surface water-related properties, contributing to the broader understanding of SAB-induced surface modification
Dispelling the Myths Behind First-author Citation Counts
We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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