1,720,957 research outputs found
In vitro propagation of three species of aquatic mosses (Bryophyta), anatomic description and biotechnological potential.
No full textThe use of mosses in biotechnology is an ever-expanding area of interest. Traditionally, mosses have
often been used as medicinal plants and nowadays can be a great source of secondary metabolites
with pharmacological activity (Mishra et al., 2014). In agriculture formulations based on bryophyte
extracts have been developed and used as natural pesticides, biopesticides or for their allelopathic
effects (Nozaki et al., 2007). Another important aspect is their application as bioindicators for their
ability to accumulate polluting substances, such as heavy metals, modifying, as response, their
secondary metabolism.
We showed as aquatic mosses can be used in phytoremediation/phytofiltration of polluted waters as
a biofilter having the ability to absorb heavy metals such as Pb, Cd, and Cr in a short time (Papadia
et al., 2020) and their ability to capture inorganic nanoparticles revealing a potential tool capable of
purifying water from nanostructured materials reducing the toxicity associated with the ingestion of
contaminated drinking water (De Matteis et al., 2021). To further expand research in the field of
biotechnology, solid in vitro propagation culture protocols are needed and an extensive knowledge of
in vitro adapted lines.
Laboratory protocols for axenic in vitro culture of moss were adapted to three species of aquatic moss:
Leptodictyum riparium (Amblystegiaceae), Vesicularia montagnei and Taxiphyllum barbieri
(Hypnaceae). The anatomic characters for their description and physiologic monitoring were
described and their biotechnological potential started to be explored. Treatments with
phytohormones, cytokinin (BAP) and auxin (NAA), showed a different response in the three species.
Selected the best growing condition, stereoscope was used to analyze different qualitative characters,
and light microscope was used to observe and measure the laminal and alar cells of the leaf with
differences between the three species after that cycle induction conditions were tested to study the
various life stages. Not all species adapt completely growing in vitro and may be suitable for different
purposes. The metabolic profile was also analyzed with 1H-NMR and analysis of metals and trace
elements were analysed using ICP/AES to highlight the differences between the three species
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dittrichia viscosa selection strategy based on Nip1.1 Gene for Arsenic phytoremediation
No full textArsenic uptake by plants occurs primarily through the root but it is not well known. Dittrichia viscosa
uptake and translocation of As is not fully understood and some data are contradictory. In one study
is reported that As is fully translocated to D. viscosa shoots and volatilized, while in another is show
that As is stabilized in the roots. In a previous work we studied the accumulation of As[III] and As[V],
confirming the high variability among individuals. We established several experimental clonal
populations to perform a functional study on the aquaporin NIP1.1.
D. viscosa is not a hyperaccumulator plant but can grow in high drought conditions while still
producing large biomass, even tolerating significant concentrations of As[III], As[V] and Cd[II]. In
spite of these remarkable characteristics, adaptive modification of performances is not predictable in
wild populations.
Genetic stability is a desirable trait and interest in the genetic improvement of this plant species is
increasing. We test a strategy that take in consideration the use of a clonal population of D. viscosa
with a defined NIP1.1 expression level and the in-vitro micropropagation technique to quickly scaleup the number of plants to perform specific HMs phytoremediation of contaminated areas
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Micropropagation of Viola ucriana Erben & Raimondo a critically endangered taxon for the Mediterranean region
No full textPlant tissue culture is a technique in which plant cells, tissues or organs are isolated from mother plants and
growth on artificial media. It is a powerful tool that could be used not only for micropropagation of crops,
decorative plants, and production of metabolites but also for biodiversity conservation purposes. In-vitro
conservation, based on micropropagation, is considered as one of the most efficient methods for ex-situ
conservation. Moreover, it could be considered strategic for preventing biodiversity losses and for reinforcing
populations in regions of the word known to be rich in biodiversity like Mediterranean area. In particular, this
region is rich of endemic plants species that evolve differently to adapt better to a specific environment. For
this reason, it has been recognized as one of the first 25 Global Biodiversity Hotspots. Unfortunately, the
biodiversity of this region is threatened by human activity. One example is represented by Viola ucriana. Ucria’s
violet is a rare plant which has been recognized as Critically Endangered Species according to International
Union Conservation for Nature (IUCN) Red List Criteria. In addition, it is included in the top 50 of the
threatened plant species of Mediterranean islands. Ucria’s violet appear to be near to the extinction and
preservation measures are needed. Shoot tips were obtained from germinated seeds collected near the peak of
Mt. Pizzuta (Palermo, Sicily) and successfully sub-cultured in-vitro. We developed an efficient
micropropagation protocol for Ucria’s in which we combined the use of two different plant growth regulators
(BAP and Zeatin) at different concentration. This protocol increased the laboratory plants population but a
specific concentration of Auxin was needed to produce plants with a complete developed root apparatus. The
protocol permitted us to produce well developed plant useful for reintroduction and translocation activity.
The first trial will take place in Autumn in the University of Salento botanical garden
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
- …
