311 research outputs found

    Molecular cloning and function analysis of FAD2 gene in Idesia polycarpa

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    Fan, Ruishen, Li, Long, Cai, Gui, Ye, Jing, Liu, Minhao, Wang, Shuhui, Li, Zhouqi (2019): Molecular cloning and function analysis of FAD2 gene in Idesia polycarpa. Phytochemistry 168: 1-10, DOI: 10.1016/j.phytochem.2019.112114, URL: http://dx.doi.org/10.1016/j.phytochem.2019.11211

    Identification of Quantitative Trait Locus for Overwintering Germinability in Rice (Oryza sativa L.)

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    312 recombinant inbred lines (RILs) in F9 from a cross between a overwintering cold-tolerant germplasm resource Glutinous rice 89-1(Gr 89-1) and a cold-sensitive variety Shuhui 527 was used for quantitative trait locus (QTL) analysis. The scores of percent ratooning germinability (PRG) and overwintering germinability (POG) were evaluated. The overwintering germination rate of axillary buds was scored to represent the overwintering germinability. Two significant QTLs (qPRG-4 and qPRG-7) on chromosomes 4 and 7 were detected and explained 8.3 and 7.2% of the total phenotypic variation, respectively. Three significant QTLs (qPOG-2, qPOG-3 and qPOG-7) were identified and mapped on chromosomes 2, 3, and 7, respectively. These QTLs contributed 9.6, 6.7, and 17.8% of phenotypic variations, respectively. A comparative analysis using SSR markers closely linked to the three QTLs for the overwintering revealed cold-tolerant individuals, which harbour the Glutinous rice 89-1 alleles at RM7110, RM250, RM418, and RM232, had a high percent overwintering germinability, while cold-sensitive individuals, which carry Shuhui 527 alleles at these loci, had a low percent overwintering germinability in the F2 population of Shuhui 527/Glutinous rice 89-1. This study demonstrated the utility of these SSR markers for selection of overwintering germinability genotypes

    High-Resolution Paleomagnetic Secular Variation Since ∼13 ka From a Loess Section in Northwest China and a Regional Geomagnetic Directional Model for East Asia

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    Original data of this paper have been uploaded to the MagIC database (https://earthref.org/MagIC/19798) (Fan et al., 2024a). The MATLAB executable file of SCHA.EAS14k model which can generate the paleosecular variation curves of the geomagnetic field directional elements within a spherical cap of 25° centered at 30ºN, 108ºE has been uploaded to the EarthRef database https://earthref.org/ERDA/2729 (Fan et al., 2024b).Paleomagnetic secular variation (PSV) records provide important information for the dynamic processes of the Earth's geomagnetic field, and also can be used for regional stratigraphic correlation. We conducted a paleomagnetic study on a high sedimentation rate Holocene loess section (the Minle section) with precise 14C age constraints in Northwest China. Rock magnetic results indicate that single domain and pseudo-single-domain magnetites are the main magnetic carriers of the natural remanent magnetization. Combining the alternating field demagnetization and thermal demagnetization results, we obtained a high-resolution PSV record with both absolute declination and inclination since ∼13 ka. Combining with previously published sedimentary records in East Asia, we developed an East Asian PSV reference curve over the past 14 ka (EASed14k) by relocating all the data to a common location (30ºN, 108ºE) and averaging them through Fisher statistics. We further generated a regional geomagnetic directional model spanning the past 14 ka for East Asia (SCHA.EAS14k), using the in situ PSV records and applying the revised spherical cap harmonic analysis in space and cubic B-spline in time. The predicted directional curves from this model at the common location are consistent with the EASed14k curves, which represent the first-order variation of the geomagnetic field in this area. The established reference curve and regional model for East Asia in this study will be used for regional and global comparison of the geomagnetic field.We express our gratitude to the editor team (Mark J. Dekkers and Agnes Kontny) and two anonymous reviewers for their constructive comments. We thank Min Zhang, Yifei Hou, Kaixian Qi, Yukai Luo and Hongyi Qiu for their helpful discussions. This work was supported by the National Natural Science Foundation of China (42488201, 42388101, 41974077, 41621004). S.C. acknowledges support from the Key Research Program of the Institute of Geology and Geophysics (CAS Grant IGGCAS-201905) and the Chinese Academy of Sciences (CAS) Pioneer Hundred Talents Program. J.X. acknowledges support from the Second Tibetan Plateau Scientific Expedition and Research Program (STEP) (Grant 2019QZKK0704).Peer reviewe

    A magnetic measurement technique for small rare samples: pre-study for Chang'e-5 returned and other extraterrestrial samples

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    Standard-size samples are usually used for traditional paleomagnetic study. In some cases, small irregular samples are employed considering their amount and magnetic strength as well as experiment efficiency. With the development of deep space exploration, the demand for studying small and magnetic weak samples is increasing. In this study, we established a magnetic measurement technique for small rare samples. We designed low-background, sample-nondestructive and high-flexibility holders with various materials for different measurements of small samples. With the customized holders, we estimated uncertainty and reliability of magnetic measurements of small specimens from a terrestrial basalt. The results indicate that susceptibility and remanence of small specimens (e.g., 2-mm cubes) are more affected by measurement position than larger specimens (³ 5 mm), which reminds us to pay special attention to consistency of the small specimen position during series of measurements. Moreover, susceptibility and remanence of small sister specimens (2-mm and 5-mm cubes) are more scattered than those of large ones (cube length ³ 1 cm), mainly due to inhomogeneity of magnetic mineral abundance in the sample. Nevertheless, the average magnetic results of different-size specimens are generally consistent within error, indicating the inhomogeneity of small specimens can be averaged out and demonstrating the reliability of small samples during magnetic measurements. Finally, we tested our measurement system with a lunar meteorite, which mimics the Chang’e-5 returned samples in both size and mineralogy. The main minerals of the meteorite are plagioclase, pyroxene, troilite and kamacite. The IRM normalized paleointensity method recovered the expected laboratory field while the ARM corrected paleointensities are about 2-3 times the expected field, where the latter can be explained by inapplicability of the empirical f¢ to the meteorite specimens. The ARM and IRM paleointensity calibration factor f¢ and a of the lunar meteorite were calculated to be 3.89 and 3138 μT, respectively, which provides new constraints for the empirical values. The magnetic results of the lunar meteorite demonstrate the efficiency of our measurement system for small weak samples. This study provides technical support for paleomagnetic study of the Chang'e-5 and other extraterrestrial samples in the future

    Development of a 3D active movement extent discrimination apparatus for testing proprioception at the ankle joint with inversion movements made in plantarflexion

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    Proprioception plays an important role in joint stability, and ankle sprains usually happen involving plantarflexion, internal rotation, and inversion. However, ankle 3D movement proprioception has never been measured in weight-bearing. Accordingly, the active 3-dimensional movement extent discrimination apparatus (AMEDA-3D) was developed and its reliability and validity were investigated. A total of 58 subjects volunteered for this trial, with 12 subjects with chronic ankle instability (CAI) and 12 healthy controls in the test–retest reliability study. There were 17 subjects with CAI and 17 healthy controls in the validity study. An intraclass correlation coefficient (ICC) and Minimum Detectable Change at the 90% confidence interval (MDC90) were computed. AMEDA-3D scores were analysed by independent samples t-tests, and Youden's index was used to calculate the optimal AMEDA-3D cut-off for discriminating individuals with CAI. Pearson's correlation analysis was used to explore the relationship between AMEDA-3D scores and Y Balance Test (YBT), Time In Balance Test (TIB), and Cumberland Ankle Instability Tool (CAIT) scores. The main results were as follows: (1) The ICC(3,1) value of AMEDA-3D scores was 0.817 (95% CI = 0.452–0.945) in CAI subjects. (2) The AMEDA-3D proprioceptive area under the curve score used to discriminate CAI subjects from healthy controls was 0.778, with a sensitivity of 94% and a specificity of 82%. (3) AMEDA-3D proprioceptive scores were moderately correlated with CAIT scores (r = 0.58 and p &lt; 0.001), YBT (r = 0.47 and p = 0.005), and TIB (r = 0.68 and p &lt; 0.001). Our findings suggest that the AMEDA-3D tool shows good reliability and validity for clinical assessment of proprioceptive deficits associated with CAI. Improved ankle 3D motor proprioception may positively impact subjects' balance control, self-rated symptoms, and function.</p

    黑龙江省小南山遗址受热历史的考古磁学证据

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    Archaeomagnetic techniques provide important qualitative and quantitative analysis methods for heating history of archaeological sites. The Xiaonanshan site, located at northeastern Heilongjiang Province, is one of the most important prehistoric sites in northeast China. The earliest discovered human activity relics on this site trace back to the Late Pleistocene. The site unearthed series of remains and relics of human activity since 16 ka, including many stone artifacts, some potteries, and a few remains of suspected fire use related to human lifestyles. In this study, we collected soil samples from the suspected fire areas and surround area, and conducted a series of rock magnetic experiments, including low-frequency magnetic susceptibility (χLF), step-wise variation of susceptibility versus temperature (χ-T), hysteresis loop, isothermal remanent magnetization (IRM) acquisition curves, back field demagnetization curves (BFD) and first order reversal curve (FORC) diagrams, to provide quantitative data for the heating history, e.g., heating temperature and heating range, of the site. The results indicate that the two suspected fire areas (ZB, ZC) show higher χLF, higher saturation IRM, stronger signal of FORC, and better thermal stability between 400~600℃, while the suspected fire area ZA, with surface soil removed during excavation, and surrounding area show lower χLF, lower saturation IRM, weaker signal of FORC, and gradually thermal alteration during the heating process indicated by χ-T curves. The rock magnetic results support the two suspected fire areas ZB and ZC were burnt, while ZA and surrounding areas were probably not heated, at least not to high temperatures as ZB and ZC. The thermal stability between 400~600℃ and obviously thermal transformation at 700℃ of the χ-T curves indicate that the historical heating temperatures of firing areas are about 600~700℃.No Full Tex
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