170,084 research outputs found
Scoprire il paesaggio del quotidiano. Un progetto di educazione al patrimonio culturale nella periferia urbana
No full textL’educazione al patrimonio culturale e al paesaggio è nodale per formare i giovani a una cittadinanza attiva e responsabile, alla tutela dei beni comuni, a stili di vita sostenibili e all’apprezzamento della diversità culturale e del dialogo intergenerazionale. Non può dunque limitarsi a episodi sporadici e a beni e contesti di eccellenza, ma deve essere transdisciplinare, inclusiva e praticabile in ogni luogo.
Questo libro racconta il progetto di ricerca e azione “Scuola Attiva Risorse (ScAR)”, vincitore del Polisocial Award che premia la ricerca a fini sociali del Politecnico di Milano. Il testo descrive un’azione sperimentale e innovativa condotta all’interno di un contesto fragile come quello delle periferie urbane, un processo partecipativo che ha coinvolto scuole, università, istituzioni culturali, amministrazioni e attori privati nell’interpretazione e nella valorizzazione dei patrimoni culturali “nascosti” nei quartieri di margine di Milano
DISCOVERING THE EVERYDAY LANDSCAPE A cultural heritage education project in the urban periphery
No full textHeritage and landscape education is crucial to training
young people in active and responsible citizenship,
protection of the public assets, appreciation of the
cultural diversity and intergenerational dialogue.
Therefore, it cannot be limited to sporadic experiences
and on outstanding heritage and contexts but must be
transdisciplinary, inclusive and practicable everywhere.
This book relates the research and action project
“Scuola Attiva Risorse” (ScAR), winner of the Polisocial
Award that recognizes research for social purposes
at the Politecnico di Milano. The text describes an
experimental and innovative action delivered within
the fragile context of the urban peripheries. This
participatory process involved schools, universities,
cultural institutions, administrations and private actors
in interpreting and enhancing the “hidden” cultural
heritage in Milan’s fringe neighbourhoods
MOESM14 of Comparative Subsequence Sets Analysis (CoSSA) is a robust approach to identify haplotype specific SNPs; mapping and pedigree analysis of a potato wart disease resistance gene Sen3
No full textAdditional file 14. Assessment of the depth cut-offs on the CoSSA results. Assessment of the effect of the lower and upper depth cut-off on the CoSSA output. (A) CoSSA output without the lower depth cut-off (R-bulk specific k-mers with a depth from 2 to 22 ×), (B) CoSSA output with the lower and upper cut-offs (R-bulk specific k-mers with a depth from 10 to 22 ×), (C) CoSSA output without the upper depth cut-off (R-bulk specific k-mers with a depth from 10 to ∞). Red: k-mers inherited from Kuba (resistance specific k-mers), blue: k-mers inherited from Ludmilla, green: k-mers inherited from both parents, grey: k-mers inherited from none of the parents. (D) Signal to noise ratio (SNR) for Sen3 and Ludmilla_chr9. Black: SNR for CoSSA results when no lower cut-off is applied (2 to 22 ×), stripped: SNR for CoSSA results when an upper and a lower cut-offs are applied (10 to 22 ×), grey: SNR for CoSSA results when no upper cut-off is applied (22 to ∞)
Morphometric Analysis of Dysplastic Nodules in Hepatitis C Virus–Related Liver Cirrhosis: Comparison with Cirrhotic and Large Regenerative Nodules
No full textObjective: To apply a computerized morphometric model to evaluate and quantify the morphologic features, including hepatic progenitor cells, in large regenerative nodules (LRN) and high grade dysplastic nodules (DN) in hepatitis C virus (HCV)–related cirrhosis.
Study Design: Thirty-two cirrhotic nodules; 10 LRN; and 8 DN were identified in cirrhotic livers with HCV-related cirrhosis removed at transplantation. All specimens were stained for routine diagnosis with hematoxylin-eosin and immunohistochemically for CD31, CD34, cytokeratin 7 (CK7) and reticulin. We determined by a computerized morphometric model on hematoxylin-eosin slices the volume fractions occupied by hepatocyte nuclei and cytoplasm, sinusoids, portal triads, fibrosis and centrilobular veins. We also investigated volume fraction of hepatocytes expressing CK7, and volume fractions of capillary units and of sinusoid capillarization expressing CD31 and CD34, respectively, and surface fraction of reticulin.
Results: Compared to LRN, DN showed higher volume fraction of hepatocyte nuclei, higher number of hepatocytes in unit volume, higher nuclear/cytoplasmic ratio, higher volume fractions of capillarized sinusoids, capillary units and CK7 positive hepatocytes and lower mean hepatocyte volume and surface reticulin fraction.
Conclusion: Our morphometric model is an objective method of quantification of the morphologic changes of LRN and DN, including the hepatic progenitor compartment. (Anal Quant Cytol Histol 2010;32:106–113
TGF-β1 activates RSC96 Schwann cells migration and invasion through MMP-2 and MMP-9 activities
No full textFollowing peripheral nerve injury, remnant Schwann cells adopt a migratory phenotype and remodel the extracellular matrix allowing axonal regrowth. Although much evidence has demonstrated that TGF-β1 promotes glioma cell motility and induces the expression of extracellular matrix proteins, the effects of TGF-β1 on Schwann cell migration has not yet been studied. We therefore investigated the cellular effects and the signal transduction pathways evoked by TGF-β1 in rattus norvegicus neuronal Schwann RSC96 cell. TGF-β1 significantly increased migration and invasion of Schwann cells assessed by the wound-healing assay and by cell invasion assay. TGF-β1-enhanced migration/invasion was blocked by inhibition of MMP-2 and MMP-9. Consistently, by real-time and western blot analyses, we demonstrated that TGF-β1 increased MMP-2 and MMP-9 mRNA and protein levels. TGF-β1 also increased MMPs activities in cell growth medium, as shown by gelatin zymography. The selective TGF-β Type I receptor inhibitor SB431542 completely abrogated any effects by TGF-β1. Indeed, TGF-β1 Type I receptor activation provoked the cytosol-to-nucleus translocation of SMAD2 and SMAD3. SMAD2 knockdown by siRNA blocked MMP-2 induction and cell migration/invasion due to TGF-β1. TGF-β1 also provoked phosphorylation of MAPKs extracellular regulated kinase 1/2 and JNK1/2. Both MAPKs were upstream to p65/NF-kB inasmuch as both MAPKs’ inhibitors PD98059 and SP600125 or their down-regulation by siRNA significantly blocked the TGF-β1-induced nuclear translocation of p65/NF-kB. In addition, p65/NF-κB siRNA knockdown inhibited the effects of TGF-β1 on both MMP-9 and cell migration/invasion. We conclude that TGF-β1 controls RSC96 Schwann cell migration and invasion through MMP-2 and MMP-9 activities. MMP-2 is controlled by SMAD2 whilst MMP-9 is controlled via an ERK1/2-JNK1/2-NF-κB dependent pathway. (Figure presented.)
Landscape Stories. Racconti visuali sul paesaggio del quotidiano/Landscape Stories. Visual Storytelling on the Everyday Landscape
No full textNarration is central in the knowledge building process about the landscape and its cultural value. The
enhancement of the everyday landscape, in particular, requires a shared interpretative process based
on intercultural and intergenerational dialogue. The essay refers to a field research experience involving primary and secondary school students in a process of discovery, interpretation and communication of the material and immaterial cultural heritage enclosed in the landscape of the urban suburbs.
The paper presents different forms of narrative used during the project, in particular visual storytelling
and new media narratives (traditional and digital route maps, mental maps, logbooks, videos, digital
storytelling, serious games). Through the project, young students experienced multiple ways to describe the landscape and the cultural heritage. In this way children and teenagers have approached
the disciplinary languages of architectural and landscape representation and discovered the different
heuristic and communicative potentialities of different methods and tools (analog and digital
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
La Forma Maris del Salento ed il Progetto WebGIS subacqueo
No full textIl progetto ha come scopo la realizzazione di un’applicazione in rete per la gestione dati cartografici e alfanumerici relativi alle evidenze archeologiche subacquee e costiere del Salento. Il reale obiettivo di questo programma però non è tanto l’applicazione in sé quanto il raggiungimento della massima condivisione possibile dei dati (fino ad arrivare ad una sorta di “Facebook geografico”), cercando di superare le tradizionali resistenze in questo senso; ci si propone quindi lo scopo di garantire la massima visibilità al prodotto e alle sue applicazioni, al lavoro degli archeologi, al contributo degli appassionati, ai problemi di tutela, alla fruibilità del patrimonio sommerso
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