1,721,221 research outputs found
Special Issue: Molecular Properties and the Applications of Peptide Nucleic Acids
No full textPolyamide analogs of DNA, or peptide nucleic acid (PNA), were first proposed in 1991 by a group of chemists and biochemists in a memorable Science paper [1].[…
Effect of chirality in gamma-PNA: PNA interaction, another piece in the picture
No full textModification of the PNA backbone can be used to broaden their utility by introducing new functional groups. In particular, gamma-modified PNA have been found to be quite effective in a number of applications, and exhibit particularly high DNA binding affinity. The introduction of one side chain imply that the achiral backbone of PNA becomes chiral, and binding properties depend on the stereochemistry. A new paper on gamma-modified PNA by Ly and co-workers complete the existing knowledge by displaying that in binding to complementary PNA stereochemical orthogonality can be demonstrated. This opens the way to the exploitation of stereochemical features in diagnostic assays and in nanofabrication
CORRADINI Roberto - Method for selective inhibition of human n-myc gene in n-myc expressing tumors through antisense and antigen peptido-nucleic acids (pna) - N. US7947657 del 24/05/2011
No full textThe present invention refers to sense and antisense peptide nucleic acids (PNAs). The present invention furhter refers to the use of said PNAs for trating genetic disease
Application of new specific probes in ion-exchange hplc analysis of DNA for the detection of GMO in foods
No full textProperties of L-phenylalanine tetraamides as chiral selectors for D,L-amino acids by capillary gas chromatography
No full textA wide series of L-phenylalanine tetraamide selectors (Phe-n-O-TA) were synthesized and used as stationary phases for chiral resoln. of D,L-amino acids by capillary gas chromatog. The influence of length and polarity of the oxaalkanoyl bridge spacing the diamide chains of the selectors and the effects of steric hindrance on sepg. power were studied. The characteristics of the chiral columns and the sepn. factors of D,L-n-butyl-N-trifluoroacetyl amino esters are reported
Detection of Non-Amplified Genomic DNA
No full textThis book offers a state-of-the-art overview on non amplified DNA detection methods
and provides chemists, biochemists, biotechnologists and material scientists with an
introduction to these methods. In fact all these fields have dedicated resources to the
problem of nucleic acid detection, each contributing with their own specific methods and
concepts. This book will explain the basic principles of the different non amplified DNA
detection methods available, highlighting their respective advantages and limitations.
The importance of non-amplified DNA sequencing technologies will be also discussed.
Non-amplified DNA detection can be achieved by adopting different techniques. Such
techniques have allowed the commercialization of innovative platforms for DNA detection
that are expected to break into the DNA diagnostics market. The enhanced sensitivity
required for the detection of non amplified genomic DNA has prompted new strategies
that can achieve ultrasensitivity by combining specific materials with specific detection
tools. Advanced materials play multiple roles in ultrasensitive detection. Optical and
electrochemical detection tools are among the most widely investigated to analyze non
amplified nucleic acids. Biosensors based on piezoelectric crystal have been also used to
detect unamplified genomic DNA. The main scientific topics related to DNA diagnostics
are discussed by an outstanding set of authors with proven experience in the field
Photonic Crystal Fibers for Physical, Chemical and Biological Sensing
No full textRemarkable developments and improvement have been observed in the field of optical fiberbased sensors in the last decade, for physical, chemical and biological applications. Many new sensors for specific analytes have been reported, novel sensing chemistries or transduction principles have been introduced, and applications in various fields have been realized. This boost has been pushed also by the mature fabrication technology of photonic crystal fibers whose peculiar properties well fit requirement for efficient sensing systems. Discussions on the main issues related to the use of photonic crystal fibers in biosensing applications is reported. Specific description of fiber functionalization for the detection of biologically relevant analytes is described highlighting deposition procedures of selective probes
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