47,772 research outputs found
Post-test analysis of the LOBI-Mod2 small break test A1-82 by Relap5/mod2 Code
No full textThe application of the current version of the Relap5 code to the experiment B-302 performed in LOBI test facility is discussed in this report. LOBI was a Pressurized Water Reactor (PWR), simulator of German 4-loop KWU-Siemens reactor, installed in the EC center of Ispra Italy supported by EURATOM. A1-82 is a Small Break Loss of Coolant Accident (SBLOCA) experiment part of the A-matrix of tests originally available only to German scientists.. The activity targets are:
a) Further code validation – assessment
b) Expanding the database of code application also in view of adopting the accuracy for UMAE and (later-on) CIAU uncertainty method application
c) Training of scientists.
The validation activity is performed by adopting the latest available techniques and procedures developed at UNIPI including nodalization qualification, use of acceptability thresholds and qualitative and quantitative evaluation of accuracy. Furthermore the activity (A-Test analysis) took benefit from the release by the German government of all details of the design of the LOBI facilit
Alendronate rescued osteoporotic phenotype in a model of glucocorticoid-induced osteoporosis in adult zebrafish scale
No full textLong-term effects of glucocorticoid treatment in humans induce bone loss and increase the risk of fracture in the skeleton. The pathogenic mechanisms of glucocorticoid-induced osteoporosis (GIOP) are still unclear. The GIOP and its effects have been reproduced in several animal models including Danio rerio (zebrafish) embryo. The treatment of adult fish with prednisolone (PN) has shown a dose-dependent decrease of mineralized matrix in the scales. Large resorption lacunae are characterized by single TRAP-positive cells which migrate to the margin of the scale merging into a multinucleated structures. The treatment with PN of cultured scales did not increase TRAP activity suggesting that the massive presence of osteoclasts in the resorption sites could be likely the result of a systemic recruitment of monocyte-macrophage precursors. We observed that treatment with PN induced a significant decrease of the alkaline phosphatase (ALP) activity in scale scleroblasts if compared with untreated controls. Then, we investigated the total mineral balance under prednisolone treatment using a time-dependent double live staining. The untreated fish fully repaired the resorption lacuna induced by prednisolone, whereas treated fish failed. The presence of osteoclast resorption fingerprints on new matrix suggested that the osteoclast activity counterbalances the osteodepositive activity exerted by scleroblasts. The treatment with PN in association with alendronate (AL) has surprisingly resulted in a significant decrease of TRAP activity and increase of ALP compared to PN-treated fish in biochemical and histological assays confirming the action of alendronate against GIOP in fish as well in human
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