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ALFANI, F (ALFANI, F); CANTARELLA, L (CANTARELLA, L); CIRIELLI, G (CIRIELLI, G); GALLIFUOCO, A (GALLIFUOCO, A); CANTARELLA, M (CANTARELLA, M)
No full textSucrose bioconversion in membrane reactors
No full textThe use of soluble and immobilized biocatalyst membrane reactors has been investigated in connection with the hydrolysis of sucrose. Both β-fructofuranosidase (EC 3.2.1.26) and Saccharomyces cerevisiae cells were tested either as free catalysts or differently immobilized in polyalbumin gels and in hydrogels of poly-2-hydroxyethylmethacrylate (both particles and films). Invertase activity and thermal stability up to 60°C were measured. Activity recovery of immobilized cells is much higher than that of immobilized enzymes and ranges between 36% and 89%, compared to free systems and depends on the immobilization procedures used. Thermal stability of yeast cells is higher than that of enzymes. Experiments carried out with sucrose concentrations between 40 mM and 1 M indicate the possible presence of substrate inhibition for free cells and, for immobilized cells, an invariance of the reaction rate towards sucrose concentration for values greater than 300 mM
Kinetics and stability of enzymatically active hydrogels in water-organic solvent systems
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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