1,720,972 research outputs found
Laparoscopic dissection of the ureter in deep-infiltrating endometriosis (DIE) of the pelvis using ultrasonically activated shears
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Detection and genotyping of human papillomavirus in cervical samples from italian patients.
No full textHuman papillomaviruses (HPVs) are etiological
agents of cervical cancer. In order to assess the
epidemiological incidence and frequency of different
HPV types, we applied a polymerase chain
reaction (PCR)-direct sequencing approach based
on the use of MY09/MY11 primers as compared
to Hybrid Capture assay. Cervical samples were
taken from 1,500 women, both with normal and
abnormal cytological smears, and we found an
incidence of 6.6% of HPV infection in Brescia.
Overall, 97 samples tested HPV-positive, yielding
18 HPV types. The four most frequent HPV types
were: HPV 16, -31, -6, and -58. This approach
could be used in ordinary laboratory settings for
quick and reliable typing of known and novel
HPVs from clinical specimens and it could also
be applied to anti-cancer vaccine development
Laparoscopic management of a large urethral leiomyoma
Full text linkIntroduction and hypothesis: A 42-year-old female presented with a 12-cm mass bulging the anterior vaginal wall and causing urgency urinary incontinence and bulk symptoms. Methods: Imaging showed a tumor originating from the dorsal and cranial part of the urethra and developing in the vesicouterine space and vesicovaginal septum, dislocating the bladder ventrally and the uterus cranial-dorsally. Results: Tranvaginal biopsy showed a benign leiomyoma. A laparoscopic approach with development of the vesicouterine space permitted a safe partial morcellation of the myoma. After the bladder and vaginal wall had been completely freed, further caudal dissection was conducted with isolation of the distal cranio-dorsal portion of the urethra. The dissection plane with the vaginal wall was developed up to the caudal margin of the urethral myoma almost corresponding to the vulvar plane, and total excision of the lesion was performed. Conclusion: Laparoscopic management of urethral leiomyomas that develop into the vesicouterine space and vesicovaginal septum is feasible and safe also for very large lesions
A new laparoscopic-transvaginal technique for rectosigmoid resection in patients with endometriosis.
No full textPrevalence and distribution of single and multiple HPV infections in cytologically abnormal cervical samples from Italian women.
No full textThe prevalence of single and multiple HPV infections was assessed over a cohort of 213 women with cytological abnormalities and its association
with cervical neoplasia established. Roche linear arrayHPVgenotyping testwas used to identifyHPVgenotypes. The most prevalentHPVgenotypes
in cervical cancer samples were HPV16 (61.2%), HPV52 (16.1%), HPV18 (12.9%) and HPV 31 (9.6%). Multiple HR and LR HPV infections,
comprising between two and 5+ HPV types, were identified in 49.7% of samples, with a significantly lower number in severe dysplasia and cervical
cancer samples (p < 0.05).
These results seem to indicate that detection of multiple HPV infection with HR-HPV types is not significantly better as a predictor of cervical
cancer than single HR-HPV infection, though further longitudinal studies are needed to better clarify the relevance of these infections to the
progression of cervical neoplasia
Parametrial endometriosis with ureteral involvement: A case report of a conservative approach without ureteral resection
Full text linkIntroduction: Parametrial endometriosis could often involve the ureter and periureteral tissue causing hydronephrosis and distortion of the normal ureteral course and position. The treatment in the case of hydronephrosis could be ureterolysis (with or without the positioning of a stent) or a ureteral resection. Materials and methods: This is the case of left parametrial endometriosis with hydronephrosis in a young woman. The preoperative work-up showed a deep infiltrating endometriosis of the left parametria, the left uterosacral ligament, and the left ureter which caused hydronephrosis. The patient was symptomatic (dysmenorrhea, dysuria, and pelvic pain). In this video, we focused on ureterolysis: all the endometriotic tissues were removed, the ureter was completely released, and the decision was to place a stent in the ureter without ureteral resection. Results: The post-operative course was normal, the stent was changed after 1 week (from mono-J to double-J) and removed 1 month after surgery. Follow-up at 3 and 8 months after stent removal was normal, and renal function was normal. The last follow-up, after 13 months, showed no hydronephrosis, no signs of deep infiltrating endometriosis, and normal renal function. The patient continues with oral contraception. Conclusion: Ureterolysis could be an effective alternative to ureteral resection in cases of deep infiltrating endometriosis even when the ureter is involved with hydronephrosis
Comparison of the AMPLICOR Human Papillomavirus Test and the Hybrid Capture 2 Assay for detection of high-risk human papillomavirus in women with abnormal PAP smear
No full textInfection with human papillomavirus (HPV) is a necessary step in the progression to cervical cancer. Many methods for HPV testing are
currently available, mostly developed to detect pools of HPV types. Hybrid Capture 2 (HC2) is one of the most widely used. A new PCR-based
assay, the Roche AMPLICOR HPV test, has been recently developed. Both assays recognize a group of 13 HR HPV types contemporaneously.
This study evaluated the performance of both methods for detecting high-grade cervical lesions as a part of management for abnormal PAP
smears. The study population was composed of 213 women, all referred to colposcopy and histologic diagnosis following an abnormal PAP test.
Biopsy-confirmed high-grade cervical intraepithelial neoplasia was used as a gold standard. Overall agreement was 84.9% with a kappa value of
0.6. When comparing the ability to detect moderate cervical intraepithelial neoplasia (CIN2+) and high-grade cervical intraepithelial neoplasia
(CIN3+/cancer), AMPLICOR proved slightly more sensitive than HC2, a finding that is important when HPV testing is used in a triage of borderline
smear results. Genotyping of discordant results showed a prevalence of LR-HPV types in HC2 positive/AMPLICOR negative samples, and a similar
prevalence of HR- and LR-HPV types in AMPLICOR positive/HC2 negative samples. In conclusion, the study shows that the AMPLICOR assay
is more sensitive than HC2, which makes it a valid alternative for routine clinical use
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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