1,720,962 research outputs found

    Influence of postharvest water stress on lipoxygenase and alcohol dehydrogenase activities, and on the composition of some volatile compounds of Gewürtztraminer grapes dehydrated under controlled and uncontrolled thermohygrometric conditions

    No full text
    Gewürztraminer grapes with a sugar content of around 212 g/L (21.7oBrix) were dried at 17oC, 40% relative humidity and 1.5 m/sec air flow in a 300 L thermo-conditioned tunnel. Control grapes were dried traditionally in a window ventilated room, under uncontrolled environmental conditions varying with outside climate. Tunnel-dried grapes reached the desired sugar concentration (305 g/L, 29.5oBrix) in 17 days, loosing 36% of their weight. Control grapes lost only 22% of their weight and grey mould developed in several bunches at the last sampling. Titratable acidity decreased for tunnel-dried and control grapes from 6.5 g/L to 4 g/L and 5 g/L, respectively. Lipoxygenase (LOX) activity declined in both samples from 120 to 90 U/mg protein dw, with a subsequent significant increase after 20% weight loss in tunnel-treated grapes while the control grapes showed a small peak (150 U/mg protein dw) at 13% weight loss. Six carbon compound evolution showed a loose correlation with LOX activity. Alcohol dehydrogenase specific activity and the concentrations of ethanol and of acetaldehyde plus ethyl acetate showed fluctuating patterns of change, with the evolution of these three variables showing similarity, particularly evident in the tunnel-dried grapes. Carotenoids declined significantly, to increase slightly at the end of the experiment in both samples, with the decline more rapid in the control grapes. Traditional, uncontrolled conditions, did not permit constant dehydration, and provoked a rapid stress to the berries (10% of weight loss). Controlled conditions permitted uniform dehydration, postponed water stress, giving a higher quality product without loss of berries

    Short-Term Postharvest Carbon Dioxide Treatments Induce Selective Molecular and Metabolic Changes in Grape Berries

    No full text
    Detached wine grapes (Vitis vinifera cv. 'Trebbiano', white skinned) were treated for 3 days with 30 kPa of CO2 and then transferred to air for an additional 9 days to partially dehydrate (about 20% weight loss). At the end of the CO2 treatment on withering berries, total polyphenols and flavonoids were maintained in the skin, but to a more limited extent in the pulp. An induction of the proanthocyanidin synthesis appeared to be one of the responses to the treatment because both (+)-catechin and (−)-epicatechin concentrations increased in the skin. The skin and pulp of the grape berries showed different molecular responses to a high CO2 treatment. As revealed by microarray hybridizations, 217 and 75 genes appeared differentially expressed in the skin and pulp of treated samples, respectively. Functional categorization and gene enrichment analyses pointed out that epicarp cells undergo more pronounced changes in transcript profiling at the end of the incubation period. Highly represented categories in both tissues were related to protein, stress, transcript, RNA, and hormone (ethylene, ABA) metabolism. Fermentation, CHO metabolism, and redox regulation functional categories were represented only in the skin

    Going Beyond Counting First Authors in Author Co-citation Analysis

    Full text link
    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Effects of Postharvest Partial Dehydration and Prolonged Treatments with Ethylene on Transcript Profiling in Skins of Wine Grape Berries.

    Full text link
    or certain food products, postharvest controlled stresses or treatments with specific elicitors are applied to induce desired physical/chemical changes and/or to positively affect phytochemical content. This is the case of wine grapes where both strategies, singularly applied or coupled, can be used to modulate berry composition and, as a consequence, affect wine quality traits. Since the knowledge of the effects of these postharvest treatments on berry metabolism and the regulation of gene expression is very limited, a large-scale transcriptome analysis has been carried out, using an oligo-based microarray (14,562 probes) on skins of wine grape (Vitis vinifera L.) berries subjected to dehydration, at different rates, up to 30% of weight loss or to ethylene treatment (500 ppm for 7 days) after harvest. A number of differentially expressed targets was detected following both treatments, indicating that grape berries are still reactive at advanced stages of postharvest dehydration and that ethylene induces marked changes in transcriptome after harvest also in non-climacteric fruit such as grape berries

    Variations on the Author

    Full text link
    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

    Full text link
    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
    corecore