42 research outputs found
Replication Data for: Charting the lipopeptidome of non-pathogenic Pseudomonas
No full textA major source of pseudomonad specialized metabolites are the non-ribosomal peptide synthetases (NRPSs) assembling siderophores and lipopeptides. Cyclic lipopeptides (CLPs) of the Mycin and Peptin families are frequently associated with - but not restricted to - phytopathogenic species. We conducted an in silico analysis of the NRPSs encoded by lipopeptide biosynthetic gene clusters in non-pathogenic Pseudomonas genomes, covering thirteen chemically diversified families. This global assessment of lipopeptide production capacity revealed it to be confined to the Pseudomonas fluorescens lineage, with most strains synthesizing a single type of CLP. Whereas certain lipopeptide families are specific for a taxonomic subgroup, others are found in distant groups. NRPS activation domain-guided peptide predictions enabled reliable family assignments, including identification of novel members. Focusing on the two most abundant lipopeptide families (Viscosin and Amphisin), a portion of their uncharted diversity was mapped, including characterization of two novel Amphisin-family members (nepenthesin, oakridgin). Using NMR fingerprint matching, known Viscosin-family lipopeptides were identified in fifteen (type) species spread across different taxonomic groups. A bifurcate genomic organization predominates among Viscosin-family producers and typifies Xantholysin-, Entolysin- and Poaeamide-family producers but most families feature a single NRPS gene cluster embedded between cognate regulator and transporter genes. The strong correlation observed between NRPS system phylogeny and rpoD-based taxonomic affiliation indicates that much of the structural diversity is linked to speciation, providing few indications of horizontal gene transfer. The grouping of most NRPS systems in four superfamilies based on activation domain homology suggests extensive module dynamics driven by domain deletions, duplications, and exchanges
Estudio de sustancias antimicrobianas de interés biotecnológico y biomédico
Full text link"El incremento de bacterias multidrogo-resistentes (MDR) representa un problema de salud pública a nivel mundial. Actualmente, las terapias antimicrobianas disponibles no son suficientes para eliminar a patógenos MDR, por lo que resulta indispensable el estudio de antimicrobianos con nuevos mecanismos de acción que favorezcan el control de estos patógenos.
El uso de péptidos antimicrobianos (PAMs) ha sido sugerido como alternativa en el tratamiento de enfermedades infecciosas pues poseen mecanismos de acción diferentes a los de los antibióticos de amplio espectro; desafortunadamente, son pocos los PAMs que han sido aprobados para su uso terapéutico. Los PAMs del veneno de alacrán son un ejemplo de sustancias bioactivas con potencial para su uso contra bacterias MDR.
Isolation, partial purification and antimicrobial activity of a bacteriocin-like compound produced by Pseudomonas spp. EMM-1
No full textPéptidos antimicrobianos de alacrán
Full text linkEl veneno de los alacranes posee una gran variedad de componentes, entre los que se encuentran diversos tipos de toxinas que modulan la función de canales iónicos y receptores en membranas de células excitables, siendo las responsables de los múltiples síntomas conocidos de envenenamiento. Además de las toxinas, también se han aislado del veneno de alacrán péptidos antimicrobianos bioactivos contra bacterias, hongos, levaduras y virus. Sin embargo, se han evaluado pocos péptidos antimicrobianos de alacrán contra cepas bacterianas multiresistentes. Las infecciones bacterianas son un problema de salud pública y tienen una de las morbilidades más alta en el mundo. Parte de la explicación, se entiende por la multirresistencia adquirida por las bacterias y, por otra parte, por el lento desarrollo de nuevos antibióticos. Los péptidos antimicrobianos presentes en los venenos de alacrán, se posicionan como potenciales elementos terapéuticos para ser caracterizados y usados en el diseño y desarrollo de fármacos antimicrobianos de nueva generación.Programa Cátedras del Consejo Nacional de Ciencia y Tecnología, CONACYT (ID de Investigador: 2220)
Beca de Doctorado otorgado por CONACYT (No. de Becario: 538125/294272).
Vicerrectoría de Investigación y Estudios de posgrados de la Benemérita Universidad Autónoma de Puebla (Proyectos 2017; número de proyecto: 279)
Social rights and economics : claims to health care and education in developing countries
Full text linkThe author analyzes contemporary rights-based and economic approaches to health care and education in developing countries. He assesses the foundations and uses of social rights in development, outlines an economic approach to improving health and education services, and then highlights the differences, similarities, and the hard questions that the economic critique poses for rights. The author argues that the policy consequences of rights overlap considerably with a modern economic approach. Both the rights-based and the economic approaches are skeptical that electoral politics and de facto market rules provide sufficient accountability for the effective and equitable provision of health and education services, and that further intrasectoral reforms in governance, particularly those that strengthen the hand of service recipients, are needed. There remain differences between the two approaches. Whether procedures for service delivery are ends in themselves, the degree of disaggregation at which outcomes should be assessed, the consequences of long-term deprivation, metrics used for making tradeoffs, and the behavioral distortions that result from subsidies are all areas where the approaches diverge. Even here, however, the differences are not irreconcilable, and advocates of the approaches need not regard each other as antagonists.Health Monitoring&Evaluation,Health Systems Development&Reform,Decentralization,Public Health Promotion,Early Child and Children's Health,Health Monitoring&Evaluation,Health Economics&Finance,Poverty Assessment,Agricultural Knowledge&Information Systems,Gender and Education
Variar 'le prime 7 stanze della luna': ritrovati versi di ballo per Jacopo Peri
No full textBetween 1611 and 1620, Jacopo Peri (1561-1633) composed dances often, especially in the carnival entertainments, organized in "high room" (“Sala alta”) of the Palazzo Pitti in Florence. Then, the correspondence between Peri and the Gonzaga court, with whom he had ancient relations, keeps the news of a dance which he wrote to the duchess Catherine de 'Medici Gonzaga, which was to be represented at Mantua in the spring of 1620. It was thought that the libretto for this event was lost, in fact it exists and here for the first time gives you argumentative news of the discovery, as well as the full edition. Unrecognized, perhaps because of a little eloquent title ("Versi cantati nel ballo fatto dalla Sereniss. Sig. Duchessa di Mantova ...”), the printed libretto was lying in the University Library of Bologna. His identification was made possible by the review, through documents known and unknown, the festivities held in Mantua, in court and elsewhere, between Sunday, April 26 and Monday, May 4, 1620, a week in which the forceful preparations and celebrations for the birthdays of the Duke (April 26) and Duchess (May 2), and for the wedding of Maria Zati with Traiano Bobba (May 3-4). The music, as often happens, is untraceable, but the discovery of the verses is important because it adds a new source to the series, not very rich, of texts for dance, and it permits to enter the 'laboratory' of the author and the mechanisms of production era, including failed projects and implemented. Finally, the contextualisation of found libretto, allows us to clarify the involvement, sometimes misunderstood, by Claudio Monteverdi in those same festivities, and contributes to the debate on the dissemination of models of fiorentine and mantuan entertainment in the european courts.[...
Emerging Applications of Bacteriocins as Antimicrobials, Anticancer Drugs, and Modulators of The Gastrointestinal Microbiota
Full text linkThe importance of antimicrobial compounds produced by beneficial bacteria on the biocontrol of phytopathogens
No full textBacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and soil pollution, particularly in fields with damaged soils where pesticides and fertilizers have been indiscriminately used. Beneficial bacteria may increase plant health by inhibiting pathogenic microorganisms; some examples include Gluconacetobacter diazotrophicus, Azospirullum brasilense, Pseudomonas fluorescens, Pseudomonas protegens, and Burkholderia tropica. However, most studies showing the antagonistic potential of these bacteria have been performed in vitro, and just a few of them have been evaluated in association with plants. Several inhibitory substances involved in pathogen antagonism have not been elucidated yet; in fact, we know only 1 % of the bacterial diversity in a natural environment leading us to assume that many other inhibitory substances remain unexplored. In this review, we will describe the characteristics of some antimicrobial compounds produced by beneficial bacteria, the principal methodologies performed to evaluate their production, modes of action, and their importance for biotechnological purposes
Charting the lipopeptidome of nonpathogenic Pseudomonas
No full textPseudomonas species are prominent producers of lipopeptides that support proliferation in a multitude of environments and foster varied lifestyles. By genome mining of biosynthetic gene clusters (BGCs) with lipopeptide-specific organization, we mapped the global Pseudomonas lipopeptidome and linked its staggering diversity to taxonomy of the producers, belonging to different groups within the major Pseudomonas fluorescens lineage
Importancia de compuestos antimicrobianos producidos por bacterias benéficas en el biocontrol de fitopatógenos
No full textBacteria produce antimicrobial compounds to compete for nutrients and space in a particular habitat. Antagonistic interactions can be evaluated by several methodologies including the double-layer agar and simultaneous inhibition assays. Among the well-known inhibitory substances produced by bacteria are the broad-spectrum antibiotics, organic acids, siderophores, antifungal, and bacteriocins. The most studied bacterial genera able to produce these inhibitory substances are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia. Some beneficial bacteria can promote plant growth and degrade toxic compounds in the environment representing an attractive solution to diverse issues in agriculture and soil pollution, particularly in fields with damaged soils where pesticides and fertilizers have been indiscriminately used. Beneficial bacteria may increase plant health by inhibiting pathogenic microorganisms; some examples include Gluconacetobacter diazotrophicus, Azospirullum brasilense, Pseudomonas fluorescens, Pseudomonas protegens, and Burkholderia tropica. However, most studies showing the antagonistic potential of these bacteria have been performed in vitro, and just a few of them have been evaluated in association with plants. Several inhibitory substances involved in pathogen antagonism have not been elucidated yet; in fact, we know only 1 % of the bacterial diversity in a natural environment leading us to assume that many other inhibitory substances remain unexplored. In this review, we will describe the characteristics of some antimicrobial compounds produced by beneficial bacteria, the principal methodologies performed to evaluate their production, modes of action, and their importance for biotechnological purposes. Las bacterias producen compuestos antimicrobianos para competir por nutrientes y espacio en un hábitat particular. Las interacciones antagónicas pueden evaluarse mediante varias metodologías, incluido el agar de doble capa y los ensayos de inhibición simultánea. Las sustancias inhibidoras mejor conocidas producidas por bacterias incluyen antibióticos, ácidos orgánicos, sideróforos, antifúngicos y bacteriocinas. Entre los géneros bacterianos más estudiados que producen sustancias inhibidoras se incluyen Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia y Burkholderia. Algunas bacterias beneficiosas tienen la capacidad de promover el crecimiento de las plantas y degradar compuestos tóxicos en el ambiente, por lo que podrían incrementar el rendimiento de los cultivos y disminuir problemas de contaminación del suelo, especialmente donde los pesticidas y fertilizantes han sido utilizados indiscriminadamente. Algunas bacterias beneficiosas pueden aumentar la salud de las plantas al inhibir microorganismos patógenos, por ejemplo, Gluconacetobacter diazotrophicus, Azospirullum brasilense, Pseudomonas fluorescens, Pseudomonas protegens y Burkholderia tropica. Sin embargo, la mayoría de los estudios que muestran el potencial antagónico de estas bacterias se han realizado in vitro, y pocos de ellos se han evaluado en asociación con plantas. Varias sustancias inhibitorias implicadas en el antagonismo de los patógenos aún son desconocidas; de hecho, sabemos que solo se ha aislado el 1 % de la diversidad bacteriana en un ambiente natural, lo que sugiere que hay muchas otras sustancias inhibitorias que no han sido exploradas. En esta revisión describimos las características de algunos compuestos antimicrobianos producidos por bacterias beneficiosas, las principales metodologías usadas para evaluar su producción, modos de acción y su importancia para fines biotecnológicos
