1,721,062 research outputs found
Human B-cell clones recognize an immunodominant epitope on the HCV non structural region
No full textIdentification of an immunodominant B-cell epitope on the hepatitis C virus non structural region defined by human monoclonal antibodies.
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Production of human monoclonal antibodies to hepatitis C virus and their characterization
No full text: Human monoclonal antibodies (hMAb) provide novel ways to probe the B-cell repertoire in health and disease. However, the development of hMAb technology has met with several difficulties owing to the instability of the cell lines, the low level of specific antibody secretion, and the poor cloning efficiency, particularly when using lymphoblastoid cells (1,2). In order to overcome these problems, some investigators have fused human B lymphocytes with human/mouse myeloma heterohybrids. However, in such systems, human chromosomes are unstable and may occasionally be deleted. Despite the potential emergence of technical pitfalls, B-cell immortalization with EBV has been extensively used for hMAb production, because of its simplicity and because EBV can bind to and penetrate in virtually all B lymphocytes, theoretically allowing the exploration of the whole B-cell repertoire. The most recent protocols have made use of techniques aimed at expanding the population of antigen-specific B-cell precursors and improving the capacity of B-cells to grow at low density. These methods will be discussed below
Identification of an immunodominant B-cell epitope on the NS4 subregion of HCV defined by human monoclonal antibodies.
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Aspects de la reproduction chez Acanthocardia aculeata (L.) (Mollusca, Bivalvia) du Golfe de Trieste.
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Antibody-negative chronic hepatitis C virus infection in immunocompetent children
No full textAntibody-negative hepatitis C virus (HCV) infection, defined by the presence of HCV viremia in the absence of a serological response to HCV, was detected in two immunocompetent and symptom-free children; each had a history of exposure to blood products. HCV infection may occasionally explain cryptogenic elevation of aminotransferases, even in he absence of serum anti-HCV. HCV-RNA shoud be investigated in these cases, particularly in the presence of previous exposure to blood products
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