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[Decorated mortarboard belonging to Kaitlyn Ceh]
No full textPhotograph of a mortarboard cap decorated and worn by Kaitlyn Ceh in Spring 2025 commencement ceremony
CEH-60 is essential for normal mating contact and survival of oxidative stress.
No full text(A) The mating occupancy score of ceh-60 but not vrp-1 mutants is lower than wild-type animals, indicating a defect in mating contact that is not caused by lowered yolk protein production. Mating contact deficiency is rescued by expressing ceh-60 under the control of its own promoter (ceh-60p::ceh-60) or in the intestine (elt-2p::ceh-60) of ceh-60(lst466) mutant animals, but not when expressing PBC-truncated ceh-60 (ceh-60p::ceh-60(ΔPBC)). N = 3 for vrp-1(lst539). N ≥ 6 for other conditions. (B) Oxidative stress survival as measured by fraction of worms alive during incubation in 5 mM H2O2 is lower in ceh-60(lst466) mutants (■) than in controls (●). The down-regulation of VITs in vit-1 RNAi treated animals (○) or vrp-1(lst539) animals (□) does not cause increased susceptibility to oxidative stress. Expression of ceh-60 under its own promoter (ceh-60p::ceh-60) in ceh-60(lst466) mutants (▲) is able to rescue stress survival. (C) Oxidative stress survival in ceh-60(lst466) animals (■) is rescued by intestinal expression of ceh-60 (elt-2p::ceh-60, ▼) but not by expression of ceh-60 in the AWC neurons (odr-1p::ceh-60, ▽) or expression of ceh-60 with a truncated PBC-interaction domain (ceh-60p::ceh-60(ΔPBC), ◇). N ≥ 3. Error bars indicate SEM. ***p p S1 Data. RNAi, RNA interference; VIT, vitellogenin.</p
CEH-18 plays a role in host defence.
No full textA. Ratio of green fluorescence (GFP) to size (TOF) in wild-type (IG274) or ceh-18(mg57) mutant (IG1714) worms carrying frIs7, infected or not with D. coniospora for 16 h (yellow and blue, respectively; data for IG274 is as Fig 3B in [53]), and IG274 worms treated with RNAi against sta-1 (control) or ceh-18 and, from left to right, exposed to high salt (purple; cpsf-2(RNAi) is a positive control, sta-2(RNAi) a negative control [11]), in worms also expressing GPA-12* in the epidermis, and in the rde-1(ne219);wrt-2p::RDE-1 background and infected by D. coniospora. For the latter 2 panels, sta-2(RNAi) is a positive control. A minimum of 45 worms was used for each condition. The black bar represents the mean value; *** psta-1(RNAi), Dunn’s test; for the other conditions there is not a significance decrease. The results of the 3 right panels are unpublished results from [11], representative of at least 4 independent experiments. B. Quantitative RT-PCR analysis of the expression of several genes in the nlp-29 cluster in wild-type worms, sta-2 and ceh-18 mutants infected by D. coniospora; results are presented relative to those of uninfected worms. Data (with average and SD) are from three independent experiments. **, p C. Results of 2 independent tests of survival of rde-1(ne219);wrt-2p::RDE-1 worms treated with RNAi against sta-1, sta-2, akir-1 or ceh-18, infected with D. coniospora and cultured at 25°C (n>65 for all tests). The difference between the sta-1(RNAi) and ceh-18(RNAi) animals is highly significant in both trials (p<0.0001; one-sided log rank test).</p
CEH-28 and ZAG-1 Function in Regulating Differentiation of the C. elegans M4 Pharyngeal Neuron
No full textM4 is a multifunctional neuron in the C. elegans pharynx that can both stimulate peristaltic contractions of the muscles in the pharyngeal isthmus and function systemically to regulate an enhanced sensory response under hypoxic conditions. In this work, we extend our understanding of M4 function by investigating the roles of two transcription factors, CEH-28 and ZAG-1. We find that CEH-28 regulates a neuroendocrine signaling function of the M4 neuron by activating expression of the family gene dbl-1 gene, which encodes a TGF-β ligand, egl-17 gene, which encodes the FGF family ligand and the FMRF family gene, flp-5. DBL-1 secreted from M4 functions in an R-Smad independent TGF-β signaling pathway to affect g1 gland cell morphology. ZAG-1 functions in M4 and activates isthmus muscle contractions required for normal feeding. zag-1(hd16) null mutants completely lack isthmus peristalsis and are defective in serotonin signaling. Furthermore, ZAG-1 acts as an upstream regulator of ceh-28 and its downstream targets in M4, and regulates additional aspects of M4 differentiation by activating expression of M4 markers ser-7b and flp-2. Taken together, my work identifies a new hierarchical gene regulatory network in the M4 neuron, which regulates signaling and differentiation, and also provides insights regarding how multifunctionality of motor neurons is regulated
Countryside Survey: Publications by CEH/ITE staff
Full text linkThis document lists peer-reviewed papers and other
publications derived from Countryside Survey from 1978
to the present
Uterine blood flow evaluation in bitches suffering from cystic endometrial hyperplasia (CEH) and CEH-pyometra complex
Full text linkDoppler ultrasound is a useful diagnosis tool to evaluate uterine blood flow in different canine reproductive states. The aim of this study was to describe and compare uterine blood flow in bitches suffering from cystic endometrial hyperplasia (CEH) and CEH-pyometra complex. Ninety diestrous bitches were clinically, hematologically and ultrasonographically classified into 4 groups: 1) Clinical signs, leukocitocis, CEH and uterine luminal contents (CEH-P, n=31); 2) Asymptomatic bitches with CEH and uterine contents (CEH-C, n=15); 3) Asymptomatic bitches with CEH without uterine contents (CEH, n=16); and 4) Normal diestrus bitches (ND, n=28). The widest cross-sectional diameter (D) and uterine wall thickness (W) of uterine horns were measured using two-dimensional ultrasound. Peak systolic velocity (PSV) and end diastolic velocity (EDV) of uterine arteries were measured by Doppler ultrasound in all the bitches. Peak systolic velocity and EDV were higher in CEH-P than in CEH-C, CEH and ND (P 0.1). Conversely, RI showed lower values in CEH-P than in the other three groups (P 0.1). When all the bitches were considered, correlation between RI and D was r=-0.69 (P 0.1). It is concluded that the uterine artery blood flow velocity of bitches suffering from pyometra was higher, not only from normal bitches, but also from females with endometrial hyperplasia. Furthermore, endometrial hyperplasia, accompanied or not by luminal contents, had a higher blood flow velocity than normal uterus. Hemodynamic parameters appear as useful markers to differentiate uterine pathological conditions.Fil: Batista, Pablo Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Gobello, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; ArgentinaFil: Rube, A.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Docente; ArgentinaFil: Tórtora, M.. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Blanco, Paula Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Laboratorio de Nutrición Mineral y Fisiología Reproductiva; Argentin
<i>ceh-60</i> mutants have a more permeable cuticle.
No full text(A) Representative images of acridine orange staining in wild-type and ceh-60(lst466) animals. Dotted white lines in wild-type acridine orange images show worm outline. Scale bar = 200 μm. (B) Acridine orange stains ceh-60 mutants but not wild-type animals. Expressing wild-type ceh-60 under the control of its own promoter (ceh-60p::ceh-60) or under an intestinal promoter (elt-2p::ceh-60) rescues the defect in ceh-60(lst466) mutants, but neuronal (odr-1p::ceh-60), pharyngeal (myo-2p::ceh-60), or PBC-truncated (ceh-60p::ceh-60(ΔPBC)) expression does not. Fluorescence intensity is shown on a logarithmic scale for clarity. ****p N ≥ 20. Underlying data are available in S1 Data. A.U., arbitrary unit; NS, not significant.</p
Evidence for Pseudogap Phase in Cerium Superhydrides: CeH and CeH
Full text linkPolyhydride superconductors have been shown to possess metallic properties
with a Bardeen-Cooper-Schrieffer-type superconducting ground state. Here, we
provide evidence for unconventional transport associated with a pseudogap phase
in cubic cerium superhydride CeH ( = 116 K) at pressure
of 115-125 GPa. A large negative magnetoresistance in the non-superconducting
state below 90 K, quasi -linear electrical resistance, and a
sign-change of its temperature dependence mark the emergence of this phase. We
studied the magnetic phase diagrams and the upper critical fields
(T) of CeH, CeH, and CeD in pulsed fields up
to 70 T. (T) of CeH and CeD exhibits pronounced
saturation at low temperatures in accordance with the
Werthamer-Helfand-Hohenberg model, whereas CeH stands out in particular,
as it does not obey this model. Our observations, therefore, reveal the
unconventional nature of non-superconducting state of cerium superhydride
CeH.Comment: The discussion part has been improve
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