1,720,980 research outputs found
Segregation of heteroplasmic length variants in HVI region of mitochondrial DNA by size-based separation method.
No full textImmunolocalisation of desmoglein-1 in equine muzzle skin
No full textDesmogleins (Dsgs) are glycoproteins of the cadherin family that
allow the formation of desmosomes via a Ca2+-dependent
homophilic interaction. Desmosomes are cell-cell adhesion
systems that contribute to the mechanical integrity of epidermis by
linking to keratin intermediate filaments and, therefore, connecting
the cytoskeleton of adjacent keratinocytes (Cozzani et al. 1994).
On light microscopy, desmosomes appear as intercellular bridges.
On electron microscopy they consist of symmetrical disc-like
structures, which link the intermediate filaments system within
cells to the plasma membrane and to adjacent cells (North et al.
1999). Four isoforms of Dsgs have been described in human
(Whittock 2003), one in bovine (Koch et al. 1990; Puttagunta et al.
1994), 2 in canine (Muller et al. 2000; Aoki et al. 2002), 6 in
murine (Whittock 2003) and, recently, one in porcine (Nishifuji et
al. 2005) species. The production of autoantibodies against
desmoglein-1 (Dsg-1) leads to the loss of epidermal cell-cell
adhesion responsible for pustular lesions in the life-threatening
skin disease pemphigus foliaceus (PF) (McMillan and Shimizu
2001). The pathogenesis of PF has been intensively investigated in
man and the molecular characteristics of human Dsgs analysed
(Mahoney et al. 1999). In the canine species Dsg-1 has been
characterised and the hypothesis that this glycoprotein is the target
antigen in PF is strongly supported (Suter et al. 1993; Iwasaki et
al. 1997; Steeves et al. 2002). However, contrasting evidence
concerning a minor role of Dsg-1 in the pathogenesis of canine PF
has been reported (Olivry et al. 2006).
The first case of equine PF was reported by Barnick and
Gutzeit (1891) and since 1980 only a few such reports have been
documented in this species (Peter et al. 1981; Messer and Knight
1982; Rothwell et al. 1985; Laing et al. 1992; Wohlsein et al.
1994; Stahli et al. 2005). Recently, Vandenabeele et al. (2004) and
Zabel et al. (2005) reviewed equine PF.
In horses diagnosis is based mainly on clinical and
histopathological features, which strictly resemble those described
in other species and on the response to immunosuppressive drugs
(Vandenabeele et al. 2004; Zabel et al., 2005). Nevertheless, the
presence of Dsg-1 in equine epidermis has never been
documented and whether this transmembrane protein is targeted
by circulating antibodies in horses with PF is still unknown.
This study documents the presence of a pericellular antigen in
horse muzzle skin by immunohistochemistry using 2
commercially available monoclonal antibodies raised against
bovine Dsg-1-2 and human Dsg-1 and the presence of 2 bands by
immunoblotting analyses using anti-human-Dsg-1
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
PCR detection of Y specific amelogenin sequence in Turner syndrome by modified primer set.
No full text
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Analysis of aged seminal stains by current forensic DNA approach
No full text‘Cold’ cases involving sexual assault can be solved decades after investigations analysing DNA from stored evidences, collected when current forensic method were not developed. To evaluate the influence of time and storage conditions on presumptive test and DNA analysis, three aged seminal stains date back to the first half of the ‘900, recovered during restoration work of the Institute of Legal Medicine of the University of Bologna, were analysed by the semenogelin test, microscopic identification of spermatozoa, autosomal, Y-chromosome and X chromosome STRs, and mitochondrial DNA analysis. All samples displayed a weak positive reaction for semenogelin test and the sperm heads were clearly identified. The number of detectable autosomal and Y-STRs was strongly correlated to the DNA degradation index, except for one specimen showing a high degradation index but enough DNA to obtain a composite profile. STRs included in the Minifiler kit and smaller STRs in ESX System were reproducible in all analysed specimens. Aged seminal stains may be successfully analysed by current forensic methods, even if the selection of appropriate amplicons size based on DNA amount and its degradation index is mandatory to predict the success probability in casework
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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