1,721,035 research outputs found
Use of antibodies to probe the TNF alpha quaternary structure at bioactive levels: evidences for slow conversion of bioactive oligomers to inactive forms
No full textBinding of the glycopeptide antibiotic teicoplanin to D-alanyl-D-alanine-agarose: the effect of micellar aggregates.
No full textAffinity enhancement of complementary peptide recognition
No full textA peptide hydropathically complementary to Big Endothelin [Big ET] residues 16-29 has been synthesized in a multimeric form starting from an octadentate polylysine core, essentially in a way similar to the procedure used for the production of multiple antigenic peptides [MAP's]. Interaction between the multimeric complementary peptide [8-delta-ET] and the Big ET fragment 16-32 containing the target complementary region, also synthesized in a multimeric form [8ET], was evaluated by analytical high performance affinity chromatography and solid phase binding assays. While the binding interaction between the monomerics peptide pair was in the micromolar range, the recognition between the corresponding multimeric form was characterized by enhanced binding affinity of at least two orders of magnitude. In solution, complex formation between multimeric complementary peptide and target Big ET sequence in the monomeric and multimeric form was accompanied by precipitation at concentrations higher than 0.5 mg/mL and 0.1 mg/mL, respectively. Polyclonal antibodies raised against the multimeric target sequence recognized multimeric and monomeric ET target sequences with binding affinities similar to binding affinities exhibited by the multimeric complementary peptide. Multimerization of hydropathically complementary peptides could provide an improved opportunity to measure and thus probe quantitative binding properties of complementary peptides
Identification of an epitope of tumor necrosis factor (TNF)-receptor type 1 (p55) recognized by a TNF-alpha-antagonist monoclonal antibody.
No full textComparison of the solid phase enzyme receptor assay (SPERA) and the microbiological assay for teicoplanin.
No full textProduction of soluble tumor-necrosis-factor receptor-type-i in Escherichia-coli - optimization of the refolding yields by a microtiter dilution assay
No full textIn this study optimization of the soluble tumor necrosis factor receptor type I (sTNF-RI) refolding by the use of a micro-renaturation assay in 96-well microplates is described. Microplate wells were filled with buffers varying in pH and urea and substrate concentration. Denatured and reduced sTNF-RI was then rapidly diluted and allowed to refold for a variable time at different temperatures. The extent of renaturation was measured by a sandwich enzyme-linked immunosorbent assay (ELISA), based on the use of two monoclonal antibodies obtained against urinary sTNF-RI. Among about 100 different combinations tested, a maximum refolding yield of 21.5% has been obtained in 100 mM Tris, pH 8-8.5, 1 mM EDTA, 0.1% bovine serum albumin, 2 M urea, at a denatured protein concentration of 10 mu g/ml and at 26 degrees C. Folded sTNF-RI was purified by batchwise immunoaffinity chromatography and its activity evaluated by immunological and biological assays, A good correlation was observed between the data obtained with different assays (biological assay, ligand-directed ELISA, and double-determinant sandwich ELISA) indicating that the refolded receptor has gained biological and immunological reactivity comparable to those of the soluble TNF-receptor type I expressed in eukaryotic cells. (C) 1995 Academic Press, Inc
Il periodo romano, dalla formazione alla professione
No full textIl presente saggio si pone l’obiettivo di approfondire il lungo periodo, circa un quarto di
secolo, che Cirilli trascorre a Roma, tra Otto e Novecento, in primo luogo per svolgere il proprio
ciclo formativo, poi per porre le basi di una carriera lunga e prolifica, sempre percorsa sul
doppio binario della didattica e della professione, che ha come prima espressione compiuta il
villino-studio in viale della Regina
Determination, aerobic biodegradation and environmental occurrence of aliphatic alcohol polyethoxylates sulphates (AES)
No full textA new analytical method was developed for the routine specific determination of the anionic surfactant Alcohol polyEthoxylate Sulfate (AES) in environmental aqueous samples. An enrichment/fractionation of the target analytes in water samples was performed by solid-phase extraction (SPE) on graphitized carbon black (GCB) (recoveries: 90-103%), followed by hydrolysis/derivatization with fluorescent reagents and separation/detection by reversed-phase high performance liquid chromatography coupled with fluorescence (HPLC-FLD). The developed procedure was applied to the study of the aerobic biodegradation of AES under laboratory conditions and to a ten-month monitoring of AES, as well as of linear alkylbenzene sulfonates (LAS), nonylphenol polyethoxylates (NPE) and alcohol polyethoxylates (AE) surfactants, in the Po river (Northern Italy). The residual concentrations found in the river waters were compared and used for a preliminary estimation of the annual average loads of monitored surfactants in the Adriatic Sea
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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