1,720,977 research outputs found
Restriction endonuclease analysis of chromosomal DNA from Listeria monocytogenes strains
No full textRestriction endonuclease analysis of chromosomal DNA was applied to thirteen Listeria monocytogenes strains alongside the more conventional typing methods of serotyping and phage typing. The organisms were isolated from cases of sporadic listeriosis (nine strains); from an occasional nosocomial cluster (two strains); and from food samples (two strains). Purified DNAs were digested with EcoRI restriction endonuclease, and restriction fragments separated by electrophoresis. Restriction patterns correlated well with phage patterns, but also allowed typing of the phage-untypable strains. DNA fingerprinting appears to be a potentially helpful tool for epidemiological investigations of listeric infections, particularly when phage typing fails to determine the identity or diversity of the isolates
Interactions with lectins and agglutination profiles of clinical, food, and environmental isolates of Listeria.
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Emergence in Italy of a PPNG strain carrying "Asia" plasmid.
No full text.Emergence in Italy of a PPNG strain carrying "Asia" plasmid
Evaluation of a vero cell toxicity test to detect EHEC infection
No full textAn epidemiologic investigation was carried out in Modena (Italy) to evaluate the prevalence of faecal VEROtoxin (FVT) in diarrhoeal stool specimens. One thousand and sixty-six stool specimens, submitted to the Clinical Microbiology Laboratory of the University Hospital of Modena, were collected and faecal filtrates tested for neutralizable cytotoxin by a toxicity test on VERO cells. Cytopathic effect on VERO cells was produced by 301 stool specimens (28%); neutralizable VT was detected in 40 (13%) out of 301 positive samples (3.7% of 1066 specimens). The prevalent FVT type was VT2 (50%), followed by VT1 (32.5%) and VT1+2 (17.5%). We evaluated an assay that detects both VTs directly from stool specimens to demonstrate that enterohemorrhagic strains (EHEC) should be considerated a causative agent of sporadic non-bloody diarrhoea. Our results suggest that toxin neutralization assay is a sensitive and specific technique and may be used as an alternative method to diagnose diarrhoeal infections caused by EHEC
Su di un caso di Morbo di Hodgkin complicato da infestazione da Strongyloides Stercoralis
No full textViene riferito un caso di Morbo di Hodgkin complicato da grave infestazione da Strongyloides stercoralis. Lo Strongyloides è un parassita opportunista in grado di determinare quadri clinici particolarmente gravi, per autoinfestazione, in condizioni di immunodepressione. In questi pazienti la comparsa di sintomi gastrointestinali aspecifici deve indurre ad eseguire gli accertamenti necessari per evidenziare, accanto ai più comuni germi patogeni, anche la presenza di parassiti
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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