1,723,145 research outputs found

    Modelling of the citrus ccd4 family members: In silico analysis of membrane binding and substrate preference

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    Coloring is one of the most important characteristics in commercial flowers and fruits, generally due to the accumulation of carotenoid pigments. Enzymes of the CCD4 family in citrus intervene in the generation of β-citraurin, an apocarotenoid responsible for the reddish-orange color of mandarins. Citrus CCD4s enzymes could be capable of interacting with the thylakoid membrane inside chloroplasts. However, to date, this interaction has not been studied in detail. In this work, we present three new complete models of the CCD4 family members (CCD4a, CCD4b, and CCD4c), modeled with a lipid membrane. To identify the preference for substrates, typical carotenoids were inserted in the active site of the receptors and the protein–ligand interaction energy was evaluated. The results show a clear preference of CCD4s for xanthophylls over aliphatic carotenes. Our findings indicate the ability to penetrate the membrane and maintain a stable interaction through the N-terminal α-helical domain, spanning a contact surface of 2250 to 3250 Å2. The orientation and depth of penetration at the membrane surface suggest that CCD4s have the ability to extract carotenoids directly from the membrane through a tunnel consisting mainly of hydrophobic residues that extends up to the catalytic center of the enzyme

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Riqueza florística de las mesadas y su relación con la matriz circundante en los afloramientos basálticos centro-argentinos

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    Fil: Cantero, J. Universidad Nacional de Río Cuarto; Argentina.Fil: Cantero, J. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Mulko, J. Universidad Nacional de Río Cuarto; Argentina.Fil: Núñez, C. Universidad Nacional de Río Cuarto; Argentina.Fil: Amuchastegui, A. Universidad Nacional de Río Cuarto; Argentina.Fil: Barboza, G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Ariza Espinar, L. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Chiarin, F. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Sfragulla, J. Secretaría de Minería, Córdoba; Argentina.Fil: Sfragulla, J. Universidad Nacional de Córdoba; Argentina.Fil: Brandolin, P. Universidad Nacional de Río Cuarto; Argentina.Fil: Martínez, A. Universidad Nacional de San Luis; Argentina.Los afloramientos basálticos son considerados hábitats importantes para la conservación de biodiversidad. En la Sierra de Los Cóndores (Córdoba, Argentina), son frecuentes cerros basálticos con relieve cónico escarpado, en forma de mesada, rodeados por una matriz con materiales geológicos diferentes y más antropizada.Fil: Cantero, J. Universidad Nacional de Río Cuarto; Argentina.Fil: Cantero, J. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Mulko, J. Universidad Nacional de Río Cuarto; Argentina.Fil: Núñez, C. Universidad Nacional de Río Cuarto; Argentina.Fil: Amuchastegui, A. Universidad Nacional de Río Cuarto; Argentina.Fil: Barboza, G. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Ariza Espinar, L. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Chiarin, F. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Sfragulla, J. Secretaría de Minería, Córdoba; Argentina.Fil: Sfragulla, J. Universidad Nacional de Córdoba; Argentina.Fil: Brandolin, P. Universidad Nacional de Río Cuarto; Argentina.Fil: Martínez, A. Universidad Nacional de San Luis; Argentina.Ciencias de las Plantas, Botánic

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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    koamabayili/VECTRON-author-checklist: VECTRON author checklist

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    We have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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