1,721,146 research outputs found
The Many Faces of Autophagy: Balancing Survival and Cell Death
No full textAutophagy and apoptosis are two fundamental biological mechanisms that may cooperate or be antagonistic, and both are involved in deciding the fate of cells in physiological or pathological conditions [...
Flow cytometry and oncohematology: A relationship bound to last | [Citometria a flusso ed ematologia oncologica: Un matrimonio sempre valido]
No full textThe contribution of flow cytometry to the diagnosis and follow-up of hematological neoplasms is of paramount importance and cannot be overstressed. Today, this relationship is not questioned and it is unlikely that the new molecular techniques will be able to replace it shortly. The reason of this long lasting success relies on the importance of phenotype in the diagnostic work-out, but above all on the flexibility of the technique, which is able to produce clinically relevant information in different situations, using smart fluorescence-based method
EXPRESSION OF CYTOTOXIC EFFECTOR MOLECULES IN CD56BRIGHT NK CELLS GENERATED IN VITRO: IDENTIFICATION OF AN IMMATURE CD56BRIGHT/GRANZYME-B−/PERFORIN−/TRAIL+ NK CELLS.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
le colture cellulari in ambito biomedico-diagnostico
No full textFlow cytometry assays for viability and cell death. I. Necrosis, apoptosis and cell proliferation. Today, several
assays and antibodies are available for the analysis of apoptosis, cell cycle and DNA repair, cell viability and vitality,
proliferation, migration, adhesion, endo- and exocytosis. Many of the assays are fluorescence- or colorimetricbased,
offering sensitivity, practicability, as well as safety. These reagents have been validated on multiple instrument
platforms, including microscopy and flow cytometry analysers. Here we describe some advances in application of flow
cytometry particularly related to cell cycle analysis and cell proliferation studies, giving some general information and
reporting protocols currently used in laboratories. Particularly, fluorescent probes have been organized into categories
based on different aspects of apoptosis, necrosis and cell proliferation
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