50,983 research outputs found
Populations of Lobaria pulmonaria (L.) Hoffm. in the Cansiglio Regional Forest (Veneto, Pre-Alps, north-east Italy): Distribution, diversity and conservation issues
No full textThe distribution of the epiphytic macrolichen Lobaria pulmonaria (L.) Hoffm. was surveyed in the Regional Forest of Cansiglio, extending over 3,500 ha in the eastern part of the Veneto Pre-Alps (north-east Italy). Data on the main characteristics of the forest sites and on Lobaria trees were collected with the aim to evaluate the main ecological factors, related to forest composition and management, affecting the distribution of Lobaria pulmonaria . Lichen diversity was surveyed on 15 randomly selected Lobaria trees. A good correlation between macrolichens and the total number of species was found. The largest Lobaria populations are concentred in the north-eastern part of the forest, where mixed and multi-plane silver fir - beech formations prevail, and selective cutting is carried out. Our data has enabled us to develop some criteria for the identification of priority conservation sites for Lobaria pulmonaria and lichen diversity in the study area, improving the focus on biodiversity in the management of the forest. © 2006 Società Botanica Italiana
Genetic diversity of brown trout (Salmo trutta L., 1758) in central Italy.
No full textGenetic diversity was analysed in brown trout Salmo trutta populations living in an area of central Italy using RFLP analysis of two mtDNA segments and of the nuclear locus LDH-C1*. The data indicated a genetic structure profoundly altered by repeated stockings with allochthonous material of Atlantic origin. In fact, four and 11 of the haplotypes detected were, respectively, identical or genetically very close to haplotypes found in Danish populations, the putative source of stocked brown trout. Furthermore, the LDH-C1*90 allele, typical of north-western Europe, was widespread among the samples studied. Nonetheless, four populations are characterized by a high frequency of both putative autochthonous haplotypes and the LDH-C1*100 allele, common in the Mediterranean basin. These populations, sampled in areas where S. trutta is documented historically, might represent a remnant of the species' indigenous biodiversity, showing the scope for improving the management of brown trout in central Italy
Cord blood transplantation in children with haematological malignancies
No full textUmbilical cord blood transplantation (UCBT) is largely used to treat children affected by haematological malignant disorders. In comparison to bone marrow transplantation (BMT), advantages of UCBT include lower incidence and severity of graft-versus-host disease, easier procurement and prompter availability of cord blood cells, and the possibility of using donors having HLA disparities with the recipient. The large experience accumulated so far has shown that UCBT offers to children a probability of cure at least comparable to that of patients transplanted with bone marrow cells. Since it has been demonstrated that an inverse correlation between the number of nucleated cord blood cells infused per kg recipient body weight and the risk of dying for transplantation-related causes exists, recently developed strategies aimed at increasing the number of cord blood progenitors and at favouring stem cell homing could further optimize the outcome of children with leukemia or other malignancies receiving UCBT. © 2010 Elsevier Ltd. All rights reserved
c-myb proto-oncogene is expressed by quiescent fibroblasts and unlike B-myb gene does not correlate with proliferation
No full textPresenza di aplotipi “marmoratus” in popolazioni di trota fario (Salmo trutta L., 1758) dell’Italia centrale: transfaunazione o paleointrogressione?
No full textDirect involvement of the root associated microorganisms into essential oil metabolism in Vetiveria zizanioides (L.) Nash,
No full textVetiver root, essential oil, endophytic bacteria, Vetiver oil bioconversion, gas
chromatography/mass
The species Vetiveria zizanioides (L.) Nash is a perennial graminaceous plant native to north
India. Selected germlines of this plant species have long been cultivated for their odorous roots that
contain the essential oil of Vetiver which consists of a complex mixture of sesquiterpene
hydrocarbons and alcohols mostly used as a basic material for perfumery and cosmetics (Maffei
2002).
Electron microscope analysis of the root cells of Vetiveria zizanioides (L.) Nash revealed the
occurrence of bacteria as well as electrodense crystals of essential oils in the external layers of
cortical parenchymatous cells up to those close to the endodermis (Massardo et al. 2004). The close
relationship between bacteria and the essential oil stimulated the hypothesis of a direct involvement
of those endophytic bacteria in the essential oil metabolism.
The aim of the present study was to analyze the root-associated bacterial community of V.
zizanioides by using culture-based and culture independent approaches, and to investigate the
possible involvement of the root-associated microorganisms with the essential oil metabolism.
Planting in Campania Region of the species V. zizanioides native to Thailand and isolation of
endophytic bacteria were performed as previously described (Pontieri et al. 2005). Independent
bacterial isolates from the microbial community inside cell roots were molecularly controlled by
analyzing the differences in ribosomal 16S DNA. The bona fide endophytic bacterial species of V.
zizanioides roots included the cultivable Pseudomonas, Kluyvera, Enterobacter, Serratia and
Arthrobacter strain species. The ability of the bacterial strain isolates to grow using the essential oil
as a sole source was analyzed investigating the eventual modification in the oil molecular structure
caused by the bacterial growth in vitro. To this purpose, bacterial strain isolates were individually
cultivated in SRM-oil medium (NH4H2PO4 1g/l, KCl 0.2 g/l, MgSO4 0.2 g/l, replacing glucose with
a commercial DMSO-dissolved Vetiver oil). Successively, the Vetiver oil constituents (Massardo et
al. 2006) were extracted from the exhausted growth medium by means of Stirr Bar Sorptive
Extraction (Gerstel Twister) and subjected to cryodesoption followed by quantitative analysis by
Gas Chromatography-Mass Spectrometry (GC-MS). Here we show that bacteria living inside the
Vetiver roots are capable of oil biotransformation. These results open the intriguing and immediate
possibility to biotransform the molecular structure of the Vetiver oil either in vivo by acting on the
modulation of the plant root bacterial colonization/presence, or in vitro by means of bioconversion
processes
Iperplasia prostatica benigna (BPH); epidemiologia descrittiva e impatto economico sanitario
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