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Three women (L to R) A woman, Domingo Coronado and Mrs. Camero of New Mexico (photograph)
No full textThree women (L to R) A woman, Domingo Coronado and Mrs Camero of New Mexico
Crónica Romanística Hispalense
No full textEste artículo recoge los acontecimientos más importantes que han tenido lugar en el Área de Conocimiento de Derecho Romano de la Universidad de Sevilla du-rante el último año. Además de hacerse referencia a las actividades y seminarios organizados por el Área de Conocimiento, fundamentalmente se da cuenta de las publicaciones (monografías y manuales) que han visto la luz recientemente, así como de aquellas cuya publicación es inminente. Dentro del grupo de las mo-nografías, se comentan aquí las aportaciones más significativas de las siguientes obras: F. BETANCOURT-SERNA, La Recepción del Derecho Romano en Colombia (saec. XVIII). Fuentes codicológicas jurídicas 1: Ms. N° 274 BNC (en prensa); M. SERRANO-VICENTE, Custodiam praestare. La prestación de custodia en el Dere-cho Romano (Madrid 2006); y R. de CASTRO-CAMERO, Soluciones "in iure" a una controversia patrimonial: transacción, juramento y confesión (Sevilla 2006). Entre los Manuales publicados, se encuentran: F. BETANCOURT-SERNA, Derecho Romano Clásico. 3' ed. (Sevilla 2007); y R. de CASTRO-CAMERO, "Quid iuris?" Las razones del jurista en el Derecho Romano (Sevilla 2007).This article collects last year's main events in the Roman Law Area at the Uni-versity of Seville. Besides information about academic activities and seminars, it refers to the latest publications (monographs and manuals) in our field. The Monographs on Roman Law published by our research staff are these: E BE-TANCOURT-SERNA, La Recepción del Derecho Romano en Colombia (saec. XVIII). Fuentes codicológicas jurídicas I: Ms. N° 274 BNC (soon to be published); M. SERRANO-VICENTE, Custodiam praestare. La prestación de custodia en el De-recho Romano (Madrid 2006); y R. de CASTRO-CAMERO, Soluciones "in iure" a una controversia patrimonial: transacción, juramento y confesión (Sevilla 2006). Furthermore there are the following Roman law manuals for students: F. BETANCOURT-SERNA, Derecho Romano Clásico. 3' ed. (Sevilla 2007); y R. de CASTRO-CAMERO, "Quid iuris?" Las razones del jurista en el Derecho Romano (Sevilla 2007)
Investigating fibroblast growth factor and its role in canine osteosarcoma
No full textCanine osteosarcoma (OS) is a malignant neoplasia of the osteoblast, most often identified in the appendicular skeleton, which is both locally aggressive and highly metastatic. The standard of care treatment of amputation and adjuvant chemotherapy yields a median survival time of 10-12 months, an improvement in which has not been noted despite active research. The biologic behavior of cancers seems to be correlated to the state of differentiation of the tumor cell population. Differentiation is taken into account when a histopathologic grade is assigned to a particular tumor; poorly differentiated tumors acquire a higher grade and are expected to behave more aggressively. The tumor microenvironment contains many cells and signaling molecules such as cytokines, chemokines and growth factors. Investigating these factors may identify a stimulus for a less differentiated and more aggressive neoplasia. This is even more compelling if the stimulus is a druggable target.
Fibroblast growth factors (FGFs) interact with fibroblast growth factor receptors (FGFRs) to initiate cell signaling that is important in embryonic development, wound healing, and angiogenesis. FGF2, also termed basic FGF (bFGF), plays an important role in osteoblast proliferation and maintaining osteoblasts in an undifferentiated state.
We hypothesize that 1) canine OS cells will express FGFRs and FGF2, 2) FGF signaling blockade will attenuate pro-tumorigenic properties in OS cells, 3) FGF signaling blockade will cause enhanced differentiation of the OS cell lines, and 4) circulating FGF2 will be increased in canine OS patients compared to healthy controls and increased in dogs with osteoblastic OS compared to those with osteolytic OS.
We investigated FGFR gene expression with reverse transcriptase polymerase chain reaction (RT-PCR) and FGF2 secretion via ELISA. The effects of FGF signaling attenuation on OS cell pro-tumorigenic properties were evaluated by colorimetric proliferation assay and scratch migration assay. The effects of FGF signaling blockade with pan-FGFR inhibitor BGJ398 on OS cell differentiation were evaluated with Alizarin Red staining and quantification, alkaline phosphatase (ALP) bio-activity, and quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) for osteogenic genes alkaline phosphatase (ALP), osterix(OSTX), osteonectin (OSN), and runt-related transcription factor 2 (RUNX2). Circulating FGF2 levels were quantified in the plasma of dogs with naturally occurring OS (determined to be osteoblastic or osteolytic based on relative bone mineral density obtained via DEXA scan) and healthy controls via ELISA.
FGFR gene expression was noted in OS cell lines. FGF2 secretion was identified in all cell lines with secretion noted in a clear cell density-dependent manner in 2 of 3 cell lines. FGFR signaling attenuation inhibited OS cell migration while not affecting cell proliferation. FGFR signaling blockade increased differentiation in 1 of 2 cell lines evaluated, with a trend toward increased differentiation in the other cell line. The presence of naturally-occurring OS did not alter the level of circulating FGF2 in dogs. This study is the first to identify a link between FGF signaling attenuation and OS cell differentiation and migration in a cell line-dependent manner.
These findings indicate that FGF blockade could be beneficial in some dogs with OS.Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2018-03-13 without embargo termsThe student, Corrine Camero, accepted the attached license on 2017-10-22 at 14:08.The student, Corrine Camero, submitted this Thesis for approval on 2017-10-22 at 14:29.This Thesis was approved for publication on 2017-11-07 at 08:50.DSpace SAF Submission Ingestion Package generated from Vireo submission #11699 on 2018-03-13 at 10:08:01Made available in DSpace on 2018-03-13T15:45:08Z (GMT). No. of bitstreams: 2
CAMERO-THESIS-2017.pdf: 3286453 bytes, checksum: fc9dbf97d42ae13f14aca7970a612adf (MD5)
LICENSE.txt: 4211 bytes, checksum: 53706671144cbfdaa8d9c96403b33226 (MD5)
Previous issue date: 2017-11-0
Recombinant M protein-based ELISA test for infection of antibodies to canine coronavirus
No full textThe membrane (M) protein of canine coronavirus (CCoV) was cloned and expressed in E. coli. The purified recombinant protein was then evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for detection of CCoV antibodies in dog sera. Fifty serum samples, screened previously by whole virus ELISA and Western blotting, were tested. When the performance of the new test was compared with those of whole virus ELISA and Western blotting, an excellent correlation was found with the latter two assays. The ELISA based on recombinant M protein represents an alternative and valid test for detection of antibodies to CCoV in dog sera
Next‐generation sequencing analysis of root canal microbiota associated with a severe endodontic‐periodontal lesion
No full textA patient with an unusual endo‐periodontal lesion, without coronal decay or damage, likely caused by a deep periodontal lesion with subsequent endodontic bacterial migration, required medical care. Next‐generation sequencing (NGS) was used to assess the endodontic microbiota in vestibular and palatal canals after tooth extraction, evidencing a predominant population (Fusobacterium nucleatum) in one endodontic canal, and a mixed bacterial population with six major populations almost equally distributed in the other endodontic canal (F. nucleatum, Porphyromonas gingivalis, P. endodontis, Parvimonas, Peptostreptococcus stomatis, Prevotella multiformis). These data could suggest different, separated ecologic niches in the same endodontic system, with potentially different pathogenicity levels, clinical manifestations and prognoses for every single canal of the same tooth
Dr. Duane M. Jackson, Morehouse College, July 2011
No full textThis video is a conversation with Dr. Duane M. Jackson. Dr. Jackson talks about his paper, "Recall and the Serial Position Effect: The Role of Primacy and Recency on Accounting Students' Performance." Jackie Daniel, AUC Woodruff Library, is the interviewer
"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States" By M. Carey.
No full text"Reflections on the subject of Emigration from Europe with a view to Settlement in the United States: containing bried sketches of the moral and political character of those states.
By M. Carey, member of the American philosophical, and of the American Antiquarian Society, and author of The Olive Branch, Cindiciae Hibernicae, essays on banking, on political economy, and on internal improvement.
To which are now added the English editor's comments on the subject; together with Important Advice to Emigrants, and Cautions Against Impositions Practiced in the Outports
Assessment of a live attenuated glycoprotein E negative Bovine Herpesvirus 1 vaccine in goats
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