6,013 research outputs found
Development Of An On-line Adaptive ANN-based Controller For A Direct Expansion Air Conditioning System
No full textAn on-line adaptive artificial neural network (ANN)-based controller has been developed for an experimental DX A/C system. It controls the indoor air temperature and humidity simultaneously by varying the compressor speed and supply fan speed in a space served by the experimental DX A/C system. The ANN-based direct inverse control (DIC) strategy was adopted in the development of the controller and the specialized training method was used to on-line update an ANN-based model and an inverse model used in the controller. The controllability tests including the command following test and the disturbance rejection test were carried out using the experimental DX A/C system, and the test results showed that the on-line adaptive ANN-based controller developed was able to control indoor air dry-bulb temperature and wet-bulb temperature outside the operating conditions within which the models were trained, with a high control accuracy
DX mutant phenotypes.
No full textStrains were stained with Calcofluor White after 4 hr growth at 30°C in YPD medium. Expression levels of core filamentation genes in mutants grown for 4 hr at 30° in YPD are expressed as fold-change to the wild type; complete data are in S2 Table. Strains: WT (DAY286), cln3 DX (CW994), cak1 DX (CW1003), kin28 DX (CW1041), gin4 DX (CW900), dbf2 DX (CW914), ctk1 DX (CW1005), ipl1 DX (CW1038), snf1 DX (CW927), sak1 DX (CW995), ire1 DX (CW906), mck1 DX (CW1006), cdc28 DX (CW991), cmk2 DX (CW999), cdc7 DX (CW993), sha3 DX (CW1010).</p
Dynamic state feedback decoupling of a DX A/C system
No full textA temperature and humidity controller is designed for a direct expansion air conditioning (DX A/C) system making use of a state feedback decoupling approach of nonlinear control systems. It is shown that the nonlinear dynamics of a DX A/C system can be input-output decoupled by dynamic state feedback. The resulting decoupled system is of minimum phase. Thereafter, the decoupled model is used to design a pole placement controller with guaranteed stability. Unlike controllers based on approximate local linearization of the DX A/C model, the controller proposed is global in the sense that it can track temperature and humidity setpoints in the complete operating range of the DX A/C system. Effectiveness of the controller designed is demonstrated by simulation results.https://www.journals.elsevier.com/ifac-papersonlineam2020Electrical, Electronic and Computer Engineerin
DX mutant complementation.
No full textA. Cell morphology. Strains were stained with Calcofluor White after 4 hr growth at 30°C in YPD medium. Strains: cak1 DX (CW1428), cak1 DX comp (CW1431), cln3 DX (CW1437), cln3 DX comp (CW1440), kin28 DX (CW1082), kin28 DX comp (CW1085), ctk1 DX (CW1076), ctk1 DX comp (CW1078), snf1 DX (CW1138), snf1 DX comp (CW1141). B. Cek1 phosphorylation. Immunoblots were performed on yeast extracts from mid-log phase cells grown for 5 hr at 30°C. Strains for lanes 1, 3, 6, 8, 11, 12, 15, and 18 are His-; strains for lanes 2, 4, 5, 7, 9, 10, 13, 14, 16, 17, and 19 are His+. The lanes show experiments with the strain in parentheses: 1 (DAY286), 2 (CW1078), 3 (CW1005), 4 (CW1075), 5 (CW1076), 6 (TA72), 7 (TA86), 8 (CW1041), 9 (CW1082), 10 (CW1085), 11 (DAY286), 12 (CW927), 13 (CW1138), 14 (CW1141), 15 (CW995), 16 (CW1111), 17 (CW1114), 18 (TA72), 19 (TA86). C. Gene expression profiles. RNA was extracted from cells grown for 4 hr at 30°C in YPD and used for nanoString expression analysis (S3 Table). Hierarchal clustering of gene expression data was performed using MeV software. Fold change values were obtained by dividing normalized expression values for each mutant or complemented strain by the wild type strain (DAY185) for each of the probes. The color scale represents Log2 fold change compared to wild type. (Blue limit: 10-fold down; yellow limit: 10-fold up.) Strains are listed in S3 Table.</p
Accumulation of C-terminal fragments of transactive response DNA-binding protein 43 leads to synaptic loss and cognitive deficits in human TDP-43 transgenic mice
No full textAccumulation of the transactive response DNA-binding protein 43 (TDP-43) is a major hallmark of several neurodegenerative disorders, collectively known as TDP-43 proteinopathies. The most common TDP-43 proteinopathies, frontotemporal lobar degeneration with TDP-43-positive inclusions, and amyotrophic lateral sclerosis, share overlapping neuropathological and clinical phenotypes. The development and detailed analysis of animal models of TDP-43 proteinopathies are critical for understanding the pathogenesis of these disorders. Transgenic mice overexpressing mutant human TDP-43 (herein referred to as hTDP-43) are characterized by neurodegeneration and reduced life span. However, little is known about the behavioral phenotype of these mice. Here we report the novel finding that hTDP-43 mice develop deficits in cognition, motor performance, and coordination. We show that these behavioral deficits are associated with the accumulation of nuclear and cytosolic TDP-43 C-terminal fragments, a decrease in endogenous TDP-43 levels, and synaptic loss. Our findings provide critical insights into disease pathology, and will help guide future preclinical studies aimed at testing the effects of potential therapeutic agents on the onset and progression of TDP-43 proteinopathies. (C) 2014 Elsevier Inc. All rights reserved
DX-like centers in n-type Al-doped ZnS1-xTex grown by molecular-beam epitaxy
No full textAl-related DX-like centers were observed in n-type Al-doped ZnS1-xTex epilayers grown by molecular-beam epitaxy on GaAs substrates. The capacitance-voltage measurement, deep-level transient spectroscopy, and photoconductivity spectroscopy revealed that the behaviors of Al donors in ZnS1-xTex were similar to the so-called DX centers in AlxGa1-xAs. The optical ionization energies (E-i) and emission barriers (E-e) for the observed two Al-related DX-like centers were determined as E-i similar to 1.0 and 2.0cV and E-e similar to 0.21 and 0.39 eV, respectively. It was also shown that the formation of Al-related DX-like centers resulted in a significantly large lattice relaxation in ZnS1-xTex. (C) 2000 Elsevier Science B.V. All rights reserved
Glucocorticoids Exacerbate Cognitive Deficits in TDP-25 Transgenic Mice via a Glutathione-Mediated Mechanism: Implications for Aging, Stress and TDP-43 Proteinopathies
No full textThe accumulation of TDP-43 (transactive response DNA-binding protein 43) and its 25 kDa C-terminal fragment (TDP-25) is a hallmark of several neurodegenerative disorders, including frontotemporal lobar degeneration (FTLD-TDP) and amyotrophic lateral sclerosis (ALS). The majority of FTLD-TDP cases are due to loss of function mutations in the gene encoding progranulin, a secreted growth factor. In ALS, specific mutations in the gene encoding TDP-43 have been linked to the disease pathogenesis. In both cases, however, the penetrance of the mutations greatly increases during aging, suggesting that other genetic or environmental factors may facilitate the development of the disease. Using transgenic mice that overexpress the 25 kDa C-terminal fragment of TDP-43, here we show that glucocorticoids, stress hormones known to increase the brain susceptibility to neurotoxic insults, increase the levels of soluble TDP-25 and exacerbate cognitive deficits, without altering full-length TDP-43 levels. Additionally, we show that the mechanism underlying the glucocorticoid-mediated increase in TDP-25 levels is coupled to changes in the glutathione redox state. Glutathione is an antioxidant involved in protecting cells from damage caused by reactive oxygen species; notably, alterations in the ratio of reduced to oxidized glutathione, which is the primary determinant of the cellular redox state, are associated with aging and neurodegeneration. We show that restoring the ratio of reduced to oxidized glutathione blocks the glucocorticoid effects on TDP-25. These data show that glucocorticoids potentiate the neurotoxic action of TDP-25 by increasing its levels and clearly indicate the role of cellular oxidative damage in this process
Quantitative analysis of chloroplast protein targeting
No full textThis thesis presents the first use of the Partition of Unity Method in quantifying
the spatio-temporal dynamics of a fluorescent protein targeted to
the chloroplast twin-arginine translocation pathway.
The fluorescence loss in photobleaching technique is applied in a modified
fashion to the measurement of substrate mobilities in the chloroplast
stroma. Our in vivo results address the two suggested protein targeting
mechanisms of membrane-binding before lateral movement to the translocon
and direct binding to the translocon.
A high performance computing C/C++ implementation of the Partition
of Unity Method is used to perform simulations of fluoresence loss
in photobleaching and allow a compelling comparison to photobleaching
data series. The implementation is both mesh-free and particle-less
Hot electron and DX center insensitivity of Al0.25Ga0.75As/GaAs HFET’s designed for microwave power applications
No full textWe show in this work that, although designing AlxGa1-xAs/GaAs HFET's for microwave power applications requires a large barrier layer bandgap (hence x>0.2), the presence of a large concentration of electrically active DX centers in the barrier layer does not hinder the device reliability. The existence of a remarkable quantity of DX centers in the Al0.25Ga0.75As barrier layer is for the first time revealed by means of room temperature electroluminescence, and their concentration is evaluated by measuring the threshold voltage shift induced by hot electron stress at cryogenic temperature
Methods for the Determination of Z and M Using dE/dx, Čerenkov, and Total Energy Measurements
No full textThe current status of mass (M) and charge (Z) measurements are reviewed for cosmic ray nuclides, and an estimation is made of their possible status by 1980. The determination of M using dE/dx-E and C.E techniques is discussed, and typical instrument characteristics are described. The determination of Z is discussed for ultraheavy cosmic rays using the dE/dx-C technique
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