115,913 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
No full textThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
The C+CH Reaction on 2 Electronic states
No full textThe influence of electronically nonadiabatic transitions in the C+CH reaction on the ground and first excited states is investigated by using time-dependent wave packet method. With initial wave packet on one single potential energy surface, and the diatomic CH having different internal states, we obtain the total reaction probabilities of the 2 electronic states respectively for the total angular momentum J=0. Calculations for J>0 are currently running, as they are so time consuming
INFRARED AND RAMAN SPECTRA OF , AND
No full textAuthor Institution: Army Natick Labs.; Mellon Institute, PittsburghInfrared spectra of , and have been measured from 33 to for the vapor and for solutions in several solvents. Raman spectra with polarizations were obtained for solutions. symmetry was assumed and was completely satisfactory. Fundamentals for species and could be assigned with little difficulty, but only a few of the Raman active e fundamentals were observed
"C??i ch???t, Ph???t gi??o v?? ch??? ngh??a hi???n sinh trong nh???c Tr???nh C??ng S??n"
No full text???? c?? r???t nhi???u l???i gi???i th??ch v??? s??? n???i ti???ng c???a ca nh???c s?? Tr???nh C??ng S??n qua nh???ng ????? t??i nh??: ca t??? ?????y ch???t th??, kh??c v???i d??ng nh???c ti???n chi???n, mang ch??? ????? ph???n chi???n, v?? c??? vi???c ??ng ???? kh??m ph?? ra nh???ng ti???ng h??t n??? t??i n??ng, v?? c??n nhi???u ??i???u kh??c n???a. Nh??ng ch??? ????? Ph???t gi??o trong nh???ng b??i h??t c???a ??ng l???i r???t ??t khi ???????c nh???c ?????n, ph???i ch??ng, ????y l?? ??i???u nh???ng h???c gi??? Vi???t Nam cho l?? hi???n nhi??n. B??i vi???t n??y n??i ?????n ch??? ????? Ph???t gi??o trong nh???c Tr???nh v?? ch???ng minh r???ng ch??? ????? n??y g??p ph???n v??o vi???c gi???i th??ch hi???n t?????ng Tr???nh C??ng S??n. Ngo??i ra, b??i vi???t n??y c??ng ????? c???p ?????n ch??? ngh??a hi???n sinh ??u ch??u, l?? ??i???u m?? t??c gi??? b??i vi???t cho r???ng ???? thu h??t Tr???nh C??ng S??n nh??ng kh??ng c?? ???nh h?????ng l???n ?????n nh???ng s??ng t??c c???a ??ng
Growth of RA-CH-1pLMF03, RA-CH-1<i>ΔB739_1343</i>pLMF03, and RA-CH-1<i>ΔB739_1343</i>pLMF03::<i>B739_1343</i> on TSA and TSA supplemented with 50 μM Dip.
No full textThe R. anatipestifer strains (clockwise from top left) RA-CH-1pLMF03, RA-CH-1ΔB739_1343pLMF03, and RA-CH-1ΔB739_1343pLMF03::B739-1343 were grown on TSA plates containing cefoxitin (1 μg/mL) and 0 μM Dip (A) or 50 μM Dip (B). Growth was assessed by the appearance of bacterial colonies on plates. Pictures were taken after 48 h of growth at 37°C. All the experiments were repeated three times. Representative plates are presented.</p
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Using δ¹³C-CH₄ and δD-CH₄ to constrain Arctic methane emissions
No full text© Author(s) 2016.We present a global methane modelling study assessing the sensitivity of Arctic atmospheric CH₄ mole fractions, δ¹³C-CH₄ and δD-CH₄ to uncertainties in Arctic methane sources. Model simulations include methane tracers tagged by source and isotopic composition and are compared with atmospheric data at four northern high-latitude measurement sites. We find the model's ability to capture the magnitude and phase of observed seasonal cycles of CH₄ mixing ratios, δ¹³C-CH₄ and δD-CH₄ at northern high latitudes is much improved using a later spring kick-off and autumn decline in northern high-latitude wetland emissions than predicted by most process models. Results from our model simulations indicate that recent predictions of large methane emissions from thawing submarine permafrost in the East Siberian Arctic Shelf region could only be reconciled with global-scale atmospheric observations by making large adjustments to high-latitude anthropogenic or wetland emission inventories
Preface of 2nd workskhop on advanced visual interfaces for cultural heritage 2018 (AVI-CH 2018)
No full textPreface of 2nd workskhop on advanced visual interfaces for cultural heritage 2018 (AVI-CH 2018
COUPLING OF THE C-H STRETCH TO LARGE-AMPLITUDE TORSION AND INVERSION MOTIONS: COMPARISON OF CHCH, CHOH AND CHNH
No full textAuthor Institution: Department of Polymer Science and Department of Chemistry, The University of Akron; Department of Chemistry, The University of Akron, OH 44325In each of the title molecules, torsional and inversion tunneling occurs between six equivalent minima. Coupling of these degrees of freedom to the CH stretch occurs via variation of the C-H stretching force constants as a function of the torsional () and inversion () angles. Maps of the couplings have been computed at the MP2/6-311++G(3df,2p) level. Both the single bond CH stretch force constants and the bilinear couplings between CH bonds are presented as a function of and . Although the torsional barriers differ by more than a factor of 20, the torsion-inversion-vibration coupling patterns are very similar for CHNH and CHCH. On the other hand, the torsion-inversion-vibration coupling in the charged species CHOH is much weaker
Kinetics and mechanism of the reactions of 2,3-butadione with F and Cl atoms, UV absorption spectra of CH<sub>3</sub>C(O)C(O)CH<sub>2</sub> · and CH<sub>3</sub>C(O)C(O)CH<sub>2</sub>O<sub>2</sub> · radicals, and atmospheric fate of CH<sub>3</sub>C(O)C(O)CH<sub>2</sub>O · radicals
No full text FTIR-smog chamber techniques were used to determine rate constants for the gas-phase reaction of Cl and F atoms with CH 3 C(O)C(O)CH 3 and F atoms with CH 3 C(O)F of (4.0 ± 0.5) x 10 -3 , (4.9 ± 0.7) x 10 -11 , and (3.6 ± 0.4) x 10 -12 cm 3 molecule -1 s -1 , respectively. Two pathways for the reaction of Cl and F atoms with CH 3 C(O)C(O)CH 3 were found: (1a) Cl + CH 3 C(O)C(O)CH 3 → HCl + CH 3 C(O)C(O)CH 2 ·, (1b) Cl + CH 3 C(O)C(O)CH 3 → CH 3 C(O)Cl + CH 3 C(O)·, (2a) F + CH 3 C(O)C(O)CH 3 → HF + CH 3 C(O)C(O)CH 2 ·, (2b) F + CH 3 C(O)C(O)CH 3 → CH 3 C(O)F + CH 3 C(O)·, with branching ratios of k 1b /(k 1b + k 1a ) = 0.23 ± 0.02 and k 2b /(k 2b + k 2a ) = 0.56 ± 0.09. It was determined that the atmospheric fate of CH 3 C(O)C(O)CH 2 O· radicals is decomposition to give HCHO, CO, and CH 3 C(O)· radicals. Pulse radiolysis coupled to UV absorption spectroscopy was used to study the kinetics of the reaction of F atoms with CH 3 C(O)C(O)CH 3 as well as spectra of CH 3 C(O)C(O)CH 2 · and CH 3 C(O)C(O)CH 2 O 2 · radicals over the wavelength range 220-400 nm at 295 K. The rate constant for the reaction of F atoms with CH 3 C(O)C(O)CH 3 was determined to be (4.6 ± 0.8) x 10 -11 cm 3 molecule -1 s -1 . The absorption cross sections of CH 3 C(O)C(O)CH 2 and CH 3 C-(O)C(O)CH 2 O 2 · radicals were (5.4 ± 1.0) x 10 -18 at 250 nm and (2.0 ± 0.5) x 10 -18 cm 2 molecule -1 at 320 nm, respectively. Results are discussed with respect to the available database concerning the reaction of Cl and F atoms with organic compounds. </p
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The combination reaction of CH{sub 3} and C{sub 6}H{sub 5}O
No full textKinetics and mechanism of the CH{sub 3} + C{sub 6}H{sub 5}O reaction have been examined by means of the RRKM theory and kinetic modeling. Both the high temperature shock tube data for the production of cresols and the low temperature branching ratios (for production of cresols vs. methylcyclohexadienones, CH{sub 3}C{sub 6}H{sub 5}O) derived from Mulcahy and William`s data on the pyrolysis of di-t-butyl peroxide and phenol mixtures (Ref. 1) could be reasonably accounted for by the mechanism: CH{sub 3} + C{sub 6}H{sub 5}O {yields} CH{sub 3}C{sub 6}H{sub 5}O{sup +} {yields} o- and p-CH{sub 3}C{sub 6}H{sub 4}OH +M {yields} CH{sub 3}C{sub 6}H{sub 5}O. The energy barrier for the thermal iosmerization of CH{sub 3}C{sub 6}H{sub 5}O to cresols was estimated to be {approximately}132 kJ mol{sup {minus}1}
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