3,824 research outputs found
Stam. C. Caratzas, Les Tzacones
No full textMawet Francine. Stam. C. Caratzas, Les Tzacones. In: L'antiquité classique, Tome 48, fasc. 1, 1979. p. 342
Stam. C. Caratzas, Les Tzacones
No full textIrigoin Jean. Stam. C. Caratzas, Les Tzacones. In: Bulletin de l'Association Guillaume Budé, n°2, juin 1978. pp. 215-216
Stam. C. Caratzas, Les Tzacones
No full textMawet Francine. Stam. C. Caratzas, Les Tzacones. In: L'antiquité classique, Tome 48, fasc. 1, 1979. p. 342
The genetic basis of chronic myelogenous and acute lymphoblastic leukemia
No full textThe Ph' chromosome is a cardinal feature of chronic myelogenous leukemia (CML). Originally described by Nowell and Hungerford (1960) as a small chromosome 22 it was later demonstrated by de Klein and co-workers(1982) to typically result from a reciprocal translocation t(9;22)(q34.1;q11.2). In approximately 5 percent of patients, the Ph' chromosome results from anomalous complex translocation. Detailed studies using in situ hybridization have demonstrated that chromosomes 9 and 22 are usually involved in such translocations. Translocations between chromosome 22 and a chromosome other than 9 rarely occur.The human c-abl sequences represent the cellular homologue of the transforming gene of Abelson Murine Leukemia Virus (A-MuLV). This retrovirus is a recombinant between Moloney Murine Leukemia virus and mouse cellular c-abl sequences (Goff et al., 1980). A-MuLV induces lymphoid tumors following invivo inoculation of the mouse (Potter, 1983; Prekumar et al., 1975). Southern blot analysis of a series of somatic cell hybrids demonstrated that the human c-abl gene is localized on chromosome 9 (Heisterkamp et al., 1982). The finding that a small region of chromosome 9 is translocated to chromosome 22 in CML prompted studies to elucidate whether the abl gene was involved in this disease (de Klein et al., 1982). Bartram and colleagues (1983) first reported that the c-abl gene is translocated in Ph' positive but not in Ph' negative patients while Heisterkamp and co-workers (1983) reported a CML patient with a breakpoint 14 kb 5' of the c-abl sequences homologous to v-abl. This data suggested a role for c- abl in CML, a theory supported by the presence of an abnormally sized abl messenger RNA (Collins et al., 1984; Gale and Canaani, 1984) and abl protein in the CML, cell line K562 (Konopka et al., 1984).The region of chromosome 22 involved in the translocation has also been identified. Cloning of a chimeric breakpoint fragment from a CML, patient (Heisterkamp et al., 1983) enabled the use of a chromosome 22 specific probe. Subsequent Southern blot analysis of the DNA of a number of CML, patients showed that chromosome 22 breakpoints map to a stretch of 5.8 kb of DNA (Groffen, et al., 1984). This area on chromosome 22 was designated breakpoint cluster region or bcr.The investigation described in this thesis was undertaken to gain further insight into the genetic organization of the c-abl gene on the Ph' chromosome and the consequence of the Ph' translocation at the transcription and translation level. Chapter 2 is an extensive review of the genetic basis of CML, and acute lymphoblastic leukemia (All). Discussed is the cytogenetic and molecular aspects of CML, and ALL and the activation of the c-abl protein kinases as a consequence of the Ph' translocation. In Chapter 3, we show that the breakpoint cluster region on chromosome 22 is part of a gene, called phl . Molecular characterization demonstrates that phl . is a large gene oriented with its 5' end towards the centromere and with its 3' end toward the telomere of chromosome 22. As a consequence of the Ph' translocation the 3' end of this gene is translocated to chromosome 9, whereas the S' sequences remain on the Ph' chromosome. Following translocation, the remaining phl sequences become fused to the c-abl gene in a head to tail fashion on chromosome 22. We hypothesized that this genomicconfiguration could result in the transcription of a chimeric mRNA consisting of 5' phl and 3' abl sequences. In the study presented in Chapter 4, we analyzed the RNA of CML patients and found strong evidence for this model. Direct proof for the hypothesis was provided by cloning of a chimeric phl /c- abl cDNA in the CML cell line K562, described in Chapter 5. The experiments described herein have also contributed to more information about the genomic organization of the phl. and c-abl genes on the Ph' chromosome.Reports by Konopka et al. (1985) showing the presence of a larger 210K c- abl protein in the leukemic cells of CML patients initiated the studies described in Chapter 6. We demonstrate that this abnormal c- abl protein is a fusion protein containing amino terminal phl and carboxy terminal abl sequences. This phl /c- abl protein has an elevated tyrosine kinase activity when compared to the normal c- abl protein. In addition, we show in this chapter that the normal phl. gene encodes for a 160 K phosphoprotein exhibiting an associated kinase activity.A better understanding of the Ph' chromosome at the molecular level has allowed us to develop a probe for the detection of the Ph' translocation. In Chapter 7, we report the results of the clinical trials done at seven medical centers involving more than 400 clinical samples. In this report we demonstrate that the use of DNA probe analysis in the CML diagnostics has several advantages over cytogenetic methods
I don't think we are alone; interview
No full textThere are scores of earth-like planets outside our solar system. Dr Daphne Stam (faculty of Aerospace Engineering) wants to launch a space telescope to search for life on these planets.Space EngineeringAerospace Engineerin
De merkwaardige geschiedenis van de stoel van Stam
No full textEen spannend verhaal over de merkwaardige en gecompliceerde geschiedenis van de achterpootloze stoel, Mart Stam, Marcel Breuer, Breuer-Stam hybriden, auteursrechten en licenties.ArchitectureArchitecture and The Built Environmen
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Functional mapping of the sensorimotor cortex: Clinical studies with MEG and fMRI
No full textVandertop, W.P. [Promotor]Stam, C.J. [Promotor
<i>argos</i> expression is strongly reduced in <i>stam</i>, <i>hrs</i> and <i>hrs, stam</i> mutant cells in pupal wings.
No full text<p><i>argos</i> expression was analysed in <i>wild type</i> (A), <i>stam<sup>2L2896</sup></i> (B) and <i>hrs, stam</i> (C) MARCM clones visualized with mCD8-GFP (green) in pupal wings (24–30 hrs APF) using a <i>argos</i>-<i>LacZ</i> enhancer trap line. White dotted squares (in A–C) indicate the position of close-up pictures. White doted lines (in A′–C′) indicated the position of the clones in the close-up pictures. The <i>argos</i> staining (red) is absent in stam and <i>hrs, stam</i> mutant cells.</p
Central Station and issues of identity in film form and critical debates
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-graduação em Letras/Inglês e Literatura Correspondente, Florianópolis, 2009O presente trabalho trata de questões relacionadas à construção de uma identidade cultural brasileira no filme Central do Brasil, dirigido por Walter Salles, a partir da perspectiva fílmica e também relacionada aos debates críticos que o filme gerou em resenhas americanas e brasileiras. Argumenta-se que o filme é uma metáfora da busca por identidade, argumento usado pelo próprio diretor. Esta busca se dá no nível pessoal, no caso das personagens principais, Dora e Josué, e nacional, já que o filme encoraja a associação da jornada das personagens a uma busca pela identidade nacional brasileira. A pesquisa buscou encontrar os elementos fílmicos que remetem a essa hipótese de que o filme é uma metáfora da busca por identidade, bem como de que forma (e se) os debates críticos gerados a partir do filme identificaram essa questão da busca. Para teorias de identidade, autores como Stuart Hall, Zygmunt Bauman e Robert Stam foram usados. Para teoria fílmica foram usados trabalhos de David Bordwell e Marcel Martin. Para estudos em cinema brasileiro, leituras de trabalhos de Luiz Zanin Oricchio, Robert Stam e Sidney Ferreira Leite foram realizadas. A análise mostrou que o filme, de fato, traz elementos que podem ser relacionados à metáfora da busca por identidade.This research addresses questions related to the construction of a Brazilian cultural identity in the film Central Station, directed by Walter Salles, from the perspective of film form, and concerning the critical debates prompted by the film in Brazilian and American reviews. The film is argued to be, by the director himself, a metaphor for the search for identity, both personal, in the case on the main characters, Dora and Josué, and national, since the film encourages the association of the characters' journey to a search for a Brazilian identity. What this research sought to find were the elements in film form that relate to this hypothesis of the film as a metaphor for the search of identity, and how and if the critical debates prompted by the film identified the issue of identity. For the issue of identity, critics such as Stuart Hall, Robert Stam, and Zygmunt Bauman were invoked. For Film Studies, theorists such as Bordwell and Marcel Martin were used. For readings in Brazilian Cinema, the works of Luiz Zanin Oricchio, Robert Stam and Sidney Ferreira Leite were used. The analysis showed that the film, indeed, brings elements that can be related to a metaphor for the search for identity
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