61 research outputs found
Global analysis of gene expression identifies C/EBP and TEF-1 as major p38 alpha MAP kinase-regulated transcription factors in proliferating cardiomyocytes.
No full textp38 MAPKs (mitogen-activated protein kinases) play important
roles in the regulation of cellular responses to environmental
stress. Recently, this signalling pathway has also been implicated
in the regulation of processes unrelated to stress, for example, in
T lymphocytes and cardiomyocytes. In order to identify molecular
targets responsible for the housekeeping functions of p38
MAPKs, we have analysed the differences in the transcriptomes
of normally proliferating wild-type and p38α knockout immortalized
embryonic cardiomyocytes. Interestingly, many potential components of the myocardium extracellular matrix were found
to be upregulated in the absence of p38α. Further analysis of
the microarray data identified TEF-1 (transcriptional enhancer
factor-1), a known regulator of heart-specific gene expression,
and C/EBPβ (CCAAT/enhancer-binding protein β), as the two
transcription factors the binding sites ofwhich were most enriched
in the promoters of p38α-regulated genes. We have focused on
the study of the extracellular matrix component COL1A1 (α1
chain of type I collagen) and found evidence for the involvement
of both TEF-1 and C/EBPβ in the p38α-dependent inhibition of
COL1A1 transcription. Our data therefore show that p38 MAPKs
regulate TEF-1 andC/EBPβ transcriptional activity in the absence
of environmental stress and suggests a role for p38α in the expression of extracellular matrix components that maintain organ
architecture
Review of: The inhibitor of cyclin-dependent kinase 4a/alternative reading frame (INK4a/ARF) locus encoded proteins p16INK4a and p19ARF repress cyclin D1 transcription through distinct cis elements
No full textCommentary of the article: D’Amico M., Wu K., Fu M., Rao M., Albanese C., Russell R. G., Lian H., Bregman D., White M. A., Pestell R. G. Cancer Research 2004 Jun 15; 64(12): 4122–4130
Analysis of the estrogen-responsive transcriptome from breast cancer cells: a comparative analysis with three different microarray platforms.
No full texthttp://www.nettab.org/2003
Identification of the estrogen-responsive transcriptome from human breast cancer cells: a comparative analysis with four different microarray platforms.
No full textProceedings of the Illumina User Group meeting 2006. 12-14 March 2006. San Diego (USA
A combinatorial approach to gene expression analysis: DNA microarrays.
No full textThe microarray technology is based on analytical tools that parallelize the quantitative and qualitative analysis of nucleic acids, proteins and tissue sections one of its more recent evolutions-. By miniaturizing the size of the reaction and sensing area, microarrays allow to assess at the activity of thousands of genes in a given tissue or cell line at once in a rapid and quantitative way, and to carry out serial comparative tests in multiple samples. These tools, that stem from the innovations resulting from the technological improvements and knowledge arising from the genome sequencing projects, can be considered as a combinatorial technique that can rapidly provide significant information about complex cellular pathways and processes within one or few ‘‘mass scale’’ and comprehensive testing of a biological sample’s composition
Lo strano caso del vivente uomo: la narrazione biologica tra nuovi miti e antiche nostalgie
No full textil saggio analizza il passaggio dalla tradizionale narrazione di matrice umanistica, che intende l'umano come prodotto inedito e trascendente le leggi della natura, alla biologia contemporanea che, ricollocandolo orizzontalmente all'interno del mondo naturale, va producendo su di esso una narrazione inedita ma non per questo meno interessant
Molecular mechanisms of female sex hormone action on proliferation.
No full textProceedings of the EMBO Conference "Nuclear Receptors: From Chromatin to Disease". 29 Sept-1 October 2005. Gardone Riviera, BS (Italy
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