145 research outputs found
Miglioramento delle rese nelle sintesi di plasteina con enzimi immobilizzati
No full textLe frazioni dializzabile di succhi deproteinati di erba medica preparati poco dopo (70 min) la falciatura delle piante e quella di analoghi succhi preparati dopo 14 ore a 20 °C, sono state impiegate come substrato per la sintesi plasteinica con alfa-chimotripsina immobilizzata su chitina
The claustrum of the bottlenose dolphin Tursiops truncatus (Montagu 1821)
No full textThe mammalian claustrum is involved in processing sensory information from the environment. The claustrum is reciprocally connected to the visual cortex and these projections, at least in carnivores, display a clear retinotopic distribution. The visual cortex of dolphins occupies a position strikingly different from that of land mammals. Whether the reshaping of the functional areas of the cortex of cetaceans involves also modifications of the claustral projections remains hitherto unanswered. The present topographic and immunohistochemical study is based on the brains of eight bottlenose dolphins and a wide array of antisera against: calcium-binding proteins (CBPs) parvalbumin (PV), calretinin (CR), and calbindin (CB); somatostatin (SOM); neuropeptide Y (NPY); and the potential claustral marker Gng2. Our observations confirmed the general topography of the mammalian claustrum also in the bottlenose dolphin, although (a) the reduction of the piriform lobe modifies the ventral relationships of the claustrum with the cortex, and (b) the rotation of the telencephalon along the transverse axis, accompanied by the reduction of the antero-posterior length of the brain, apparently moves the claustrum more rostrally. We observed a strong presence of CR-immunoreactive (-ir) neurons and fibers, a diffuse but weak expression of CB-ir elements and virtually no PV immunostaining. This latter finding agrees with studies that report that PV-ir elements are rare in the visual cortex of the same species. NPY- and somatostatin-containing neurons were evident, while the potential claustral markers Gng2 was not identified in the sections, but no explanation for its absence is currently available. Although no data are available on the projections to and from the claustrum in cetaceans, our results suggest that its neurochemical organization is compatible with the presence of noteworthy cortical inputs and outputs and a persistent role in the general processing of the relative information. © 2014 Cozzi, Roncon, Granato, Giurisato, Castagna, Peruffo, Panin, Ballarin, Montelli and Pirone
Atwo-dimensional electrophoretic method for the study of the caprine alpha S1 and alpha S2 casein polymorphism
No full textParziale purificazione e caratterizzazione dell'aminopeptidasi dell'uva
No full textL'aminopeptidasi dell'uva è stata purificata 21 volte mediante frazionamento con solfato d'ammonio, gel filtrazione su sephadex G-200 e cromatografia su colonna di DEAE-Sephadex.
Le proprietà dell'aminopeptidasi sono discusse con riferimento all'ipotesi secondo la quale le proteasi dell'uva possono catalizzare una parziale idrolisi delle proteine della stessa nel corso della vinificazione
Recupero mediante reazione plasteinica di peptidi da succhi deproteinati di erba medica
No full textE' stato condotto uno studio tendente a definire le condizioni per una efficiente sintesi di plasteina con enzimi immobilizzati
To Go Digital or Not to Go Digital? The Influence of Board’s Digital Expertise on Strategic Change of The Firm
No full textPlexcitonic Nanohybrids Based on Gold Nanourchins: The Role of the Capping Layer
No full textPlexcitonic nanohybrids are plasmonic-excitonic novel materials whose peculiar properties are attracting considerable attention in photonics, solar cells, and sensing. These materials can be synthesized and characterized easily by assembling organic or inorganic dyes on plasmonic nanoparticles as support. However, the main factors controlling the assembly process and the occurrence of the plexcitonic coupling are still unclear. To fill this gap, in this work, we studied the plexciton coupling of 12 different dyes with a series of gold nanourchins with various coatings and sizes. Among 24 combinations tested, we observed the formation of plexcitonic hybrids only in five cases. Most of them had cyanine J-aggregates as excitonic counterparts. Stronger plexcitonic couplings were obtained when nanourchins were coated with an exchangeable citrate capping layer rather than a strong ionic thiols capping layer. We propose that the presence of a strongly bound capping layer, as in this latter ca..
To Go Digital or Not to Go Digital? The Influence of Digital Expertise of The Board of Directors on (Digital) Strategic Change
No full textThe strategic change is considered a key issue in the fields of management and strategy. We aim at investigating the impact of digital expertise of board of directors on digital strategic change. We expect that the inclusion board of directors with digital-related experience positively influence the initiation and the implementation of strategic change. We reconcile the upper echelons theory with the resource dependence theory by understanding under which conditions the board is responsible for directing the strategic change in the context of digital transformation
Plastic additive di(2-ethylhexyl)phthalate (DEHP) causes cell death and micronucleus induction on a bottlenose dolphin's (Tursiops truncatus) in vitro-exposed skin cell line
No full textMarine plastic pollution is one of the most concerning worldwide environmental issues, and research is day by day demonstrating its adverse effects on marine ecosystems. Nevertheless, little is still known about the toxic potential on marine fauna of chemical additives released by plastic debris. Here we investigated the cyto- and genotoxicity of the most used plasticizer in plastic production, di(2-ethylhexyl)phthalate (DEHP), on a skin cell line (TT) derived from the bottlenose dolphin (Tursiops truncatus), a species particularly exposed to the accumulation of this lipophilic pollutant, being a coastal top predator rich in fatty subcutaneous tissues. Dolphin cell cultures were exposed to increasing DEHP doses (0.01-5 mM) to evaluate effects on cell viability, cell death, and induction of DNA damage. On the hypothesis that bottlenose dolphin cells show greater resistance to DEHP toxicity than terrestrial mammals, as already shown for other pollutants, the same parameters were analyzed on exposed Chinese hamster ovary (CHO) cell lines. Both MTT and Trypan Blue assays showed no significant decrease in dolphin's cell viability after 24-h DEHP exposure. No induction of primary DNA damage was detected by the comet assay, whereas the cytokinesis-block micronucleus assay revealed significant micronuclei induction and inhibition of cell proliferation starting from the lowest DEHP doses. DEHP had similar but sharper and significant effects on cell viability in CHO cells, also causing a much greater induction of necrosis than that recorded on dolphin cells. For both cell lines, the lack of induction of primary DNA damage (i.e., strand breaks) together with the increase of micronuclei yield after DEHP treatment suggests an aneugenic effect of the phthalate, that is, the loss of entire chromosomes during cell division. Overall, the potential chromosome loss detected could constitute a threat for species of marine mammals constantly exposed to plastic marine litter
Immobilisation of rice limit dextrinase on gamma-alumina beads and its possible use in starch processing
No full textLimit dextrinase (EC 3•2•1•41) partially purified from rice seeds was immobilized by adsorption on γ-alumina beads. Binding and activity yields were 88 and 52%, respectively. The adsorbed enzyme showed a broader pH optimum and an increased thermal stability compared to the free one. Optimum temperature was between 50 and 60°C and the enzyme retained about 70% of the initial activity after 7 days of incubation at 40°C in the presence of Ca2+. The immobilized biocatalyst degraded pullulan completely to maltotriose and effectively converted both acid hydrolysed amylodextrins and β-limit dextrins into maltose and maltotriose
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