117 research outputs found
Il sirventese «Felon cor ai et enic» di Percivalle Doria
No full textIl contributo propone edizione e studio del sirventese Felon cor ai et enic (1258-1259), in cui Percivalle Doria mostra due aspetti salienti della sua personalità e della sua vita: il coinvolgimento politico e l’impegno poetico. L’autore elogia re Manfredi di Svevia e gli offre il suo sostegno nelle lotte per il potere in cui è implicato.
The essay offers an edition and analysis of the sirventes Felon cor ai et enic (1258-1259), where Percivalle Doria shows two distinguishing aspects of his personality and life: political participation and poetical care. The author praises king Manfredi and gives to him his support in the fights for power in which he is implicated
Guido Delle Colonne, Re Giovanni, Arrigo Testa, Ruggerone da Palermo, Ruggieri Apugliese, Re Enzo, Percivalle Doria
No full textEdizione critica e commentata dei seguenti poeti della Scuola siciliana: Guido Delle Colonne, Re Giovanni, Arrigo Testa, Ruggerone da Palermo, Ruggieri Apugliese, Re Enzo, Percivalle Doria. L'edizione fa parte dell'opera in tre volumi dei Meridiani Mondadori in cui per la prima volta vengono editi con rigoroso metodo filologico e ampiamente commentati tutti i testi appartenenti a quella che viene unanimemente riconosciuta come la prima scuola poetica della nostra tradizione letteraria
Neutralizing antibody levels against SARS-CoV-2 variants of concern Delta and Omicron in vaccine breakthrough-infected blood donors
No full textBackground: Novel SARS-CoV-2 variants of concern (VOC) Delta and Omicron are able to escape some monoclonal antibody therapies, making again COVID-19 convalescent plasma (CCP) a potential frontline treatment. Study design/methods: In this study, we investigated the kinetics of anti-SARS-CoV-2 neutralizing antibodies (nAbs) against VOCs Delta and Omicron in vaccine breakthrough infected plasma donors. Serum samples from 19 donors were collected at the time of plasma donation and tested for anti-SARS-CoV-2 nAbs (using live authentic VOC viral neutralization test) and IgG (Liaison® SARS-CoV-2 S1/S2 and Liaison® SARS-CoV-2 TrimericS IgG assays, DiaSorin). Measures were correlated with different variables, including the time between last vaccine dose and CCP donation, and time between SARS-COV-2 infection and CCP donation. Results: nAb titers against VOC Delta and Omicron were directly related to the time interval since last vaccine dose to CCP donation, but inversely related to time since COVID19 breakthrough infection. Discussion: SARS-CoV-2 breakthrough infection in vaccinated in donors boosts nAb titers against VOCs Delta and Omicron, but such titers decay shortly after infection. Therefore, CCP must be collected early after vaccine breakthrough infection
Antibacterial activity and cytotoxic effect of SIAB-GV3
No full textThe aim of this study was to evaluate the antibacterial activity and cytotoxic effect of the compound SIAB-GV3, a new formulation presenting as an aqueous suspension of silicon dioxide (SiO2) functionalized with silver (Ag+) nanoparticles and benzalkonium. The product is formulated as an adjuvant for the treatment of inflammatory diseases of the oral cavity. This study demonstrates that SIAB-GV3 possesses strong antimicrobial activity against most of the common oral pathogens, in particular against Streptococcus pyogenes, Porphyromonas gingivalis and Aggregatibacter actinimycetemcomitans. The cytotoxic effect against human embryo lung fibroblasts (HELF cells) was evaluated at different times, from 1 h to 168 h, using concentrations of SIAB-GV3 ranging from 50 mg/ml to 0.0008 mg/ml. At the concentration of 10 mg/ml, commonly used in clinical practice, the compound results cyto- toxic after about 2 hours, this time being much longer than the typical time of local application, which is no more than 10 minutes. In conclusion, our findings suggest that SIAB-GV3 has a good antibacterial activity against the most common oral pathogens even at very low concentrations and a low cytotoxic activity, thanks to the synergistic effects of Ag nanoparticles, silicon dioxide and benzalkonium
Infezioni da Herpes Simplex (HSV) dopo trapianto cardiaco.
No full textLibro abstract del XXIII Congresso Nazionale Associazione Microbiologi Clinici Italiani, Montecatini Terme (PT) 5-8 ottobre 1994.(Poster
Viremic Dengue virus infections in travellers: Potential for local outbreak in Northern Italy
No full textDengue virus infection is a growing global problem and reports of outbreaks in Asia and Latin America as well as sporadic infections in international travellers are increasing. Two imported cases of serotype-1 Dengue virus infection in a Northern Italy region with high density of Aedes albopictus vector are described: a 27-year-oldman returning from Bali and a 25-years-oldwoman returning from Brazil. In both patients, viremia lasted for several days before disappearance. These cases stress the importance of investigating Dengue virus infections in febrile travellers, point to the potential for local outbreak of Dengue virus infection and emphasize the necessity of maintaining surveillance in non-endemic countries. (C) 2010 Elsevier B.V. All rights reserved
SARS-CoV-2 variants inactivation of plasma units using a riboflavin and ultraviolet light-based photochemical treatment
No full textBackground: Test the ability of Mirasol Pathogen Reduction Technology (PRT, Terumo BCT, Lakewood Co, USA) treatment with riboflavin and ultraviolet light (R + UV) in reducing SARS-CoV-2 infectivity while maintaining blood product quality. Material and methods: SARS-CoV-2 strains were isolated and titrated to prepare cell free virus for plasma units infection. The units were then under treatment with Mirasol PRT. The infectious titers were determined before and after treatment with an in house microtitration assay on Vero E6 cells. Thirty-six plasma pool bags underwent PRT treatment. Results: In all the experiments, the measured titer following riboflavin and UV treatment was below the limit of detection of microtitration assay for all the different SARS-CoV-2 strains. Despite the high copies number detected by RT-PCR for each viral strain after treatment, viruses were completely inactivated and not able to infect VERO E6 cells. Conclusion: Riboflavin and UV light treatment effectively reduced the virus titers of human plasma to the limit of detection in tissue culture, regardless of the strain. These data suggest that pathogen reduction in blood products highlight the safety of CP therapy procedures for critically ill COVID-19 patients, while maintaining blood product quality
A computational workflow for the design of irreversible inhibitors of protein kinases
No full textDesign of irreversible inhibitors is an emerging and relatively less explored strategy for the design of protein kinase inhibitors. In this paper, we present a computational workflow that was specifically conceived to assist such design. The workflow takes the form of a multi-step procedure that includes: the creation of a database of already known reversible inhibitors of protein kinases, the selection of the most promising scaffolds that bind one or more desired kinase templates, the modification of the scaffolds by introduction of chemically reactive groups (suitable cysteine traps) and the final evaluation of the reversible and irreversible protein-ligand complexes with molecular dynamics simulations and binding free energy predictions. Most of these steps were automated. In order to prove that this is viable, the workflow was tested on a database of known inhibitors of ERK2, a protein kinase possessing a cysteine in the ATP site. The modeled ERK2-ligand complexes and the values of the estimated binding free energies of the putative ligands provide useful indicators of their aptitude to bind reversibly and irreversibly to the protein kinase. Moreover, the computational data are used to rank the ligands according to their computed binding free energies and their ability to bind specific protein residues in the reversible and irreversible complexes, thereby providing a useful decision-making tool for each step of the design. In this work we present the overall procedure and the first proof of concept results
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