1,452 research outputs found

    Evaluación de la resistencia al herbicida Sethoxydim en accesiones de ischaemum rugosum salisb., Provenientes de arrozales venezolanos.

    No full text
    La paja rugosa (Ischaemum rugosum Salisb.), es la principal maleza de importancia de la producción de arroz paddy de Venezuela. Con el objetivo de evaluar la resistencia de accesiones de I. rugosum al herbicida sethoxydim,provenientes de los arrozales venezolanos, pertenecientes al banco de germoplasma del proyecto de manejo integrado para el manejo de malezas, (MIMA-UCV), se establecieron dos bioensayos, el primero de detección utilizando la dosis comercial de 112 g i.a L -1 en 21 accesiones y el segundo de respuesta a dosis en las accesiones IR90G (bispiribac-sodio, profoxidim, propanil, clomazone y fenoxaprop-etilo) e IR143G (susceptible a todos estos herbicidas) usando las dosis crecientes de sethoxidim 0, 14, 28, 56, 112, 224, 448 y 896 g i.a. ha -1 para IR90G y 0; 1,75; 3,5; 7;0; 14; 28, 56 y 112 g i.a. ha -1 para IR143G. El herbicida fue aplicado en ambos bioensayos cuando las plántulas tuvieron de 3 a 4 hojas. La descarga de la cámara de aplicación fue de 150 L ha -1 y se usó una boquilla Teejet 8002. A los 21 días después de la aplicación, se determinó la biomasa, expresándose en porcentaje de peso fresco sobre el testigo. Los resultados mostraron que de las 21 accesiones evaluadas en el bioensayo de detección una sola, procedente de Barinas, resultó ser susceptible a sethoxidim mientras que las otras fueron resistentes. La accesión IR143G, susceptible a otros herbicidas mencionados anteriormente, en este caso resultó ser resistente a sethoxidim igual que la IR90G, lo que constituye el primer caso de resistencia para esta accesión que es usada como accesión susceptible para todos los experimentos que se han conducido en el proyecto MIMA

    Food labelling: A brief analysis of European Regulation 1169/2011

    No full text
    The variety of labelling legislations of single European (EU) Member States hinders legal certainty for consumers. The standardisation pursued by EU Regulation 1169/2011 will at long last provide the 28 EU Member States with a common legislation. The present article carries out a summary benchmarking analysis of the new legislative framewor

    Altered expression of p53, but not Rb, is involved in canine prostatic carcinogenesis

    No full text
    Abnormalities in the retinoblastoma (Rb) and p53 tumour suppressor gene have been frequently detected in human and canine cancers, but never investigated in canine prostate cancer, considered a good model for the advanced and aggressive androgen-resistant prostate cancer in men. Therefore, the aim of this study was to evaluate the immunohistochemical expression of Rb and p53 in 6 normal canine prostates, 15 canine prostates with benign prostatic hyperplasia (BPH) and 10 prostatic carcinomas (PCs). In all normal samples, p53 was expressed in low number of epithelial cells, while a greater number of positive cells were observed in BPH and PC. The mean number of positive cells was statistically significantly higher in PCs than normal and hyperplastic prostates. A cytoplasmic or nucleo-cytoplasmic staining was observed in 5 out of 10 PCs. Rb protein was expressed in high number of normal, hyperplastic and neoplastic cells without a statistically significant differences. Considering that Rb is frequently lost in human prostate cancer, we suggest that Rb is not involved in canine prostatic carcinogenesis. On the other hand, the increased expression of p53 that corresponds to genetic defects in the p53 gene may be associated with the malignant growth of canine prostate cancer, conferring an apoptosis-resistant phenotype

    Evaluation Of Bone Regeneration Obtained With Autologous Or Heterologous-Deproteinized Bone

    No full text
    Objectives: The aim of this study was to investigate the different behaviour of autologous bone (AB) and heterologous-deproteinized bone (HDB) as grafting in pre-prosthetic bone regenerative procedures. AB was obtained from calvaria (ABc) and mandibular ramus (ABm), while HDB was swine commercial bone substitute. Methods: AB and HDB were used to fill bone defects in edentulous sites, in patients requiring implant insertion. Specimens from the regenerated sites were withdrawn at the moment of implant insertion, six months after grafting. Samples underwent light microscope observation. Then, immunohistochemical analyses were performed to assess expression of VEGF, procollagen I, bone sialoprotein (BSP), iNOS and Bax. Finally, TUNEL analysis was conducted to measure the main number of apoptotic cells in the different samples. Results: At light microscope observation, the investigated bone substitutes showed a good integration with the host tissue. Immunohistochemical analyses showed VEGF expression was higher in ABc than in ABm and HDB. In ABm samples, BSP was over-expressed while iNOS appeared down-expressed compared to ABc and HDB. Procollagen I and Bax resulted highly expressed in HDB compared to AB samples. TUNEL analysis showed high number of apoptotic cells in HDB samples. Conclusions: Clinically, all the grafts appeared integrated with the host tissue, indicating they can be successfully used in pre-prosthetic regenerative procedures. However, after six months, specimens from HDB grafting exhibit remaining resorption phenomena, demonstrated by the over-expression of Bax and by the presence of high quantity of procollagen I. Presence of diffuse apoptotic cells in HDB samples was confirmed by TUNEL analysis. In contrast, higher ossification, forwarding neoangiogenesis and lesser inflammation could be observed in AB graftin
    corecore