1,721,336 research outputs found
[1 lettre de C. Martinelli à Jules Massenet, 22 avril 1912] (manuscrit autographe)
No full textLui fait part de son désir de le rencontrer pour monter ses oeuvres avec "tout [s]on coeur et toute l'admiration pieuse que tout artiste doit avoir pour le Maître dont le cerveau a conçu tant de chefs-d'oeuvre". - Papier à en-tête : "Casino de Paramé. C. Martinelli. Directeur artistique
Il diritto di voto nell’esperienza costituzionale canadese. Un’analisi comparata
Full text linkL’oggetto del presente lavoro è costituito dal tema del diritto di voto nel contesto attuale dell’ordinamento costituzionale canadese, ordinamento centrale di questa analisi, esaminato in comparazione con le esperienze di alcuni ordinamenti di democrazia pluralista: Stati Uniti d’America, Australia, Nuova Zelanda e Italia. Nel condurre una analisi comparata tra ordinamenti collegati tra loro dalla comune appartenenza alla famiglia giuridica di common law, e quindi da un certo tipo di
tecnica di riconoscimento dei diritti, si ritiene necessario giustificare l’inserimento del caso italiano, in quanto costituisce l’ordinamento di provenienza e di riferimento dell’autore del presente elaborato, oltre ad essere rappresentativo della prospettiva della famiglia giuridica romanogermanica, il che consente di inquadrare metodologicamente il tema oggetto di questa disamina nell’ambito della comparazione tra common law e civil law. Oltre a ciò, la comparazione in una prospettiva diacronica e sincronica tra la normativa, la giurisprudenza e la dottrina presenti negli ordinamenti presi in esame sarà funzionale al fine di operare una ricostruzione il più possibile completa dei singoli profili legati al diritto di voto che si intendono trattare
“Trattamento riabilitativo integrato del disturbo specifico di apprendimento nel primo ciclo della scuola elementare”
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
SELECTION OF EPITOPE DIRECTED RECOMBINANT ANTIBODIES TO INHIBIT MUTATED NUCLEOPHOSMIN ACTIVITIES
Full text linkNucleophosmin (NPM1; also known as B23, NO38, or numatrin) is an abundant nuclear-cytoplasmic shuttling protein that is involved in different cellular processes such as centrosome duplication, cell cycle progression and stress response. NPM1 physically interacts with several nuclear proteins, including nucleolin, p120, p53 and Mdm2. Recently, it has been demonstrated that NPM1 forms a stable complex with the tumor suppressor p19/Arf and it is absolutely required for its correct localization and stabilization in the nucleolus. The observation of chromosomal translocations of the NPM1 gene in human hematopoietic cancers has suggested that NPM1 contributes to tumor development by activating the oncogenic potential of the fused protein partners. Moreover, it has been found that about one-third of primary adult Acute Myeloid Leukemia (AML) patients bear mutations in the last exon of the NPM1 gene, leading to an aberrant cytoplasmic localization of the protein (NPMc+). Interestingly, the accumulation of NPMc+ in the cytoplasm appears responsible for the delocalization of proteins that, under normal conditions, interact with wild type NPM1 in the nucleus. In particular, NPMc+ binds, delocalizes and inactivates the p19/Arf tumor suppressor, as well as the Fbw7γ F-box protein that is involved in the proteasome-dependent degradation of the c-Myc oncoprotein. These data prompted me to explore the possibility of blocking the aberrant activity of NPMc+ using NPMmutant-specific antibodies. I report here the isolation, from the ETH2-Gold phage display library, of a recombinant antibody in scFv format specific for the NPMc+ protein. It univocally targets the mutated region at the C-terminal end of NPMc+. The specificity of this antibody was evaluated in vitro by performing western immuno-blot analyses, immunofluorescence and immunoprecipitation assays. I show that the scFv antibody performs as well as the mouse monoclonal antibody specific for the same NPMc+ mutation and used as control. It is also able to immunoprecipitate the overexpressed and the endogenous NPMc+ proteins. Furthermore, I have found that the scFv can be efficiently expressed in mammalian cells as an intrabody, upon transfection of specific vectors that carry its cDNA sequence, and that it accumulates in the nucleus when fused to a nuclear localization signal (NLS). This evidence opened the opportunity to try the in vivo re-localization of NPMc+ from cytoplasm to the nucleus by using the recombinant antibody as a specific carrier. However, scFv-NLS fusions were unable to re-locate NPMc+ into the nucleus. The addition of multiple NLSs to the scFv did not modify the sub-cellular localization of NPMc+. In conclusion, I have been able to isolate a recombinant antibody that specifically targets NPMc+ protein. This antibody, directed against the C-terminal epitope of the mutated protein, has been biochemically and functionally characterized both in vitro and in vivo. The scFv successfully expresses as an intrabody in mammalian cells and specifically interacts with the native NPMc+ in vivo, thus giving the premises for its further development as a potential innovative therapeutic tool
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
“Proposta di trattamento riabilitativo del disturbo specifico di apprendimento nel primo ciclo della scuola elementare”
No full textAppropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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