771 research outputs found
PENGARUH SUPLEMENTASI STARBIO DALAM PAKAN DENGAN 40% DEDAK PADI TERHADAP PENAMPILAN BABI LANDRACE
No full textPenelitian bertujuan untuk mengetahui penampilan babi landrace yang diberi pakan mengandung 40% dedak padi dengan suplementasi Starbio. Penelitian ini menggunakan babi landrace sebanyak 12 ekor dengan berat badan antara 20-30 kg. Pakan terdiri dari konsentrat Guyofeed, dedak padi dan jagung kuning. Rancangan penelitian yang digunakan adalah rancangan acak kelompok (RAK); 4 kelompok berat badan yang diberi 3 macam pakan perlakuan. Pakan yang diberi dengan 40% dedak padi tanpa suplementasi Starbio sebagai kontrol (perlakuan A); pakan dengan 40% dedak padi dan disuplementasi 0,2% Starbio (perlakuan B); dan pakan dengan 40% dedak padi dan disuplementasi 0,4% Starbio (perlakuan C) selama 6 minggu. Data yang diperoleh dianalisis dengan analisis sidik ragam dan bila terdapat perbedaan yang nyata diantara perlakuan (P<0,05) dilanjutkan dengan uji jarak berganda Duncan (Steel dan Torrie, 1989). Dari hasil penelitian dapat disimpulkan bahwa suplementasi Starbio dalam pakan dengan 40% dedak padi mampu memperbaiki penampilan (konsumsi pakan, pertambahan berat badan, energi tercerna, dan profil kotoran) babi landrace.</jats:p
PENGARUH SUPLEMENTASI STARBIO DALAM PAKAN DENGAN 40% DEDAK PADI TERHADAP PENAMPILAN BABI LANDRACE
No full textPenelitian bertujuan untuk mengetahui penampilan babi landrace yang diberi pakan mengandung 40% dedak padi dengan suplementasi Starbio. Penelitian ini menggunakan babi landrace sebanyak 12 ekor dengan berat badan antara 20-30 kg. Pakan terdiri dari konsentrat Guyofeed, dedak padi dan jagung kuning. Rancangan penelitian yang digunakan adalah rancangan acak kelompok (RAK); 4 kelompok berat badan yang diberi 3 macam pakan perlakuan. Pakan yang diberi dengan 40% dedak padi tanpa suplementasi Starbio sebagai kontrol (perlakuan A); pakan dengan 40% dedak padi dan disuplementasi 0,2% Starbio (perlakuan B); dan pakan dengan 40% dedak padi dan disuplementasi 0,4% Starbio (perlakuan C) selama 6 minggu. Data yang diperoleh dianalisis dengan analisis sidik ragam dan bila terdapat perbedaan yang nyata diantara perlakuan (P<0,05) dilanjutkan dengan uji jarak berganda Duncan (Steel dan Torrie, 1989). Dari hasil penelitian dapat disimpulkan bahwa suplementasi Starbio dalam pakan dengan 40% dedak padi mampu memperbaiki penampilan (konsumsi pakan, pertambahan berat badan, energi tercerna, dan profil kotoran) babi landrace
Assessing the effects of spatial and temporal variation in sediment conditioning on seagrass seed germination and seedling performance
No full textMason J. Tsaglos1, Forest R. Schenck2, and Torrance C. Hanley1
1Sacred Heart University, Department of Biology, Fairfield, CT
2Massachusetts Division of Marine Fisheries, Salem, MA
Seagrass meadows provide numerous ecosystem functions and services, making conservation and restoration of these ecologically-valuable systems a high priority for coastal managers. The global decline of seagrass meadows necessitates finding consistently effective restoration methods. Recently, there has been increased interest in seed-based methods of seagrass restoration. However, these methods have had mixed success, with highly variable outcomes. Relatively little is known about how sediment characteristics, including soil conditioning by seagrasses at different spatial and temporal scales, may affect seed germination and seedling success in a restoration context. To examine the effects of seed source and sediment characteristics, we conducted a laboratory experiment using eelgrass seeds collected from different source meadows and comparing i) sediment collected different distances from a natural meadow (0, 1, 25, and 250 m from the edge) and ii) sediment that varied in historical seagrass presence (seagrass present currently and seagrass present ~1, 5, 10, and 20 years ago). We measured seed germination and seedling performance, and also assessed seed quality and viability. Our results can be used to inform seagrass restoration in New England, suggesting that i) sourcing seeds from multiple meadows will increase the likelihood of including higher quality seeds, and ii) including sediment inoculations from vegetated sites may increase success
Age-Related Deficits in Electron Transport Chain Complexes in Rat Neurons and 3xTg-AD Mouse Neurons
No full textIn neurons, mitochondrial quantity and basal cellular respiration are maintained with age, but alterations in other key functions and quantities make these cells susceptible to the pathology of age-related neurodegenerative disease. We observed age-related decreases in cytochrome C, cardiolipin, cytochrome C oxidase (CCO) function, and glutamate response that render cells less capable of responding to stress. Rescue experiments showed that estrogen is a promising treatment in restoring neuron function with age. After finding key differences in CCO, we examined the electron transport chain more closely and found age-related deficits in quantity or function for each individual complex. Our experiments support a lack of endogenous substrates or a failure of upstream complexes to transport electrons to complex IV with age, ultimately leading to age-related neurodegeneration. Reactive oxygen species production may add to the problem by degrading macromolecules such as nucleic acid, cardiolipin, and proteins. Increased ROS may also lead to a redox imbalance in the neuron, reducing the potential for energy production. Also, epigenetic controls such as DNA methylation, histone acetylation ubiquitination and phosphorylation that persist in culture independent of aging hormone levels, vasculature, and immune system may be partly responsible for the observed age-related deficiencies as has been previously observed in aging human muscle (Ronn et al., 2008). This compelling cumulative evidence suggests an age-related deficiency in electron transport via quinones from complexes I to III, and age-related deficiencies in substrates, cofactors, and quantity or function for complex IV. These studies add to the growing body of evidence that dysfunction in the enzyme complexes of the electron transport chain lead to neurodegeneration in senescence-related diseases. In an attempt to integrate our age-related findings with Alzheimer\u27s Disease (AD) pathology, we sequentially isolated the electron transport chain complexes using selective mitochondrial inhibitors in cortical neurons removed from the 3xTg-AD mouse model, which harbors mutations in the PS1, APPSwe and tauP301L genes and follows the proposed temporal development of human AD pathology (Oddo et al., 2003a; 2003b). Overall, we did not detect 3xTg-AD cortical neuron deficits at the four electron transport complexes of mitochondria or in NAD(P)H oxidase (NOX), an extramitochondrial oxygen consumer and regulator of NAD(P)+/NAD(P)H homeostasis (Morre et al., 2000)
Neutron powder-profile study of chlorofluormethane
No full textThe crystal structure of chlorofluoromethane, CH\u2082C\u2081F, has been determined at 100 and 30 K using indexing, packing considerations and Rietveld refinement of neutron powder profiles. There is only one phase, in monoclinic space group P2\u2081 and with two molecules in the unit cell occupying general positions. The structure has close packing in several directions and there are weak hydrogen bonds forming zigzag chains.NRC publication: Ye
In the shadow of Disneyland and conservative Orange County politics: Latinx resistance in Anaheim and Santa Ana, 1942-2012
No full textFrom 1942 to 2012, Latinx activists from Orange County, California repeatedly mobilized against the threat of displacement and criminalization. Rapid suburban development and the struggle to control (and contain) Latinx labor coincided with the rise of mass incarceration and mass expulsions. Law enforcement, immigration authorities, and local leaders tried to thwart the growing Latinx populations in ways that made their labor readily available, while limiting their ability to build community in desirable areas marked for development. By focusing on the two cities with the largest Latinx and Black populations in Orange County, I trace how Santa Ana emerged as a hotbed of radical activism, while Anaheim’s Latinx residents combated displacement from the Disney corporation and Republican establishment. Through the examination of oral histories, archival documents, and newspaper articles I have unearthed a rich oppositional political culture that directly challenged the conservative governance of Orange County. In order to reframe the political history of Orange County from the perspective of Latinx and Black residents, I utilized over sixty oral histories from the Lawrence de Graaf Center at California State University, Fullerton. In addition, I have also conducted nine oral histories myself which will be deposited at the De Graaf Center which was funded by the John Whiteclay Chambers Oral History Fellowship at Rutgers University. The activism coming out of Anaheim and Santa Ana in the postwar period is extremely important for understanding Latinx community formation in the context of a conservative political climate. Shifting the gaze away from white residents’ grassroots mobilization to their effects on Latinx residents, my dissertation seeks to excavate long-term survival strategies against settler-colonialism in the Southwest borderlands of Orange County. By providing a grassroots urban history of the two largest Latinx communities in Orange County, this dissertation reconceptualizes the racial geography of the region. It disrupts the dominant historiography on suburbanization which too often eclipses Latinx residents’ contributions to Orange County’s political culture. In addition, it builds on the growing mass incarceration historiography by exploring the role that Latinx political leaders and large corporations played in displacing and criminalizing Latinx and Black communities. Finally, “In the Shadow of Orange County” expands upon Latinx historiography by analyzing the complicated inter- and intra-ethnic politics of grassroots organizing in which demographic transformation led a segment of the population to assume positions of power, while consigning others to new forms of criminalization and control. Today, with the expansion of the radical right, xenophobia, white supremacy, housing crises, and mass incarceration, Latinx and Black activism in conservative Orange County provides important historical insight into how vulnerable populations have organized against these forces in the past.Ph.D.Includes bibliographical reference
IN VITRO FERMENTABILITY,DEGRADABILITY AND MICROBIAL BIOMASS PRODUCT OF COMPLETE RATION CONTAINING A COMBINATION OF FIELD GRASS,CONCENTRATE AND NUTRIENT RICH SUPPLEMENT
No full textThe objective of thi experiment wa to obtain an optimum combination between field gra ,
concentrate and Nutrient Rich Supplement (NRS)ba ed on in vitro tudy using Hohenheim ga te t.The
experimental diet were:R1 (control diet =70%field gra +30%concentrate),R2 (70%field gra +
25%concentrate +5%NRS),R3 (70%field gra +20%concentrate +10%NRS)and R4 (70%field
gra +15%concentrate +15%NRS).A randomized block de ign with four treatment and four
replications wa carried out.Buffalo rumen fluid wa taken in different time and wa used a block or
replication.Data were analyzed by Analysi of Variance (ANOVA).Significant difference among
treatment were determined by contra t orthogonal.The re ult howed that total ga production and
total VFA concentration were highe t (P<0.05)in R4 among the treatment .Addition of 15%NRS in
complete ration (R4)increa ed NH3 concentration 10.34%.Addition of 10%NRS and 15%NRS in
complete ration (R3 and R4)improved the microbial bioma product compared to control and 5%NRS
in complete ration (R1 and R2).Dry matter (DM)and organic matter (OM)degradability were
ignificantly higher (P<0.01)in 10%NRS and 5%NRS in complete ration (R3 and R2)compared to
control and 15%NRS in complete ration (R1 and R4).It wa concluded that combination between 70%
field gra ,20%concentrate and 10%NRS in complete ration (R3)wa more optimal for improving ga
production,total VFA,NH3 concentration,microbial bioma product,dry matter degradability and
organic matter degradability,compared to control ration.
Keywords :omplete ration,NRS (Nutrient Ri h Supplement),fermentability,degradability,
mi robial biomass produc
Identification of inhibitors of the <i>Leishmania</i> cdc2-related protein kinase CRK3
No full textNew drugs are urgently needed for the treatment of tropical parasitic diseases such as leishmaniasis and human African trypanosomiasis (HAT). This work involved a high-throughput screen of a focussed kinase set of similar to 3400 compounds to identify potent and parasite-selective inhibitors of an enzymatic <i>Leishmania</i> CRK3-cyclin 6 complex. The aim of this study is to provide chemical validation that <i>Leishmania</i> CRK3-CYC6 is a drug target. Eight hit series were identified, of which four were followed up. The optimisation of these series using classical SAR studies afforded low-nanomolar CRK3 inhibitors with significant selectivity over the closely related human cyclin dependent kinase CDK2
Stable prenucleation mineral clusters are liquid-like ionic polymers
No full textCalcium carbonate is an abundant substance that can be created in several mineral forms by the reaction of dissolved carbon dioxide in water with calcium ions. Through biomineralization, organisms can harness and control this process to form various functional materials that can act as anything from shells through to lenses. The early stages of calcium carbonate formation have recently attracted attention as stable prenucleation clusters have been observed, contrary to classical models. Here we show, using computer simulations combined with the analysis of experimental data, that these mineral clusters are made of an ionic polymer, composed of alternating calcium and carbonate ions, with a dynamic topology consisting of chains, branches and rings. The existence of a disordered, flexible and strongly hydrated precursor provides a basis for explaining the formation of other liquid-like amorphous states of calcium carbonate, in addition to the non-classical behaviour during growth of amorphous calcium carbonate
Breakdown of the modified Gouy-Chapman model for clay surfaces in equilibrium witha CaCl 2 electrolyte: a molecular dynamic study
No full textInternational audienceClay minerals have a high specific surface area that enhances their ability to influence water in their vicinity. Their surface charge, which varies as a function of physical-chemical conditions of the surrounding water, results in a complex organization of solute ions as a function of distance from the surface. This organization is known as the electrical double layer where counter-ions are electrostatically attracted to the surface whereas co-ions are repelled from it. Abundant and convincing spectroscopic evidence has emerged of a complex layering of water molecules and ionic species adsorbed at specific distances from the charged surfaces, a feature that can be captured by surface complexation models derived from Stern theory. The modified Gouy-Chapman model (MGC), or its modified version based on the resolution of the full Poisson-Boltzmann equation, is often used to calculate the electrostatic potential value in the diffuse region between the Stern layer and the bulk solution. Based on a comparison of the MGC model with Monte-Carlo simulations of discrete hard-sphere ions immersed in an implicit solvent, it was demonstrated long ago that the resolution of the full Poisson-Boltzmann equations can give accurate results for cations and anions distribution in the vicinity of a charged surface if both anions and cations are monovalent, if their total concentration does not exceed 0.1 mol/dm3 and if the surface charge is not too high. When divalent (or multivalent) ionic species are present, the MGC model fails at reproducing Monte-Carlo simulations results in most conditions, because the MGC model neglects ion-ion interactions. For example, for 2:1 electrolytes the MGC model is thought to be inaccurate at salt concentrations greater than 0.005 mol/dm3 (Torrie and Valleau, 1982; Carnie and Torrie, 1984; Boda et al., 2004).Deviations from Poisson-Boltzmann model predictions probed by spectroscopic tools are now also available. Bu et al. (2006) demonstrated, using anomalous X-ray reflectivity data, that the MGC model is strikingly accurate at a highly charged interface in contact with a homovalent monovalent salt background up to a concentration of 10-2 mol dm-3. Another study performed in similar conditions found that while accurate at 0.01 mol dm-3, the Poisson-Boltzmann model failed to reproduce X-ray reflectivity data at higher concentrations (Luo et al., 2006). Molecular dynamics (MD) calculation studies have provided broadly similar results to those obtained by X-ray reflectivity (Wang and Chen, 2007; Lima et al., 2008; Lee et al., 2012). However, these studies were performed using homovalent monovalent electrolytes that are not generally representative of environments that are rich in clay minerals, whose surface sites tend to be occupied by divalent cations in natural clay formations. In the present study, we evaluated the accuracy of MGC model predictions in a clay/water system in the presence of divalent cations and monovalent anions using MD calculations. Clay surfaces present several advantages with regards to molecular simulations owing to the 2D pseudo-periodicity of the clay particles. This system is particularly well suited to enabling accurate comparison between the MGC model prediction and the actual distribution of ions at the solid-water interface. Amongst clay minerals, montmorillonite has been the matter of multiple of MD simulation studies, but the vast majority of them involved only monovalent cations in interlayer spaces without the presence of anions. Few studies have been focused on divalent cations and/or charged surfaces in contact with large pores where the diffuse layer can fully extend without overlapping (Tournassat et al., 2009; Bourg and Sposito, 2011). We will show that the MGC model prediction of the electrostatic potential value at the surface as well as the charge compensation over the distance from the surface are considerably more accurate than previously inferred from its comparison with Monte-Carlo simulations of discrete hard-sphere ions immersed in an implicit solvent
- …
