1,720,996 research outputs found
Expression of flgK flagellin from Salmonella Typhimurium in tobacco seeds
No full textIn the epidemiology of Salmonella Typhimurium, recognized as major zoonotic pathogens of animals and humans, swine plays an reservoir role. Novel methods are requires to control salmonellosis in livestock, and vaccination represent an efficacious way to prevent the disease, reducing antibiotic treatments, as suggested by EC Regulation 1831/2003. In this contest plants have considerable potential and represent a promising alternative for biopharmaceutical protein (plant-vaccine). For these reasons the aim of this study was to engineer tobacco plants in order to induce the seed specific expression with correct folding of flgK protein from Salmonella typhimurium for the subsequent utilization as edible vaccine or as an advantageous system of production. flgK gene was isolated by a polymerase chain reaction (PCR) from a wild type Salmonella typhimurium strain, and transferred into a expression cassettes under control of seed-specific GLOB promoter in order to induce, after agroinfection, the expression of flgK in seeds. Transformed plants showed a correct transcription of flgK gene and the estimated amount of expressed flgk was about 0.6 mg per gram of seeds corresponding to 0.3% of the total soluble protein. In conclusion our data showed flgK flagellin could be expressed in tobacco seeds and stably incorporated into plant genoma
Storage-time effects on olive oil DNA assessed by Amplified Fragments Length Polymorphisms
No full textIn this study Amplified Fragments Length Polymorphisms (AFLPs) analysis was applied on DNA extracted from different monovarietal olive oils. The aim was to study how the length of storage after milling of the oil can affect the use of DNA as an analyte for molecular traceability. Results, all assessed by statistical analyses, showed that the authentication of olive oil with molecular methods should be performed within a month from olive oil production. After this period, a significant decrease of quality of DNA extracted from olive oil was observed, with a consequent loss of information, that can affect the reliability of the results
Evaluation of gastric degradability of antigenic protein espresse in tobacco seeds
Full text linkPlants have been recognized as expression system for the production of edible vaccine because of the possibility of introducing antigenic proteins into their genoma. In livestock, transformed plants for the expression of immunogenic proteins, could be administered, orally, in feed to induce mucosal immune response in the gastrointestinal tract. Moreover edible vaccines for veterinary use could reduce costs of traditional vaccines associated with the production, the cold storage, and parenteral administration. However the most important problem related to the oral delivery route is the potential for antigen degradation in the gastrointestinal tract. For these reasons the aim of this study was the evaluation of the effect of swine gastric fluid on VT2e-B antigenic protein, derived from a strain of Oedema disease E. coli, expressed in tobacco seeds by Agroinfection. Samples of transgenic tobacco seeds, both milled and whole, were incubated with porcine gastric fluid, at 38° C in Dubnoff Shaker for 1, 2 and 3 hours. After gastric fluid removal, by centrifugation and washing with PBS, samples were homogenized in the presence of protein extraction buffer. Western blot was performed on representative samples of extracted proteins, quantified by Bradford method, using rabbit polyclonal serum. The Vt2e-B specific signal was observed in all samples derived from transgenic tobacco seeds. Nevertheless, from 0 h to 3 h, a progressive reduction of intensity of signal was observed. No significant differences were detected on the reduction of signal intensity between samples derived from whole and milled tobacco seeds
Antigenic recombinant proteins expressed in tobacco seeds as a model for edible vaccines against swine oedema
No full textOedema can cause morbidity and mortality in piglets and is a source of economic loss
in the pig industry world-wide. Plant based oral vaccines offer a new approach to
vaccination strategies especially in the prevention of infection by enteric pathogens.
Transgenic plant seeds are extremely well suited for the targeted synthesis of heterologous
proteins and offer many advantages over conventional expression systems for
the production of recombinant subunit vaccines
Vecchie varietà di melo (Malus domestica Borkh.) individuate nel Casentino (Arezzo-Toscana): caratterizzazione morfologica, biometrica e melecolare (SSR).
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