1,721,030 research outputs found
Tecniche per l’identificazione dei valori soglia nei test di screening : il caso dell’ipotiroidismo congenito primario
No full textVariability on Catellatospora spp. strains isolated from plant materials
No full textNumerous strains of actinomycetes belonging to the "rare" genera were isolated from different plant materials as leaf litters and internal root cortical tissues; among them 53 were identified as members of the genus Catellatospora on the basis of the micromorphology of the reproductive structures. The variability characterizing these strains was studied, in comparison to 5 type strains of the genus, evaluating the cultural features on agar media and the ARDRA profiles obtained by three endonucleases Cfol, HaeIII and HpaII. The different profiles highlight the wide phenotypic and genotypic variability of these strains and the remarkable differences among most of them in comparison to the described ones. Only few strains showed characteristics similar to a type strain, in particular to Catellatospora citrea subsp. citrea, whereas several of them could belong to new taxa. The results achieved were also particularly remarkable under the ecological point of view considering the high number of studied wild isolates, in comparison to the knowledge on these genus based mostly on the descriptions of the few type strains. Furthermore the examined population was strictly connected with plant materials, in particular with leaf litter, instead the type strains were all isolated from woodland soil
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Diagnosis of congenital cytomegalovirus infection by detection of viral DNA in dried blood spots
No full textBackground: The reference method of cytomegalovirus (CMV) isolation from urine or saliva is not a feasible routine technique for all newborns,- and laboratory -diagnosis -of- this- infection-would be useful- -both for--epidemiological purposes and to enable prompt institution of adequate measures to identify and correct late sequelae. Extraction and amplification of viral DNA from dried blood spots (DBS) collected from babies in the first days of life during routine screening for genetic and metabolic disorders has been proposed for the early diagnosis of viral congenital infections.
Objectives: To test the method for CMV DNA extraction from DBS and to evaluate the results obtained in newborns with and without a diagnosis of congenital infection based on viral isolation from urine and or saliva at birth.
Study design: DBS from Guthrie cards collected in babies who underwent virological tests for CMV infection were tested for CMV DNA by observers blinded to the virological results. DNA was extracted from DBS both in water and in cell culture medium according to Shibata et al. with minor modifications. The products of nested polymerase chain reactions (PCR) amplifying two regions in the 1E1 and gp58 genes were detected by agarose gel electrophoresis. Strict control measures were adopted to avoid carryovers and contaminations.
Results: DBS from the eight symptomatic and 11 asymptomatic congenitally infected babies were positive when extraction was performed in medium, whereas extraction in water failed to identify two of the asymptomatic cases. The results obtained with the two extraction methods agreed in the remaining cases; the 71 CMV negative control babies were negative and two out of 21 cases of supposed postnatal infection were diagnosed as congenital on the basis of a positive DBS. All positive cases were identified by gp58 PCR but only slightly over half of them by 1E1 PCR. Extraction in medium was more efficient than in water.
Conclusions: The method of CMV DNA extraction in medium followed by amplification of the gp58 region showed 100% sensitivity and specificity compared with isolation in cell culture. Therefore, we propose this procedure to diagnose congenital CMV infection at birth and also later
Palmoplantar keratoderma of Unna-Thost : response to biotin in one family
No full textThree members of a family with Unna-Thost palmoplantar keratoderma who showed skin improvement whilst receiving oral biotin are described
Dried newbom blood spots examination allows congenita) CMV infection diagnosis
No full textAn accurate, easy and widely available method of diagnosing congenitally CMV infection as an alternative to the laborious virus isolation in celi culture is important for clinica) and epidemiological purposes. We report on a method for CMV DNA search in dried newbom spots (DBS) collected on Guthrie cards for the screening for metabolic and genetic disorders. The procedure was tested on 285 babies boro in our region between 1994 and 1996 who underwent virus isolation testing in the first two weeks of life to assess congenita] CMV infection. Blood was eluted from filter paper in MEM and heat extracted. The products of a nested PCR amplifying a region of 100 bp in the gp 58 gene were detected by agarose gel electrophoresis. Strict control measures were adopted to avoid carryovers and contaminations. The test was positive in ali congenitally infected babies, both symptomatic at birth (n=10) and asymptomatic (n=21), and in six out of 254 children CMV isolation negative. Positive DBS were collected between 3 days and 22 months before examination and have been stored in standard conditions. We propose this method as a valid procedure to diagnose congenita! CMV infection. Due to the ease of collection, storage and shipment of DBS few laboratories performing the test properly could allow the diagnosis congenita] CMV infection to be widely available
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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