797 research outputs found

    Effects on the feline fibrosarcoma Cocca-6A cells following Adenoviral gene transfer.

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    <p>(A) Phase contrast imaging of Ad.CMV transduced Cocca-6A control cells at 24 hours (200× magnification). (B) Fluorescence imaging (200× magnification) of the Ad.GFP-transduced Cocca-6A control cells at 24 hours. (C) Flow-cytometric diagram of Ad.GFP transduction of Cocca-6A cells (50 MOI, 24-hours after transduction). (C–H) Phase contrast imaging (200× magnification) of Ad.Rb/p105, Ad.Ras-DN, Ad.mda7/IL-24, CTV/IFNγ, and CTV-mda7/IL-24 transduced Cocca-6A cells (50 MOI, 24-hours after transduction).</p

    Karyogram of the feline fibrosarcoma Cocca-6A cells.

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    <p>(A) Oil immersion microphotograph (1000× magnification) of Giemsa block stained spotted metaphases from a representative colchicine treatment of the Cocca-6A cells. The Cocca-6A cells show the absence of one of the E1 chromosomes. (B) Semi-quantitative PCR of p53 gene in human HEK-293, feline FSkMC and Cocca 6A cells.</p

    The Effects of the combination of Adenoviral gene transfer on the feline fibrosarcoma cells Cocca-6A.

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    <p>Every panel shows the phase contrast imaging (200× magnification, 24-hours post transduction) and the propidium iodide flow cytometry analysis of Cocca-6A cells transduced with 50 MOIs of various combinations of double or triple transductions using Ads carrying Ad.<i>RB/p105</i>, Ad.<i>Ras</i>-DN, Ad.<i>mda-7/IL24</i>, CTV.<i>mda-7/IL24</i> or CTV.<i>IFNγ.</i> (a–g) Single adenoviral transduction and controls: (a) Control untransduced Cocca-6A cells. (b) Ad.GFP, (c) Ad.Rb/p105, (d) Ad.Ras-DN, (e) Ad.Mda7/IL-24, (f) CTV.Mda7/IL-24, (g) CTV.IFNγ transduced Cocca-6A cells. (h–q) Combinations of double or triple adenoviral transductions: (h) Ad.Rb/p105+ Ad.Ras-DN, (i) Ad.Rb/p105+ Ad.Mda7/IL-24, (j) Ad.Rb/p105+ CTV.Mda7/IL-24, (k) Ad.Rb/p105+ CTV.<i>IFNγ,</i> (l) Ad.Ras-DN + Ad.Mda7/IL-24, (m) Ad.Ras-DN + CTV.Mda7/IL-24, (n) Ad.Ras-DN + CTV.<i>IFNγ,</i> (o) Ad.Rb/p105+ Ad.Ras-DN + Ad.mda7/IL-24, <i>(p)</i> Ad.Rb/p105+ Ad.Ras-DN + CTV.mda7/IL-24, (q) Ad.Rb/p105+ Ad.Ras-DN + Ad.CTV.<i>IFNγ</i> transduced Cocca-6A cells.</p

    Comics and human rights: the erasure of X-Women in days of future past

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    Carolyn Cocca is Associate Professor in the Department of Politics, Economics, and Law at the State University of New York, College at Old Westbury. She is the author of Jailbait: The Politics of Statutory Rape Laws in the United States (SUNY Press), and most recently, of “The Brokeback Project: A Quantitative and Qualitative Analysis of Portrayals of Women in Mainstream Superhero Comics, 1993-2013”, “Negotiating the Third Wave of Feminism in Wonder Woman” and “Re-booting Barbara Gordon: Batgirl, Oracle, and Feminist Disability Theories” in ImageTexT. She teaches courses on U.S. politics, civil liberties and civil rights law, and the politics of gender and sexuality

    Comics and human rights: Wonder Woman and the trickiness of superheroines

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    Carolyn Cocca is Associate Professor in the Department of Politics, Economics, and Law at the State University of New York, College at Old Westbury. She is the author of Jailbait: The Politics of Statutory Rape Laws in the United States (SUNY Press), and most recently, of “The Brokeback Project: A Quantitative and Qualitative Analysis of Portrayals of Women in Mainstream Superhero Comics, 1993-2013”, “Negotiating the Third Wave of Feminism in Wonder Woman” and “Re-booting Barbara Gordon: Batgirl, Oracle, and Feminist Disability Theories” in ImageTexT. She teaches courses on U.S. politics, civil liberties and civil rights law, and the politics of gender and sexuality

    Comics and human rights: oracle and representations of disability in superhero comics

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    Carolyn Cocca is Associate Professor in the Department of Politics, Economics, and Law at the State University of New York, College at Old Westbury. She is the author of Jailbait: The Politics of Statutory Rape Laws in the United States (SUNY Press), and most recently, of “The Brokeback Project: A Quantitative and Qualitative Analysis of Portrayals of Women in Mainstream Superhero Comics, 1993-2013”, “Negotiating the Third Wave of Feminism in Wonder Woman” and “Re-booting Barbara Gordon: Batgirl, Oracle, and Feminist Disability Theories” in ImageTexT. She teaches courses on U.S. politics, civil liberties and civil rights law, and the politics of gender and sexuality

    Western blot analysis of Cocca-6A cell lysates following adenoviral transduction.

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    <p>On the left lane are loaded control Cocca-6A cells. In the lane on right are loaded the transduced Cocca-6A cells. Anti beta-actin was used as a loading control. 50 µg of total lysates were run in SDS polyacrylamide gels. On the left side are indicated the different adenoviral transductions.</p

    Lack of effects on the feline fibrosarcoma Cocca-6A cells following Adenoviral gene transfer.

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    <p>(A–G) Phase contrast imaging (200× magnification) of Ad.p53, Ad.p130, Ad.p18, Ad.p19, Ad.p21, Ad.p27, and Ad.pTEN transduced Cocca-6A cells (50 MOI, 24-hours after transduction).</p
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