35 research outputs found
A bioprocess optimization study to enhance the production of Menaquinone-7 using Bacillus subtilis MM26
No full textAbstract Background Menaquinone-7 (MK-7) has a vital significance in promoting human health and tackling several global health concerns which makes its production extremely important. MK-7 is not easily accessible at a reasonable cost due to the poor fermentation yields and the existence of several laborious downstream unit processes. Efficient manufacturing methods are essential to meet the global requirements due to the increasing demand in the pharmaceutical and nutraceutical industries. This research study focuses on the enhanced production of MK-7 from Bacillus subtilis MM26 isolated from fermented home-made wine. Results A suitable MK-7 production medium for Bacillus subtilis MM26 was determined and the yield was found to be 67 ± 0.6 mg/L. The one factor at a time (OFAT) results showed that medium containing lactose, glycine, with a pH 7, a temperature of 37 °C, and an inoculum size of 2.5% (2 × 10⁶ CFU/mL) was optimal synthesis of MK-7. RSM indicated that incubation time, carbon and nitrogen sources were the factors significantly affecting the MK-7 yield. RSM predicted optimal conditions, which yielded a maximum concentration of 442 ± 2.08 mg/L of MK-7. Conclusions The outcomes of this study demonstrated the potential of Bacillus subtilis MM26 in large-scale industrial production of MK-7. The yield of MK-7 was amplified efficiently by integration of OFAT and RSM, paving the way for cost-efficient industrial production
Potential application of immobilized streptokinase extracted from <i>Streptococcus equinus</i> VIT_VB2
No full textAcid stable α-amylase from Pseudomonas balearica VITPS19—Production, purification and characterization
No full textIn the present study, α-amylase from Pseudomonas balearica VITPS19 isolated from Kolathur, Tamil Nadu, India was studied. Initially, one factor at a time (OFAT) approach was used to optimize the medium parameters like pH, temperature, carbon and nitrogen sources and the presence of metal ions to enhance the amylase activity. After the optimization, 6.5-fold increase in the enzyme production was observed. Enzyme purification was carried out in three stages. The molecular weight of purified α-amylase was estimated to be 47 kDa.The optimum activity for the purified enzyme was observed at pH 6 in 0.1 M phosphate buffer at 25 ± 2 °C and the activity is enhanced in the presence of ions like Mn2+, Mo6+, Na+, Mg2+and Zn2+ and was inhibited in the presence of Hg2+ ions. Compounds such as Sodium dodecyl sulfate (SDS), Ethylenediaminetetraacetic acid (EDTA), urea and β- mercaptoethanol reduced the amylase activity. The Km and Vmax of the α-amylase was estimated to be 45.23 mM and 20.83 U/mL, respectively
Bio prospecting of Riboflavin producing bacteria from different riboflavin enriched food sources
No full textRiboflavin is an essential, water-soluble vitamin (B2) and a component of basic cellular metabolism. The aim of the present study is to isolate and characterize riboflavin producing bacteria from different food sources. Ten different riboflavin enriched food sources were collected from Vellore district. Totally 72 bacterial strains were isolated and cultured on nutrient agar plates. Out of these, 43 strains were identified as riboflavin producers. Isolated bacterial strains HDS27, HDS07, HDS14, HDS18, HDS38 and HDS54 isolated from milk, mushroom, spinach, lamb kidney, beef liver and mackerel fish were found to be potent riboflavin producers. Based on morphological, biochemical and molecular characterization, the potent strains were identified as Lactobacillus plantarum (HDS27), Bacillus cereus (HDS07), Delftia tsuruhatensis (HDS14), Citrobacter freundii (HDS18), Enterobacter cloacae (HDS38) and Bacillus cereus (HDS54). The selected potent isolates HDS27 from milk and HDS07 from mushroom showed a maximum riboflavin production of 3.69 mg/L and 2.9mg/L respectively. The present study explores the riboflavin producing novel bacteria from different food sources. This is the first report that the Enterobacter cloacae isolated from beef liver, Delftia tsuruhatensis from spinach and Citrobacter freundii from lamb kidney has the ability to produce riboflavin. These potent strains could be a better starter for substituting the conventional bacteria for large scale production of riboflavin in industry
Biosynthesis and therapeutic applications of MK-7: A comprehensive review
No full textThe fat-soluble vitamin K is an indispensable cofactor that transmutes the glutamic acid residues to -γ-carboxyglutamic residues in blood and bones. Vitamin K is further classified into three namely, Phylloquinone, Menaquinone and Menadione. Both, phylloquinone and menaquinone are naturally derived types of vitamin K while Menadione is a synthetic variant. Among the several types of vitamin K, Menaquinone-7 (MK-7) stands out because of its extended half-life. MK-7 is found to have potential therapeutic effects in preventing cardiovascular disease, osteoporosis, diabetes, Alzheimer and cancer. Comprehending the diverse functions of MK-7 provides valuable perspectives on its ability to enhance overall health. During microbial fermentation, certain strains are selected and improved for effective synthesis of MK-7. Researchers are investigating industrial-scale production techniques such as fermentation conditions, downstream processing, and purification methods to increase both quantity and quality. This review highlights MK-7’s diverse biological functions and industrial significance, emphasizing advancements in microbial fermentation, including strain improvement and production optimization, to enhance yield and quality
Properties of Concrete Manufactured Using Steel Slag
No full textAbstractThis paper aims to study experimentally, the effect of partial replacement of coarse and fine aggregates by steel slag (SS), on the various strength and durability properties of concrete, by using the mix design of M20 grade. The optimum percentage of replacement of fine and coarse aggregate by steel slag is found. Workability of concrete gradually decreases, as the percentage of replacement increases, which is found using slump test. Compressive strength, tensile strength, flexural strength and durability tests such as acid resistance, using Hcl, H2SO4, and Rapid chloride penetration, are experimentally investigated. The results indicate that for conventional concrete, the partial replacement of fine and coarse aggregates by steel slag improves the compressive, tensile and flexural strength. The mass loss in cubes after immersion in acids is found to be very low. Deflection in the RCC beams gradually increases, as the load on the beam increases, for both the replacements. The degree of chloride ion penetrability is assessed based on the limits, given in ASTM C 1202. The viability of usage of SS in concrete is found
In vitro thrombolytic potential of bioactive compounds from marine Streptomyces sp. VITJS4
No full textThe most practical approach to reduce morbidity and mortality of coronary heart disease (CHD) is to delay the process of thrombus by usage of clot-dissolving agents. The necessities of such safer compounds are to be critically examined for thrombolytic activity especially, from marine sources. Thrombolytic agents have been investigated as a possible treatment for thrombus. The aim of this study was to investigate the in vitro thrombolytic potential of Streptomyces sp.VITJS4 (NCIM No. 5574); (ACC No: JQ234978.1) active compounds. The fibrin degradation revealed a clear transparent zone of clearance with 500μg/mL concentration showing 24mm hydrolysis. The thrombolytic effect of Streptomyces sp.VITJS4 compounds was also demonstrated in vitro clot lysis assay where The percent of thrombolysis by the crude extract showed 90±1.7% at the concentration of 1000µg/mL,  whereas percent of thrombolysis by streptokinase was found 100± 00%%. The bioactive compounds were further studied for spectrophotometric analysis. The UV-VIS profile showed different peaks ranging from 400-700 nm with different absorption respectively. The data confirmed the presence of both analogues with absorption maxima at 210 and 310 nm. A sensitive method using LC-MS technique was optimized for the separation and identification of bioactive metabolites which was indicated by the fingerprints. The results of the LC-MS analysis provided different peaks determining the presence of compounds with different therapeutic activities.  The current study refers the bioactive compound as impressive thrombolytic agent for further laboratory study. Further studies should be conducted to ensure the efficacy and safety of different concentration of bioactive compounds for drug development. Hence the results reported perhaps useful for the discovery of novel thrombolytic drugs from marine origin
Molecular identification of autochthonous bacterial fibrinolytic protein producers from fermentative food preparations
No full text154-162The current study investigates screening, isolation and characterization of fibrinolytic enzyme producing autochthonous
bacteria from fermented foods. About 21 different fermented food samples were screened for fibrinolytic protease producing
bacteria. Procured and prepared samples were homogenized, serially diluted and plated onto nutrient agar and soybean
casein digest agar medium. A total number of 89 isolates were isolated and screened for caseinolytic and fibrinolytic
activities. Out of those 89 isolates, nineteen were found to be potent protease producers. Amongst those isolates MS2, MS3,
MS4 and MS9 had notable fibrinolytic activities as well. These isolates were subjected for biochemical and molecular
characterization to determine their taxonomy and phylogeny. The strain with highest fibrinolytic potency was found to be
Bacillus subtilis VITMS2 isolated from fermented milk of Vigna unguiculata. The clot lysing ability of the selected potent
strain B. subtilis was found to be 79.98%. The total and specific activity of the same was found to be 788.82 U/mL and
41.73 U/mg, respectively. Other strains with comparatively low fibrinolytic activity were Pseudomonas aeruginosa
VITMS3, Bacillus sp VITMS4 and Alcaligenes sp. VITMS9
Novel Fibrinolytic Protease Producing Streptomyces radiopugnans VITSD8 from Marine Sponges
No full textFibrinolytic enzymes have received more attention due to their medicinal potential for thrombolytic diseases. The aim of this study is to characterize the in vitro fibrinolytic nature of purified protease producing Streptomyces radiopugnans VITSD8 from marine brown tube sponges Agelas conifera. Three varieties of sponge were collected from the Rameshwaram Sea coast, Tamil Nadu, India. The fibrinolytic activity of Streptomyces sp. was screened and determined by casein plasminogen plate and fibrin plate methods respectively. The crude caseinolytic protease was purified using ammonium sulfate fractionation, affinity and ion-exchange chromatography. Based on the morphological, biochemical, and molecular characterization, the isolate VITSD8 was confirmed as Streptomyces radiopugnans. Maltose and peptone were found to be the best carbon and nitrogen sources for the production of fibrinolytic protease. The carbon and nitrogen source peptone showed (781 U/mL) enzyme activity. The optimum pH and temperature for fibrinolytic protease production was found to be 7.0 and 33 °C respectively. The purified enzyme showed a maximum specific activity of 3891 U. The blood clot lysis activity was compared with the standard, and it was concluded that a minimum of 0.18 U (10 µL) of purified protease was required to dissolve the blood clot. This is the first report which exploits the fibrinolytic protease activity of Streptomyces radiopugnans VITSD8 extracted from a marine sponge. Hence the investigation suggests a potential benefit of purified fibrinolytic protease which will serve as an excellent clot buster alternative
