1,721,368 research outputs found
Orbital liposarcoma in a dog : a case report
No full textPurpose: To report a case of primary canine orbital liposarcoma. Methods: A middle
aged female-spayed mongrel dog, from the public kennel, was referred with a history of
swelling and opacity of the left eye that had worsened over the past 2 months. Results: No
abnormalities were detected on general clinical examination. Mouth opening was not painful
and examination of the oral cavity was unremarkable. Ophthalmic examination showed
severe left exophthalmos with reduced globe retropulsion and central exposure keratitis. Corneal
opacification prevented posterior segment examination. An orbital space occupying
lesion was diagnosed, further diagnostic tests were declined by the kennel handler due to
financial constraints. Enucleation was performed. A retrobulbar, multilobulated mass,
2.5 9 3 9 3 cm, was excised along with the globe and formalin- fixed. Histologically, the
mass was composed of dense sheets of large polygonal cells separated by thin fibrous stroma.
Cells have well defined borders, abundant vacuolated cytoplasm and small, peripheral nuclei.
Cell atypia was mild, mitoses <1/10 HPF. Immunohistochemically neoplastic cells were negative
for pancytokeratin and desmin, positive for vimentin and S100; a well differentiated
orbital liposarcoma was therefore diagnosed. The dog recovered uneventfully from surgery
and no recurrence has been noted after 8 months. Conclusions: Liposarcoma is a common
soft-tissue sarcoma but only rarely detected in the orbit. In men, its outcome is dependent
on tumor dimension (<5 cm) and histologic subtype. In the present case neither recurrence
nor signs of metastases were detected 8 months from diagnosis
Histological and immunohistochemical features of a recurrent epithelial-myoepithelial lacrimal gland tumor in a dog
No full textPurpose: To describe histological and immunohistochemical features of a rare lacrimal
gland tumor. Methods: A conjunctival mass in the upper eyelid, lateral cantus, was surgically
excised from an 11 year-old female mongrel dog. Eight months later the dog presented
with recurrence of a larger orbital mass, and complete orbital exenteration was performed.
Samples from both surgeries were formalin fixed and submitted for histology. Microtomic
sections were stained with hematoxylin and eosin and serial sections immunostained with the
standard ABC method in order to characterize neoplastic cells. Monoclonal antibodies antivimentin,
cytokeratin 14 (CK14), CK19, CK8/18 and alpha smooth-muscle-actin (aSMA)
were applied. AEC served as the chromogen. Results: Histologically, the first mass excised
was a large, capsulated, neoplastic nodule with expansive growth. The second mass was a
large multilobulated un-encaspulated nodule with multifocal signs of infiltrative growth. In
both tumors two distinct cell populations were present: cuboidal cell lining small ductular
structure and elongated cells with scant, clear cytoplasm disposed in short bundles or in
whorls around ductal structures. Cuboidal cells were positive for CK19 and CK8/18, spindle
cells were CK14 and aSMA positive. Both cell types were vimentin negative. Based on histological
and immunohistochemical feature a diagnosis of epithelial-myoepithelial carcinoma
(ECM) was posed. Conclusions: ECM is a rare tumor of lacrimal gland, whose histological
features parallel those of salivary tumors, in which both component of secretory parenchyma,
i.e. epithelial and myoepithelial cells, undergo neoplastic transformation. Further
investigations are necessary to understand the specific prognostication criteria for this type
of neoplasm
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Bilateral complex microphthalmia with intraocular dermoid cyst in a neonate red deer (Cervus Elaphus)
No full textA 3-day-old, male red deer (Cervus
elaphus) with bilateral microphthalmia was found dead in the Western Alps in northern Italy. No other gross alterations were present.
Ocular globes were formalin fixed and processed for histology. In both eyes, a large cyst, filled with keratin and lined by squamous epithelium admixed with sebaceous glands and hair follicles, expanded the anterior chamber.
A mass composed of spindle cells and small tubules, embedded in myxoid ground substance, filled the vitreal chamber. No lens tissue was found, leading to the speculation that
the dermoid cystic structures originated from abnormal differentiation of the primitive ectodermal lens vesicle. Immunohistochemistry with a panel of antibodies against cytoskeletal
proteins revealed neural and mesenchymal
elements within the vitreal mass. A complex bilateral ocular dysgenesis, with microphthalmia, aphakia, and dermoid metaplasia of primitive lens vesicle was diagnosed. The latter
finding was unusual and was previously documented only in Texel sheep (Ovis ammon), in which it was related to specific gene mutation on chromosome 23
Indicatori istologici di malignità nei tumori melanocitari uveali del cane
No full textI tumori melanocitari sono le neoplasie primarie oculari più frequenti nel cane; più spesso benigni, sono
tuttavia segnalati casi di metastasi da melanomi uveali. Il principale criterio istologico prognostico è
l’indice mitotico (IM), mentre l’invasione dei vasi sclerali è controversa, considerata ora estensione ai
canali preformati di deflusso dell’acqueo, ora espressione di aggressività della neoplasia.
Scopo del presente lavoro è definire parametri istologici di significato prognostico. 18 melanomi oculari
da 18 cani sono stati processati per l’istologia, colorati con ematossilina eosina ed immunocolorati con
anticorpi (mAb) anti MMP2; MMP9 e Ki67. Sono stati valutati l’indice mitotico (IM), l’indice di
proliferazione (IP = n° nuclei KI67+/n° nuclei totali), l’estensione della necrosi e l’infiltrazione
linfocitaria intratumorale. In base ai dati di follow up (14/18): 9 cani erano in salute, 5 deceduti: 3 per
cardiopatia e 2 con segni di metastasi encefaliche. 16 neoplasie erano masse nodulari, 2 rivestivano
l’intera uvea. Cellule neoplastiche invadevano i vasi sclerali in 11 casi, 13 avevano foci di necrosi, 6
linfociti intratumorali, l’IM solo in 3 casi era >1. La reattività immunoistochimica dei mAb anti-MMPs,
ma non quella di Ki67, risultava alterata dal trattamento di decolorazione. L’IP variava da 4 a 38,7%
(media 19,7).
In base ai dati raccolti tra le caratteristiche istologiche considerate solo l’indice mitotico era correlato alla
prognosi. La frequenza dell’invasione dei vasi sclerali, anche in tumori benigni, avvalorava l’ipotesi che
essa fosse in relazione alla struttura anatomica dell’angolo di filtrazione. Infine, i 2 soli tumori con
prognosi infausta presentavano un pattern di crescita intraoculare inusuale nel cane ma correlato,
nell’uomo, ad una prognosi negativa.CANINE UVEAL MELANOCYTIC TUMOURS: HISTOLOGICAL INDICATORS OF
MALIGNANCY
Uveal melanocytic tumor is the most common primary intraocular neoplasm in the dog. Although mostly
benign, metastasizing malignant tumors were reported. Currently, mitotic index (MI) is considered the
most reliable histologic criteria for prognosis, whereas it is controversial if tumor invasion of scleral
vessel is the extension through preformed aqueous flow canals, or a true vessel invasion. Aim of this
report was to define histological parameters of prognostic value. 18 eyes, from 18 dogs, were processed
for histology, stained with hematoxylin and eosin and immunostained with mAbs anti-MMP2, MMP9 and
Ki67 (MIB1). MI, proliferative index (MIB1+ nuclei/total nuclei *100), intratumoral necrosis and
lymphocytes infiltration were evaluated.
Based on follow up data (14/18): 9 dogs were healthy, 5 died: 3 for cardiopathy and 2 with a diagnosis of
metastatic brain diseases. 16 tumors were a single mass, 2 lined circumferentially the uvea. Scleral
vessels were invaded in 11 cases, necrosis was present in 13, lymphocytes in 6. In only 3 cases MI was
>1. Bleaching of sections affected immunostaining with MMPs (false positive signal), Ki67 was
unaltered. IP ranged 4-38.7% (media 19.7).
Among the histological features considered, only MI was prognostic. Neoplastic invasion of scleral vessel
was frequent, most likely related to the anatomical structure of the filtration angle rather than an indicator
of malignancy. In the present cases, both tumors with a negative prognosis showed a circumferential
pattern of growth, a pattern unusual for canine melanomas while it is related with a worst prognosis in
human beings
Five cases of histiocytic disorder in golden retriever dogs
No full textPurpose: To report the clinical and histopathological aspect of ocular histiocytic lesions in five Golden Retriever dogs. Methods: Three cases of conjunctival proliferative lesions and two cases of conjunctival proliferative lesions associ- ated with the presence of cutaneous nodules, in five Golden Retriever dogs aged between 11 months and 3 years are reported. A complete ophthalmological examination was performed on all subjects showing conjunctival chemo- sis associated with the presence of nodular lesions in the bulbar and palpebral conjunctiva of the third eyelid; in two subjects the presence of nodules in the skin was also ob- served. Surgical biopsy of lesions, both ocular and cutane- ous, was taken in all dogs and specimens were submitted for histology.
Results: In four subjects histological findings were consist- ent with systemic histiocytosis while in one dog histology was suggestive of histiocytic proliferative lesion. One case presented, histologically, with marked angiotropic and an- gioinvasive organization of infiltrating histiocytes. This pa- tient died 6 months after the first presentation.
Conclusions: Histiocytic proliferative disorder should be considered as differential diagnosis in Golden Retriever dogs that present with nodular conjunctival lesions
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