493 research outputs found
An Analysis and Evaluation of the Graduate Training Program For Engineers At the Allis-Chalmers Manufacturing Company
No full textThe purpose of this thesis was to evaluate the Graduate Training Program for engineers at the Allis-Chalmers Manufacturing Company, from its initial stages up to the present time.
It is hoped that the discussion of this problem will be beneficial to the Company as well as to colleges and universities in the future determination of their curricular patterns for industrial engineering
Allis, D., et. al. / Editor Epigenetics
No full textC. David Allis, editor. Epigenetics
Second edition
The regulation of gene expression in many biological processes involves epigenetic mechanisms. In this new volume, 24 chapters written by experts in the field discuss epigenetic effects from many perspectives. There are chapters on the basic molecular mechanisms underpinning epigenetic regulation, discussion of cellular processes that rely on this kind of regulation, and surveys of organisms in which it has been most studied. Thus, there are chapters on histone and DNA methylation, siRNAs and gene silencing; X-chromosome inactivation, dosage compensation and imprinting; and discussion of epigenetics in microbes, plants, insects, and mammals. The last part of the book looks at how epigenetic mechanisms act in cell division and differentiation, and how errors in these pathways contribute to cancer and other human diseases. Also discussed are consequences of epigenetics in attempts to clone animals. This book is a major resource for those working in the field, as well as being a suitable text for advanced undergraduate and graduate courses on gene regulation.https://digitalcommons.rockefeller.edu/ru-authors/1004/thumbnail.jp
Increased phosphorylation of histone H1 in mouse fibroblasts transformed with oncogenes or constitutively active mitogen-activated protein kinase kinase
No full textWe compared the nucleosomal organization, histone H1 subtypes, and histone H1 phosphorylated isoforms of ras-transformed and parental 10T1/2 mouse fibroblasts. In agreement with previous studies, we found that ras-transformed mouse fibroblasts have a less condensed chromatin structure than normal fibroblasts. ras-transformed and parental 10T1/2 cells had similar amounts of H1 subtypes, proteins that have a key role in the compaction of chromatin. However, labeling studies with P-32 and Western blot experiments with an antiphosphorylated H1 antibody show that interphase ras-transformed cells have higher levels of phosphorylated H1 isoforms than parental cells. G(1)/S phase-arrested ras-transformed cells had higher amounts of phosphorylated H1 than G(1)/S phase-arrested parental cells. Mouse fibroblasts transformed with fes, mos, raf, myc, or constitutively active mitogen-activated protein (MAP) kinase kinase had increased levels of phosphorylated H1. These observations suggest that increased phosphorylation of H1 is one of the consequences of the persistent activation of the mitogen-activated protein kinase signal transduction pathway. Indirect immunofluorescent studies show that phosphorylated H1b is localized in centers of RNA splicing in the nucleus, suggesting that this modified H1 subtype is complexed totranscriptionally active chromatin.PT: J; CR: ASHIHARA T, 1979, METHOD ENZYMOL, V58, P248 AUBERT D, 1991, J CELL BIOL, V113, P497 AVRUCH J, 1994, TRENDS BIOCHEM SCI, V19, P279 BASSIMHASSAN A, 1994, J CELL SCI, V107, P425 BELIKOV SV, 1993, NUCLEIC ACIDS RES, V21, P1031 BLENCOWE BJ, 1994, J CELL BIOL, V127, P593 BLOSMANIS R, 1987, CANCER RES, V47, P1273 BLUMER KJ, 1994, TRENDS BIOCHEM SCI, V19, P236 BRADBURY EM, 1992, BIOESSAYS, V14, P9 BRENEMAN JW, 1993, EXP CELL RES, V206, P16 CARTER KC, 1991, J CELL BIOL, V115, P1191 CARTER KC, 1993, SCIENCE, V259, P1330 COLE RD, 1987, INT J PEPT PROT RES, V30, P433 DAKSIS JI, 1994, ONCOGENE, V9, P3635 DAVIE JR, 1982, ANAL BIOCHEM, V120, P276 DAVIE JR, 1987, CANCER RES, V47, P5407 DAVIE JR, 1991, BIOCHEM J, V280, P491 DAVIS RJ, 1993, J BIOL CHEM, V268, P14553 DELCUVE GP, 1989, BIOCHEM J, V263, P179 DELCUVE GP, 1992, ANAL BIOCHEM, V200, P339 DEVOTO SH, 1992, CELL, V68, P167 DURFEE T, 1994, J CELL BIOL, V127, P609 EGAN SE, 1987, MOL CELL BIOL, V7, P830 EGAN SE, 1987, SCIENCE, V238, P202 ERICSSON C, 1990, CELL, V60, P73 FILMUS J, 1994, ONCOGENE, V9, P3627 GARRARD WT, 1991, BIOESSAYS, V13, P87 GORKA C, 1993, EXP CELL RES, V205, P152 HILL CS, 1990, EMBO J, V9, P805 HILL CS, 1991, EMBO J, V10, P1939 HIRANO T, 1994, CELL, V79, P449 HOHMANN P, 1983, MOL CELL BIOCHEM, V57, P81 HUANG S, 1991, GENE DEV, V5, P2288 HUANG S, 1992, P NATL ACAD SCI USA, V89, P305 HUANG S, 1994, J CELL BIOL, V126, P877 HUNTER T, 1994, CELL, V79, P573 JACKSON DA, 1993, EMBO J, V12, P1059 JANSENDURR P, 1993, P NATL ACAD SCI USA, V90, P3685 KAMAKAKA RT, 1990, EMBO J, V9, P3997 KAPLAN LJ, 1984, J BIOL CHEM, V259, P8777 LAITINEN J, 1990, J CELL BIOL, V111, P9 LAITINEN J, 1995, J CELL BIOCHEM, V57, P1 LANGECARTER CA, 1994, SCIENCE, V265, P1458 LENNOX RW, 1982, J BIOL CHEM, V257, P5183 LENNOX RW, 1983, J BIOL CHEM, V258, P262 LENNOX RW, 1988, BIOCH CELL BIOL, V66, P636 LENNOX RW, 1988, HISTONE GENES GENE E, P375 LIN RL, 1991, GENE DEV, V5, P1601 LU MJ, 1994, CHROMOSOMA, V103, P111 MANSOUR SJ, 1994, SCIENCE, V265, P966 MCCLARTY GA, 1990, J BIOL CHEM, V265, P7539 NACHEVA GA, 1989, CELL, V58, P27 NAGARAJA S, 1995, BBA-GENE STRUCT EXPR, V1260, P207 NEVINS JR, 1992, NATURE, V357, P375 NICKEL BE, 1987, BIOCHEMISTRY-US, V26, P4417 OHSUMI K, 1993, SCIENCE, V262, P2033 PARSEGHIAN MH, 1993, CHROMOSOME RES, V1, P127 PARSEGHIAN MH, 1994, CHROMOSOMA, V103, P198 PARSEGHIAN MH, 1994, PROTEIN SCI, V3, P575 POSTNIKOV YV, 1991, NUCLEIC ACIDS RES, V19, P717 RESNITZKY D, 1994, MOL CELL BIOL, V14, P1669 ROTH SY, 1992, TRENDS BIOCHEM SCI, V17, P93 TAN KB, 1982, J BIOL CHEM, V257, P5337 VANHOLDE KE, 1988, CHROMATIN VANHOLDE KE, 1992, J BIOL CHEM, V267, P2837 WAN KM, 1994, P NATL ACAD SCI USA, V91, P594 WEINTRAUB H, 1984, CELL, V38, P17 WEINTRAUB SJ, 1992, NATURE, V358, P259 XING YG, 1993, SCIENCE, V259, P1326 YASUDA H, 1981, BIOCHEMISTRY-US, V20, P4414; NR: 70; TC: 60; J9: J BIOL CHEM; PG: 8; GA: RQ991Source type: Electronic(1
Dephosphorylation of the C-terminal tyrosyl residue of the DNA damage-related histone H2A.X is mediated by the protein phosphatase eyes absent
No full textIn mammalian cells, the DNA damage-related histone H2A variant H2A.X is characterized by a C-terminal tyrosyl residue, Tyr-142, that is phosphorylated by an atypical kinase, WSTF. The phosphorylation status of Tyr-142 in H2A.X has been shown to be an important regulator of the DNA damage response by controlling the formation of �H2A.X foci, which are platforms for recruiting molecules involved in DNA damage repair and signaling. In this manuscript, we present evidence to support the identification of the Eyes Absent (EYA) phosphatases, protein tyrosine phosphatases of the haloacid dehalogenase superfamily, as being capable of dephosphorylating the C-terminal tyrosyl residue of histone H2A.X. We demonstrate that EYA2 and EYA3 displayed specificity for Tyr-142 of H2A.X in assays in vitro. Suppression of eya3 by RNA interference resulted in elevated basal phosphorylation, and inhibited DNA damage-induced dephosphorylation, of Tyr-142 of H2A.X in vivo. The study provides the first indication of a physiological substrate for the EYA phosphatases and suggests a novel role for these enzymes in the regulation of the DNA damage response
Dr. D. Hurlbut Allis.
No full textEditors: Aug. 1859-July 1865, J. D. White, J. H. McQuillen, G. J. Ziegler.--Aug. 1865-Dec. 1871, J. H. McQuillen, G. J. Ziegler.--Jan. 1872-May 1891, J. W. White.--July 1891-Apr. 1930, E. C. Kirk (with L. P. Anthony, Dec. 1917-Apr. 1930).--May 1930-Dec. 1936, L. P. Anthony.Vols. 1-13 are called "new series."Merged in Jan. 1937 with: Journal of the American Dental Association, ISSN 1048-6364, to form: Journal of the American Dental Association and dental cosmos, ISSN 0375-8451
Caracterização da arquitetura de planta em genótipos de algodoeiros portadores do carater "CLUSTER" e normal em diferentes espaçamentos.
No full textAquaculture, conservation and restoration of anadromous fish populations of River Rhine with particular regard to the re-introduction of the Allis shad Alosa alosa
No full textThe largest population of the anadromous Allis shad (A. alosa) of the 19th century was found in River Rhine and has to be considered extinct today. To facilitate the return of A. alosa into River Rhine an EU LIFE-project was initiated in 2007. The overall objective of this thesis was to assist aquaculture and stocking-measures at River Rhine, as well as to support restoration and conservation of populations of Allis shad in Europe.
By culturing the free-swimming nematode T. aceti in a solution of cider vinegar we developed a cost-effective live food organism for the larviculture of fish. As indicated by experiments with C. maraena, T. aceti cannot be regarded as an alternative to Artemia nauplii. However it has to be considered a suitable supplemental feed in the early rearing of C. maraena by providing essential fatty acids, thereby optimizing growth.
Also mass-marking practices with Oxytetracycline, as they are applied in the restocking of Allis shad have been evaluated. In experiments with D. rerio we demonstrated that water hardness can detrimentally affect mortality during marking and has to be considered crucial in the development of marking protocols for freshwater fish.
In order to get independent from wild spawners an ex-situ Broodstock-facility for Allis shad was established in 2011. Upon examination of two complete year classes of this broodstock, we found a high prevalence of various malformations, which could be traced back to distinct cysts developing one month post hatch. Despite applying a variety of clinical tests we could not identify any infectious agents causing these malformations. The observed malformations are probably a consequence of suboptimal feeding practices or the properties of the physio-chemical rearing environment.
The decline of stocks of A. alosa in Europe has been largely explained with the increase of river temperatures as a consequence of global warming. By investigating the temperature physiology of larval Allis shad we demonstrated that A. alosa ranges among the most thermo-tolerant species in Europe and that correlations between rising temperatures and the disappearance of this species have to be understood in a synecological context and by integrating a variety of stressors other than temperature. By capturing and examining juvenile and adult Allis shad from River Rhine, we demonstrated the first natural reproduction of A. alosa in River Rhine since nearly 100 years and the success of stocking measures within the framework of the LIFE project
Test 635: Allis-Chalmers D-17 Gasoline
No full textEXPLANATION OF TEST REPORT: ALLIS-CHALMERS D-17 GASOLINE TEST A: The manufacturer\u27s representative operates the tractor for a minimum of 12 hours using light to heavy drawbar loads in each gear. This serves as a period for limber up, general observation and adjustments. Adjustments that are permissible include valve tappet clearance, breaker ,point gap, spark plug gaps, clutch and others of a similar nature. No new parts or accessories can be installed without having mention made of it in the report. No data\u27 are recorded during this preliminary run except the time that the engine is operated. BELT HORSEPOWER TESTS TEST B: The throttle valve is hdd wide opened and the belt load on the dynamometer is adjusted so that the engine is at the rated speed recommended by the manufacturer. Carburetor, ignition timing and manifold adjustments are all set for maximum engine power. This test is designed to determine maximum belt horsepower of the tractor at ra.te.d speed and to measure fuel consumption at the maximum power on the belt. TEST C: For tractors with carburetors the best fuel economy does not always occur when the engine develops maximum power at rated speed. Test C is intended to allow the manufacturer\u27s representative to select a more economical fuel setting even though there is a slight loss of power. This more practical carburetor setting is used in all later tests except test F. The throttle valve is held wide open and load adjusted to give rated rpm. Tests Band C are the same for diesel tractors, which have an altogether different fuel system. TEST D: The throttle control lever is set so that the governor will maintain rated engine speed when rated load is applied. Rated load is 85% of 100% maximum, as obtained in test B, corrected to standard conditions. This rating is somewhat less than the maximum belt horsepower in order that the operator may have a certain amount of reserve. TEST E: Varying load serves to show the range of engine speeds when the engine is controlled by the governor during the following varied loads of 20 minutes each: rated load, no load, 1/2 rated load, maximum load at wide open throttle valve, 1/4 and 3/4 rated load. The average result of this test shows the average power and fuel consumption. Since the average tractor is subjected to varying loads, these data serve well in predicting fuel consumptionand efficiency of a tractor in general use. Torque, lb-ft at dynamometer, is obtained with wide open throttle and sufficient load is applied to give several readings. DRAWBAR HORSEPOWER TESTS In all drawbar tests the pull exerted by the tractor is transmitted by a hydraulic pressure cylinder to a recording instrument in the test car. All tests are made on the same dirt test course which is maintained by grading, sprinkling and rolling so that it remains very nearly the same throughout the season. The same tires, wheels and weights are used for all tests except J and K. TEST F: A drawbar test, the results of which are used to determine the rated drawbar horsepower in test H. The carburetor is set to develop maximum power as in test B. The rated gear recommended by manufacturer as plow gear is used in this test. The drawbar load is adjusted to give rated engine speed. TEST G: Maximum drawbar horsepower is determined in each gear when the carburetor is set for fuel economy as in test C. The throttle valve is held wide open and the load is applied so that the engine runs at rated engine speed. When operating in low gear it is not uncommon for the tractor to develop less drawbar horsepower than in rated gear because of excessive wheel slippage. When excessive wheel slippage occurs the load is reduced until slippage approaches 16%. When the load is reduced it is necessary to operate the tractor engine at part throttle and control engine speed by governor action. TEST H: Intended to test the ability of the tractor to run continuously for 10 hours at rated drawbar horsepower and to determine the fuel consumption during that time. Rated drawbar horsepower is 75% of 10°% maximum drawbar horsepower (Test F), corrected to standard conditions. When operating at rated load the throttle control lever is set to maintain rated engine speed. This rating is less than maximum drawbar horsepower in order that the operator may have a certain amount of reserve. TEST J: The tractor is operated in rated gear with all added weight removed. This test shows the effect of the removal of added weight on the performance of the tractor when compared with test G. Removal of wheel weights generally increases wheel slippage and decreases drawbar horsepower. TEST K: Similar to test J except that the smallest tires and lightest wheels offered by the manufacturer are used
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