34 research outputs found
Fluorescence emission spectra of the products of Flu-(dC)–(dG)-TAMRA extension
No full text<p><b>Copyright information:</b></p><p>Taken from " synthesis of uniform poly(dG)–poly(dC) by Klenow exo fragment of polymerase I"</p><p>Nucleic Acids Research 2005;33(2):525-535.</p><p>Published online 26 Jan 2005</p><p>PMCID:PMC548336.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Polymerase extension assay was performed as described in . The spectra were recorded before (curve 1), and 10 (curve 2) and 25 (curve 3) min after initiation of the synthesis. Excitation was at 490 nm. Schematic presentation of corresponding double-stranded products of the synthesis, are indicated to the right; F denotes for Flu, T for TAMRA. A significant amount of energy transfer in Flu-(dC)–(dG)-TAMRA is apparent as a decrease in the contribution of the Flu donor and an increase in the contribution of the TAMRA acceptor. The latter is seen as an increased relative emission around 580 nm in spectra of Flu-(dC)–(dG)-TAMRA. Extension of Flu-(dC)–(dG)-TAMRA results in an increase of molecular distance between the dyes and, as a result, in increase of Flu emission. When the length of extended polymer reaches ∼20 bp (see ), a reduced amount of energy transfer is apparent (spectrum 2). Flu emission reaches maximum, when the length of extended polymer is equal to ∼30 bp (∼10 nm); no contribution of TAMRA emission is then seen
Investigations at the Sloan Site (41 SS 51) a stratified alluvial terrace site in San Saba County, Texas
No full textA Master's Thesis on excavations and results from Texas Tech Archaeology Field Schools 1993-1996 at the Sloan Site, a stratified alluvial terrace site with a burned rock component in San Saba County, Texas. Site stratigraphy and recovered artifacts are discussed in detail, along with comparisons to similar sites in Central Texas. Deposits range in age from Early Archaic to Late Prehistoric
Fluorescence emission spectra of the product of Flu-(dC)–(dG)-TAMRA extension
No full text<p><b>Copyright information:</b></p><p>Taken from "Synthesis of novel poly(dG)–poly(dG)–poly(dC) triplex structure by Klenow exo fragment of DNA polymerase I"</p><p>Nucleic Acids Research 2005;33(20):6515-6521.</p><p>Published online 27 Nov 2005</p><p>PMCID:PMC1292991.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> Polymerase extension assay was performed in 100 mM Tris-Acetate, pH 8.0, 1.2 mM MgCl, 5 mM DTT, and 1.0 mM dGTP, 20 µg/ml Klenow exo and 0.2 µM Flu-(dG)–TAMRA-(dC) duplex at 37°C as described in ‘Materials and Methods’. The spectra were recorded before (), and 60 min after () initiation of the synthesis by addition of Klenow exo. Excitation was at 490 nm. Notice that (A) and (B) have different vertical scales
Interview with Ally Condie
No full textAlly Braithwaite Condie is the author of the # 1 New York Times bestselling Matched series and the Edgar Award Finalist Summerlost, among other novels, and of several picture books, including Here. A former English teacher, Ally resides outside of Salt Lake City with her family, including her four children. She has an MFA from the Vermont College of Fine Arts and is the founder and director of the nonprofit WriteOut Foundation
A case study and account of a series of educational therapy sessions with a learning disabled adolescent boy
No full textIncludes bibliographical references (pages 178-184)This case study both investigated and attempted to alleviate the learning disabilities of an adolescent boy by means of a detailed case history and twenty-six educational therapy sessions.\ud
Information for the case history was gathered from personal interviews, educational therapy sessions, medical evaluation, psychological consultation, teachers, cumulative record files and psychometric evaluation. The information collected for the case history was presented under seven major headings: 1) Identifying Data, 2) Personal and Social Data, 3) Medical Data, 4) Developmental Data, 5) Psychological Data, 6) Psychometric Data and 7) Educational Data.\ud
Accounts of the educational therapy sessions were given as they occurred. Each session was presented in terms of what the author tried to accomplish, what progress actually ensured and, finally, what impressions or insights the author gained from that session.\ud
What resulted was a study of the interrelationship between the boy???s emotional problems and his learning disabilities.\ud
Tentative conclusions were advanced as to how these two factors acted negatively upon each other. Literature by professionals in the field was cited to support the author's contentions.\ud
Finally, recommendations were made in the hope that this boy's learning disabilities and emotional problems could be further alleviated
A case study and account of a series of educational therapy sessions with a learning disabled adolescent boy
No full textThis case study both investigated and attempted to alleviate the learning disabilities of an adolescent boy by means of a detailed case history and twenty-six educational therapy sessions. Information for the case history was gathered from personal interviews, educational therapy sessions, medical evaluation, psychological consultation, teachers, cumulative record files and psychometric evaluation. The information collected for the case history was presented under seven major headings: 1) Identifying Data, 2) Personal and Social Data, 3) Medical Data, 4) Developmental Data, 5) Psychological Data, 6) Psychometric Data and 7) Educational Data. Accounts of the educational therapy sessions were given as they occurred. Each session was presented in terms of what the author tried to accomplish, what progress actually ensured and, finally, what impressions or insights the author gained from that session. What resulted was a study of the interrelationship between the boy’s emotional problems and his learning disabilities. Tentative conclusions were advanced as to how these two factors acted negatively upon each other. Literature by professionals in the field was cited to support the author's contentions. Finally, recommendations were made in the hope that this boy's learning disabilities and emotional problems could be further alleviated.California State University, Northridge. Department of Education.Includes bibliographical references (pages 178-184
The Civil Rights Movement Advocating for Equality
No full textCover -- Title Page -- Copyright -- Contents -- Foreword -- Setting the Scene: A Timeline -- Introduction: Looking for Guidance -- Chapter One: The Shoulders of Giants -- Chapter Two: Freedom Versus Equality -- Chapter Three: The Fight for Justice -- Chapter Four: A New Era in Civil Rights -- Chapter Five: Battling Segregation -- Chapter Six: Holding On to Power -- Epilogue: An Unending Battle -- Notes -- For More Information -- Index -- Picture Credits -- About the Author -- Back CoverDescription based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
The Canadian International Trade Tribunal : Canada's emerging trade jurisprudence
No full textEstablished in 1988, the Canadian International Trade Tribunal (the "CITT") replaced the Tariff Board, the Canadian Import Tribunal and the Textile and Clothing Board. Tasked with the responsibilities of advising the government on various trade related matters, conducting injury inquiries and reviewing certain decisions of the customs department, the CITT is an important source of Canadian trade policy and jurisprudence. This paper focuses on the role the CITT has played in the development of Canadian trade jurisprudence, with particular emphasis on the CITT's material injury inquiries and its appellate review of Canada Customs' classification and valuation determinations. Placing these decisions against the background of Canada's international trade commitments, the author gives a mixed review of the CITT's performance to date. That said, the author notes that a significant proportion of the CITT's failures in this area is more accurately attributable to the statutory limitations to its jurisdiction due to the incomplete manner in which Parliament has implemented Canada's international trade commitments into domestic law
BEAST Mode: Two seasons of archaeological survey on the gallon Jug-Laguna Seca Property in northwestern Belize
No full textTo address the lack of systematic survey in their permit area and record archaeological evidence of prehistoric and historic occupation, the Chan Chich Archaeological Project (CCAP) formed the Belize Estates Archaeological Survey Team (BEAST) before the 2013 field season. During the 2013 and 2014 field seasons, BEAST conducted pedestrian and UAV survey across the project’s permit area, which spans the Gallon Jug and Laguna Seca Ranches in northwestern Belize. Although archaeologists have studied the southern Maya lowlands for more than 100 years, the project area has been subject to limited archaeological survey, and this project represents the largest-scale systematic survey project ever undertaken on the property.
BEAST’s surveys completed over 80 km of linear survey, covering an area of over 2.1 km². Survey crews, led by the author, recorded 275 structures along American Seismic Lines, 356 total features, and 4 new sites (Ix Naab Witz, La Luchita, Montaña Chamaco, and Sylvester Village), and revisited six previously-recorded sites.
This thesis compares data recovered from these surveys with data from nearby sites such as Dos Hombres, La Milpa, and Guijarral. Various aspects of the recorded Maya settlement are examined, including ecological setting, structure size, and group type. An updated site inventory for the Gallon Jug-Laguna Seca property is included, as are maps of all revisited sites and surveyed areas
